Re: [Histonet] Antibody diluent

What is everyone's take on what needs to be done from a verification/validation standpoint if you switch diluents for your concentrated antibodies? The CAP summary of recommendations on the validation IHC antibodies doesn't specifically mention the changing of diluent as one of the variables.

Re: [Histonet] Question about accessioning outside consult cases

Hi Martha, We have been using Beaker for over 2 years now. We created another accession that identifies cases that are being sent to us as a consult/referral (both Cyto and Histo). It works pretty well for us. Scott Scott A. Lindrud, MLS(ASCP)CT | Histopathology Technical

[Histonet] Weekend Coverage for Small Pathology Labs

Hi Histonet, I apologize if this is being re-posted but I did not see this message on the daily Histonet Digest so I'm not sure if it ever got posted. I was wondering if anyone would be willing to share their experience with weekend Histology coverage for smaller Pathology labs? When I

[Histonet] Fat Aspirates for Amyloidosis

Hi Histonetters, Would anyone be willing to share how they process a Fat Pad aspirate for Amyloidosis? Our method does not provide a very good result and we're looking to improve on how we do this. We collect direct aspirates using an 18 gauge needle and make air dried smears and stain with

[Histonet] MMR Negative controls

Good Morning HistoNet, Would anyone be willing to share what they use for control tissue for MMR testing? Finding negative expressing tumor is becoming more difficult for us to find and we're wondering if there is an alternate negative tissue that would be acceptable. NordiQC recommends

[Histonet] Negative IHC Control for Her2

Hi All, We are having an internal debate regarding Her2 IHC control tissue in our lab. We run a MTA (multi tissue array) consisting of 0+, 1+, 2+, and 3+ Her2 staining tissue taken from lumpectomy/resection cases. I'm in the process of searching for more tissue to use in future control

Re: [Histonet] Dako Artisan VS. Ventana Benchmark speical stainer

Miranda, We use the Dako Artisan for special staining and it performs great. We've been utilizing ours for about 7 years now. We use the following staining protocols: Iron PAS PAS/D GMS Retic Trichrome AFB Warthin Starry The staining is consistent and reproducible for all of our protocols.

[Histonet] Decal times for Bone Marrow Biopsies

Happy Friday to Everyone, I have a question about decal for Trephine collected bone marrows. We currently decal our Trephine collected bone marrow biopsies for 1 hour in Cal-Rite by Richard Allan, a mixture of formic acid and formaldehyde. Although this seems to work adequately most of the