[Histonet] Bone PMMA sections

2019-02-21 Thread MANTERO Stefano RIC via Histonet
Good morning Histonetters! I'm starting to cut out of bone samples included in PMMA. I would like some suggestion how to storage the slides cut. Many thanks in advance Stefano Dott. Stefano Mantero Human Genome Laboratory CNR-IRGB c/o Humanitas Research Hospital Via Rita Levi Montalcini (Ex

[Histonet] R: EDTA decalcification tissue issues

2017-11-14 Thread MANTERO Stefano RIC via Histonet
Good Morning Kelly, I work in a research institute that works with murine samples; In our laboratory we have adopted two descaling protocols: one with ion exchange resins/acid solution while the other uses EDTA. The EDTA descaling system is slower but keeps the nucleic acids much better. We use