[Histonet] Leica CV 5030 coverslipper and Fisherbrand Permount

it for manual cover slipping) for a new bottle, but has anyone tried to re-dilute it with toluene and put it back into the machine? If so, how do you do it? Thanks so much, Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Dept of Pharmacology Toxicology Rutgers, the State University of NJ 41

Re: [Histonet] Can't log into Histonet to do anything

I tried just now via Safari, and got this error message: Gateway Timeout: can't connect to remote host. Hopefully the IT people can fix it. Kathleen I got Gayle's email as well, but when I went to Histonet's page though Firefox and Google, got the Unable to Connect message when I clicked on

[Histonet] Thermo Scientific Richard-Allan Mark-it dyes

Thanks and have a good weekend, Kathleen Roberts Principal Lab Technician Histopathology Lab Office of Translational Sciences Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445-6905 ___ Histonet

[Histonet] Slide and cassette printers?

What are all of you using these days? What would be good for a small histo lab that has the potential to grow? (I am already aware of what Leica and Thermo have from the archives here, but I am still interested in your opinions, of course.) Has anyone tried the printers, labels and attachment

[Histonet] Sakura Tissue Tek TEC manual?

. We have pretty much figured it out, but it would be good to have one anyway.) Thank you so much! Kathleen Roberts Principal Lab Technician Histopathology Lab Office of Translational Sciences Rutgers, the State University of NJ 41 B Gordon Road Piscataway, NJ 08854 (848) 445-1443 FAX (732) 445

[Histonet] Re: User manual for Sakura Tissue Tek TEC 4710

Oho! Thank you very much, Jim! To the rest of Histonet, I withdraw my request for a manual. Now that I have that missing piece of information, I know just where to get it. Thanks anyway and have a great weekend! Kathleen Roberts Principal Lab Technician Histopathology Lab Office

Re: [Histonet] Paraffin Sections

Peri, Sounds like poor infiltration to me. What kind of tissue are we talking about, and what processing program did you use? Kathleen Principal Lab Technician Neurotoxicology Labs Dept of Pharmacology Toxicology Rutgers, the State University of NJ 41 B Gordon Rd Piscataway, NJ 08854

Re: [Histonet] Tissue Washing

Emily, The last time I had this problem, we switched to Ultrastick slides (ThermoFisher Scientific) and distilled water in the water bath. I haven't had any problems since with HE staining. Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Dept of Pharmacology Toxicology

Re: [Histonet] Minnows?

were you, I would first check with the person/organization that sent them and ask them what they want. Likely you will have to decal them, process them whole, and cut them to the level that they want. Good luck! Kathleen Roberts Principal Lab Technician Neurotoxicology Laboratories Molecular

Re: [Histonet] looking for contact info on Dolby-Jamison

From www.dolbeyjamison.com: Dolbey-Jamison provides product sales and service products_serviced.htm for all of Pennsylvania, New Jersey, Maryland, Delaware and the New York City Metropolitan area. index.html products_serviced.htm service__support.htm history.htm clients.htm

Re: [Histonet] OCT and RNA

dept. which has articles from the basics of RNA on up. Hope this helps! Kathleen Roberts Principal Lab Technician Neurotoxicology Labs Dept of Pharmacology Toxicology Ernest Mario School of Pharmacy Rutgers, the State University of NJ 41 B Gordon Rd Piscataway, NJ 08854 (732) 445-6914 Karen

Re: [Histonet] Destaining HE slides

Thanks to everybody for their destaining tips! -Kathleen Monfils, Paul wrote: Anything mildly basic will quickly destain eosin. Running tap water will do it, usually in 10 minutes or so. 0.1% NaOH or KOH in water or in 70% ethanol will do it quickly.

[Histonet] Destaining HE slides

I searched the archives and found Gary Gill's method for destaining HE slides, and for destaining eosin he calls for 1.5% NH4OH in 70% ethanolthe problem is, I don't have any NH4OH in the lab. Is there another way to fully destain eosin? Thanks, Kathleen Principal Lab Technician

Re: [Histonet] CNS Fixation

that I have tissue come off the Ultrastick slides during staining...but then I stain my slides manually since we don't have an autostainer yet. Good luck, Kathleen Roberts Principal Lab Technician Neurotoxicology Laboratories Dept of Pharmacology Toxicology Ernest Mario School of Pharmacy

[Histonet] Sections coming off of slides

which retrieval method he used, but I can find out if you need it). I've never tried to do this, and a search of the archives turned up nothing, but my keywords may be off.Has anybody managed to save a section that was coming off the slide? Thanks, Kathleen Roberts Principal Lab Technician

Re: [Histonet] Processing mouse seminal vesicles-thanks!

paraffin, use a mixture 1:1 of iso-propanol and paraffin, followed by the 2 paraffins. Try it and you will notice the difference (and the saings). René J. --- On Wed, 12/10/08, Kathleen Roberts [EMAIL PROTECTED] wrote: From: Kathleen Roberts [EMAIL PROTECTED] Subject: [Histonet] Processing

Re: [Histonet] Neurofilament Question

get a sample before you buy? I don't know if Covance does that, but it can't hurt to ask. https://store.crpinc.com/datasheet.aspx?UpProd=catalogno=SMI-312R Good luck, Kathleen Roberts Dept of Pharmacology Toxicology Rutgers University Peggy Bisher wrote: I have a student who asked if I could