[Histonet] Veterinary ISH

Hi Histonetters, I work in an animal research facility and we are trying to establish a working protocol for CISH on bovine tissues. So far we have tried a Dako PNA ISH kit, and had no luck on bovine tonsil tissue (have only tried once so far). We have also been trying to optimize the kit in

[Histonet] Thanks for the thoughts on Sequenza units!

Hello All, I have compiled all of the responses to date regarding the question I posted on histonet about too much volume and reverse capillary action: (I've changed font styles to differentiate between responses) I have used them for quite some time. I was having problems with uneven

[Histonet] Sequenza units for manual immunohistochemistry

their coverplates and the units themselves? Thanks in advance for your thoughts! Keri Keri Colwell, BSc Laboratory Technologist | Technologiste de laboratoire TSE and Pathology Lethbridge Laboratory | Laboratoire de Lethbridge Canadian Food Inspection Agency | Agence candienne d'inspection des

[Histonet] Waste disposal

Hi, I am currently working on ways to enhance my DAB stiaing and find I am generating two types of waste that I don't know how to deal with. One of my techniques involves post-DAB enhancement in a cupric sulfate solution. My other technique involves making my DAB solution and including some

[Histonet] DAB

for the tetrahydrochloride form, but again I used the free base and did get some reaction. Does anyone have thoughts on about how to get the free base to go into solution without oxidizing, or thoughts about free base versus the other form of DAB? Thanks! Keri Keri Colwell Laboratory Technologist