I can think of two things.
First, relentlessly make fun of your building planner for putting a histology
lab underneath a laundry. This is a mistake worthy of pointing and laughing.
Second, there are isolation tables and platforms. That's probably the first
thing I would try.
, residents, or
fellows can throw at them. I feel better having a sturdy piece of glass over
those sections.
Gerry
From: Caroline Miller [mailto:mi...@3scan.com]
Sent: Wednesday, November 04, 2015 7:05 PM
To: Keyser Gerald T
Subject: Re: [Histonet] Aqueous Mounting Media
Is there a reason you have
Currently my lab is using Aqua-mount (Lerner Laboratories). I'm looking at
replacing it with a different Aqueous mounting media. The only stain we use it
for is Oil Red O.
I've tried many different variations on its use and the results have been the
same.
1) The slide looks lovely the
Our lab uses 95% Ethanol. It takes longer to fix the tissue than formalin. But,
it's effective and far less toxic than formalin.
Gerry
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Manahil
Sent:
of resolution of
approx. 200nm. Cell membranes... what do they run... approx. 4nm?
Gerry
-Original Message-
From: Jamal [mailto:j.rowa...@alborglaboratories.com]
Sent: Tuesday, April 28, 2015 12:07 PM
To: 'Manahil'; Keyser Gerald T
Cc: histonet@lists.utsouthwestern.edu
Subject: RE
I can't find it without a paywall either. I find the idea of honey as a
formalin substitute fascinating.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Fawn Bomar
Sent: Wednesday, February 18, 2015 1:49
I would like to better seal my Oil Red O slides. The current process uses
aqueous mountant on the inside of the large coverslips and Permount on the
outside; which is incredibly messy and isn't actually that great of a seal.
The two options I'm looking at is:
1) Nail polish.
2)
GRAC
http://www.newcomersupply.com/product/gravity-recycling-alcohol-cartridge-tissue-processor-grac-tp
I've used this system. It takes quite a while to run. It works. But, there is
one big problem. If there is a possibility of Xylene or Propar contamination,
it will quickly ruin the filter
Voice to text cannot cope with doctors mumbling into recorders. Even the best
VRS only hits about 95% of the regular dictionary; which is very good. But, a
5% error rate is wildly unacceptable in a clinical environment.
Another parallel is trying to apply handwriting recognition software to a
Embedding:
I use a large, solid aluminum block that I keep cool in my cryostat. It's
approx. 9 in (length) x 4 in (width) x 3 in (depth). I just stick the epidermal
edge to the block and flatten it out so all margins are in contact with the
freezing surface. Drop some freezing medium on it and
I've only cut resin with a glass or diamond knife in an ultramicrotome. If you
are attempting to do it in a regular microtome, you would need a special blade
holder. I don't know if any microtome manufactures make glass knife holders.
You make the glass blades yourself using special glass.
We use a Leica CM1850 currently. May differer a bit. On a daily basis, we clean
the loose trimmings and wipe the inside with 95% ethanol. The exterior surfaces
are cleaned daily with caviwipes.
Once a month we turn off the machine, remove the microtome (I don't know if
this is proper for the
I'm toying with making my own aqueous mounting media. Although I will not
likely use this in actual cases, it would still be nice. Aqueous mounting media
is kind of expensive. But, there are some questions that need to be answered.
The Slides need to be kept for 10 years.
- Will the adhesive
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