[Histonet] Repairing a damaged TMA block

Good afternoon Histonet! Does anyone have any experience repairing a precious TMA block that was damaged by heat? Part of the block is okay but half was exposed to heat and experienced some melting which misaligned some cores and has the block on a slant. We know that this has probably happened

[Histonet] Protocol for cutting frozen sections for RNA Sequencing

Hello Histonet! Can anyone share their protocol for cutting frozen section (rolls) that will be used for RNA sequencing downstream much like this nature article: https://www.nature.com/articles/s41591-020-0844-1 The only direction we have been given is to cut the sections at 100 microns. Knowi

Re: [Histonet] Looking for vendor for Zeus Wash solution

You might try searching under Michel Media or Michel's Media. It's the same > but some people refer to it as Zeus and some Michel. > Best, Kristyn > > > -- Forwarded message -- > From: Martha Ward-Pathology > To: "histonet@lists.utsouthwestern.edu" > > Cc: "histo...@lists2.utso

[Histonet] Histotechnologist Needed near Philly!

Hello Histo-netters! Busy hospital lab looking for a great histotech! Apply using the link below :) https://careers-towerhealth.icims.com/jobs/23469/histotechnologist/job Have a great day! ___ Histonet mailing list Histonet@lists.utsouthwestern.edu htt

[Histonet] Experience (especially long term)

Hello Histonetters! I'm looking to gather histotech's experience with Thermo Scientific's HistoStar embedding workstation. I am very familiar with Sakura and think it's top notch as far as service and support and, especially, longevity. How does everyone feel the HistoStar compares with the Tissue

Re: [Histonet] Histonet Digest, Vol 163, Issue 19

I would say it is highly dependent on the antibody and application you are using. We don't fix any of our frozen tissue and no antigen retrieval is necessary. However, whenever we receive specimens that have been through a full FFPE process then, yes, the same antibody requires some antigen retriev

Re: [Histonet] Giemsa Tissue control options

Charles, If you are looking for microorganisms you can try colon. Kristyn ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] C3 IF staining of FFPE tissue

Hello List, I suspect it may be a lost cause (since processing likely kills the antigen site) but has anyone had any luck with IF staining for C3 on FFPE tissue? I know frozen is ideal but occasionally, we need to revert to the FFPE sample. Best, Kristyn _