Re: [Histonet] Anyone using Section Transfer System by Microm?

Thanks for bringing this to my attention, Ive never heard of it until now. I want one too, it does seem like an asset for microarrays, how much$ do they run? Morken, Timothy 11/14/13 11:58 AM Is anyone using the Thermo/Microm Secton Transfer System on their microtome?

RE: [Histonet] making 10 % EDTA

Thanks for your responses. Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 Pager#5029 (410) 328-5558 (410) 328-5508 fax https://cf.umaryland.edu/freezer/promo_pbr.cfm This e-mail and

[Histonet] making 10 % EDTA

Hi Histonetters, I have purchased a liquid EDTA solution that is .25M. I need to make 10% EDTA. Do I just dilute it 1/10 with DI water? Thanks Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD

[Histonet] Off Topic: researchers very funny

If this is a repost I apologize :) http://www.youtube.com/watch?v=Fl4L4M8m4d0feature=player_embedded This e-mail and any accompanying attachments may be privileged, confidential, contain protected health information about an identified patient or be otherwise protected from disclosure.

[Histonet] IHC OOps wrong secondary

Is it possible for me to remove the coverslip, run back to water and re-use the slides starting with the correct secondary? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410)

Re: [Histonet] IHC OOps wrong secondary

Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please consider the environment before printing this e-mail. Kimberly Tuttle ktut...@umm.edu 10/13/2010 12:07 pm Is it possible for me to remove the coverslip, run back to water and re-use the slides starting with the correct secondary

[Histonet] Arcturus Laser Capture Microdissection (LCM) Systems

Does anyone know who to contact to get this instrument serviced? It seems the company is no longer in business. The phone numbers I have dont work, and the website is no longer there. I found a company online who repairs them, but they are in Massachusetts, I am in Maryland so that would be my

[Histonet] HELP Starting a lab

I have a friend who is a Pathologist and starting a new lab. She sent me a list of start up supplies and equipment that she needs to purchase. Can I get some input on whats missing from her list, I've added a few things but Im going blank. Its a hisology lab for a urology practice if that

Re: [Histonet] IHC on frozens

this e-mail. Kimberly Tuttle ktut...@umm.edu 7/2/2009 12:55:47 pm Can you do heat retrieval on frozens? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax Please

Re: [Histonet] Budget Microarray - Finally

There is also a histonetter that makes silicone array molds, you can buy them online. http://www.arraymold.com/ Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax

[Histonet] Stat-Q Staining Kit

Does anyone use the Stat-Q Staining Kit by Innovex for multispecies IHC? Does it work as advertised? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This

[Histonet] microwave antigen retrieval

Can someone post instructions for microwave antigen retrieval... my pressure cooker broke and I need an immediate solution. Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410)

[Histonet] how long can slides stay in water or buffer

How long can you leave deparaffinized slides in water or buffer if you want to do IHC staining? Is leaving them in water or buffer preferable o letting them air dry? I was taught to never let sections dry out. Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University

RE: [Histonet] uncertified techs in Histology

LOL! I know what she means, I still have nightmares after 15 years! But Im glad I am certified because I was properly and thoroughly trained by TSgt. Nocito. Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore,

[Histonet] Pay and The histotech's piece of paper

I have been in histology for 14 years, and I have always been pleased with the pay. I never fished my degree, but I have always made comparable to or more than friends with degrees. Do most of you feel underpaid? I dont know how much MT's make compared to HT's. Kimberly C. Tuttle HT (ASCP)

RE: [Histonet] pathology assistants

LOL! You didnt answer the question and theres a Big Difference marilyn.a.we...@kp.org 01/28/09 8:09 AM 2 assistants. NOTICE TO RECIPIENT: If you are not the intended recipient of this e-mail, you are prohibited from sharing, copying, or otherwise using or disclosing its contents. If you

[Histonet] IHC Doublestain

I am supposed to try a doublestain using the DAKO autostainer where one of the antibodies requires a Proteinase K pretreatment and the other antibody requires antigen retrieval with heat. Should I do both, or will this not work at all? Thank you Kimberly C. Tuttle HT (ASCP) Pathology

[Histonet] Which Blocking reagent?

Im trying to work up a few IHC stains, but Im still learning too. Synaptopodin using a kidney control and Cleaved caspase 3 on tonsil. Im getting a lot of background but there are so many reagents out there I dont know which to try or whats the difference between them. Im using the Dako

Re: [Histonet] Offended

I dont think that anyone should assume that a tech is looking for another job because someone called the lab and asked for them. I get telemarketing calls from sales reps and emails and calls from job recruiters because most of them are members of this listserve, and many of our signature

[Histonet] Cleaved Caspase-3

Can anyone recommend control tissue? Thank You Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be

Re: [Histonet] ASCP HT maintenance fees

Really? I never pay to maintain HT certification. As far as I know theres a ASCP membership fee, but you dont have to be a member to be certified. Am I wrong here? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St

[Histonet] pronase vs. proteinase-k

Are they the same ? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410) 328-5508 fax This e-mail and any accompanying attachments may be privileged, confidential, contain

[Histonet] PLS Help with IHC protocol

Step 10: Add antibody at 10ug/ml in PBS + 0.1%BSA + 1:10 volume CAS block Can someone break this down for me in simple instructions? Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410)

[Histonet] ever heard of these antibodies?

For IHC: 1.Nephrin 2.m-TOR 3.caspases Have you ever used them on FFPE? did it work ? where did you but them? Thank you Kimberly C. Tuttle HT (ASCP) Pathology Biorepository and Research Core University of Maryland Room NBW58, UMMC 22 S. Greene St Baltimore, MD 21201 (410) 328-5524 (410)

RE: [Histonet] Dako Party

Andria, I have some photos, and since I took the photos, Im not in any of them. I had 1 too many Chardonnay but thankfully that was my last night, so I didn't have to face yall the next day. Can you send photos on histonet or must I send them directly to your email? Kimberly C. Tuttle HT