Re: [Histonet] Use of sodium borohydride in immunofluorescence

n Wed, Mar 22, 2017 at 7:12 AM, Bob Richmond via Histonet < histonet@lists.utsouthwestern.edu> wrote: > > > > Lucie Guernsey at UC San Diego asks about sodium borohydride: > > >>I'm currently going over IHC SOPs that were written by lab members that > have lo

[Histonet] Use of sodium borohydride in immunofluorescence

have pregnancy brain and an obvious answer is slipping past me, but my current opinion is that any and all immunofluorescence done on paraformaldehyde (our usual fixative) fixed tissue should undergo sodium borohydride blocking. Am I missing something? Thanks for any help in clarifying this! Luc

[Histonet] Will dry ice damage paraffin blocks?

ackages or can I place a layer of paper towels or something in between the dry ice and paraffin blocks? Thanks, Lucie Lucie Guernsey UC San Diego Dept. of Pathology lguern...@ucsd.edu ___ Histonet mailing list Histonet@lists.utsouthwester

Re: [Histonet] Embedding Question

times and we haven't been able to put our finger on why. Anyone know if allowing infiltrated tissue to cool and then reheat before embedding is better or worse than keeping the tissue soaking in wax for hours at a time? Thanks! Lucie Lucie Guernsey UC San Diego lguern...@ucsd.edu On

Re: [Histonet] paraffin sectioning-dry tissue?

your tissue is an unknown variable. Am I overthinking this? Lucie Guernsey UC San Diego (858) 822-5797 lguern...@ucsd.edu On Thu, Feb 5, 2015 at 7:35 AM, James Watson wrote: > We use a 5% glycerin in denatured alcohol for our 100% alcohol on our > tissue processor for routine animal

Re: [Histonet] Mixing Paraffin Brands

infiltrate using Fisher's Paraplast Plus and embed using McCormick's Paraplast X-tra. Thanks again! Lucie Lucie Guernsey UCSD Dept. of Pathology lguern...@ucsd.edu On Wed, Jun 19, 2013 at 1:08 PM, Elizabeth Chlipala wrote: > That's what we do and we do not have any issues with

[Histonet] Mixing Paraffin Brands

the McCormick in the processor and the Fisherbrand in the embedder. Could that cause issues in the blocks as the tissue would be infiltrated with one brand and embedded in another? Maybe I'm over-thinking this...... Many thanks! Lucie Lucie Guernsey UCSD Dept. of Pat

Re: [Histonet] Which fixative for eyes

rk for us. Thanks, Lucie Lucie Guernsey UCSD lguern...@ucsd.edu On Wed, Jan 16, 2013 at 10:32 AM, Louro, Pedro < lou...@princeton.huntingdon.com> wrote: > Hello to the eye experts, > > > > I am currently working on a large animal (LA) eye project which will > give m

Re: [Histonet] Nerve teasing videos?

If the videos exist, I'm very interested in this as well. Thanks! Lucie Lucie Guernsey UCSD Dept. of Pathology lguern...@ucsd.edu On Fri, Nov 16, 2012 at 10:11 AM, Morken, Timothy < timothy.mor...@ucsfmedctr.org> wrote: > Hi all, does anyone know of instructional videos for

[Histonet] Anyone have a Revco UxF -80 freezer? Need some input.

this whole process again in a couple of months. Is our lab just insanely unlucky and happened to get 2 rare duds in a row or do these freezers have a reputation? Thanks so much! Lucie Lucie Guernsey UCSD Dept. of Pathology lguern...@ucsd.edu ___

Re: [Histonet] Fwd: Plants

I keep a pothos and spider plant in my lab. EH&S has never complained, though I can't say one way or another if it's technically allowed. While my plants are mostly just decorative (I don't think I have enough of them to make much of a difference), it doesn't hurt that they may be filtering our ai

[Histonet] Shiny side of a paraffin section

shiny side down? What would the difference be between a 'properly' collected section and a rebelliously collected shiny-side up section? Does it even matter? Thanks! Lucie Lucie Guernsey UC San Diego lguern...@ucsd.edu ___ Histo

[Histonet] Revco -80 Freezer Sliding Drawer Rack Reviews

e the sliding drawer racks and have an opinion about them? Should we just stick with the tried and true side-access racks? Thanks for your input! Lucie Lucie Guernsey UC San Diego lguern...@ucsd.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.

Re: [Histonet] CD31 for rat tissue

Colleen, Unfortunately, I can't help you out, but I'm also interested in this question. If you happen to get responses off-list, can you share which rat antibodies are suggested by others? Also, would you mind sharing which mouse antibody and dilution you found works well? Thanks! Lu

[Histonet] Ultra-Low Temp. Upright Freezer

Hi all! I'm hoping to get some help RE: purchasing a new ultra-low temp. upright freezer. I've tried searching Histonet, but many of the posts are a few years old, and my guess is that the products have changed a bit over the years. My lab is looking to purchase a large (absolute minimum size of

[Histonet] Re: Masson Trichrome

run more stains using your suggestions and, cross your fingers for me, hopefully (finally) optimize this stain for my lab Thanks again! Lucie Guernsey University of California, San Diego On Thu, Apr 16, 2009 at 3:07 PM, Lucie Guernsey wrote: > Hi Histonetters - I could really use you

[Histonet] Masson Trichrome

ne-time use, correct? Thank you in advance for all your suggestions! Lucie Guernsey University of California, San Diego ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet