[Histonet] monomeric myosin antibody for paraffin

Does anyone know of a source for an antibody to monomeric myosin that will work on human tissues? We have looked but haven't been able to find one. Any suggestions would be greatly appreciated. Thanks Mark ___ Histonet mailing list

[Histonet] Fixation of gelatin scaffold with glutaraldehyde

Has anyone had any experience fixing gelatin scaffolds in glutaraldehyde vapour. One of the people I am working with is trying to do this and was wondering if anyone out there has had any experience doing this. Any suggestions would be greatly appreciated. Thanks Mark

[Histonet] whole mount immunofluorescence staining for mouse aorta

A colleague of mine has asked if I have any experience wholemount immunofluorescence staining for mouse aorta. I don't have any experience with this so thought I would ask the group for any suggestions/protocols/ tips etc. Thanks Mark ___ Histonet

[Histonet] Re: Histonet Digest, Vol 124, Issue 20

We have one tech who does all of the steps in EM processing, staining etc., but we are a research lab. Mark Elliott Centre for Heart Lung Innovation Vancouver, BC Date: Wed, 19 Mar 2014 14:25:15 + From: Gauch, Vicki gau...@mail.amc.edu Subject: [Histonet] Question for Labs performing

[Histonet] Icam-1 antibody for mouse tissue.

Can anyone recommend an antibody to ICam-1 that is not made in a mouse. I need to stain mouse tissues for ICam-1 and would like very much to avoid having to do mouse-on-mouse. Thanks for all suggestions Mark ___ Histonet mailing list

[Histonet] Ceramide antibodies

I have been asked to do some staining for ceramide in human lungs. Anyone have recommendations for an antibody that works well on human tissues? Any tips would be greatly appreciated. This is for either frozen tissue or FFPE. Thanks Mark ___ Histonet

[Histonet] Re: Histonet Digest, Vol 118, Issue 42

Well done Peggy!!! Mark ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Antibodies to Cathepsin proforms

I hope everyone is gearing up for the NSH Meeting this weekend. Would love to be there but unfortunately can't make it this year. Hope you all have a great time. I have been asked if there are antibodies to the various Cathepsins which will differentiate between the proform and the active

[Histonet] Dispenser volumes

I too use the DAKO Autostainer and have for many years. My default setting is 100 ul and drop zone in the middle of the slide, however, I adjust the volume and the drop zone depending on how large the section is and where the section is on the slide in order to conserve reagents.I don't

[Histonet] CD163 for frozen human sections

Can anyone recommend a CD163 antibody that works on frozen human tissue? The two different ones that I have say not for frozen sections. any information greatly appreciated. Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the

[Histonet] CD31 for FFPE IHC on Mouse

Amy Can you please share the responses you got with the rest of us Thanks Mark Message: 8 Date: Thu, 18 Oct 2012 16:21:55 -0400 From: Amy Porter port...@msu.edu Subject: RE: [Histonet] CD31 for FFPE Immunohistochemistry on Mouse Model To: 'Amy Porter' port...@msu.edu,'Histonet'

[Histonet] Versican antibody for dog tissue

Can anyone recommend a versican antibody that works on dog tissue? Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination,

[Histonet] Rabbit M1 and M2 Markers

What are people using to differentiate between M1 and M2 cells in rabbit tissues?? Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination,

[Histonet] Re: Histonet Digest, Vol 89, Issue 12

Date: Tue, 12 Apr 2011 11:37:17 -0400 From: Eva Permaul e...@georgetown.edu Subject: [Histonet] Counterstain for dual chromogenic To: histonet@lists.utsouthwestern.edu Message-ID: 4da471ad.3040...@georgetown.edu Content-Type: text/plain; charset=ISO-8859-1; format=flowed Eva I have been doing

[Histonet] Re: Histonet Digest, Vol 89, Issue 9

We are needing to stain cardiac muscle for beta-actin. Anyone know of an antibody? most of the beta actin antibodies we have found say they do not stain cardiac muscle. Any suggestions greatly appreciated. Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended

[Histonet] Mouse cardiac myosin antibodies

I have been asked to stain mouse hearts for cardiac myosin. I have tried the antibody from Abcam (ab50967-Mouse monoclonal to heavy chain cardiac myosin) with no luck. I am using the Mouse on Mouse kit from Biocare and it worked well for me for some other mouse antibodies on mouse tissue but

[Histonet] IgG3 negative control

Can anyone tell me where I can buy mouse IgG3 to use as a negative control for some IHC I am doing. I have looked around in my usual places and can't find anyone who has it. Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of

[Histonet] Processing paraffin embedded samples for EM

We have been asked to take tissue that has been FFPE and deparafinize and process for EM. Has anyone done this? Any tips on what to do/not do? Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain

[Histonet] Re: Histonet Digest, Vol 80, Issue 37

Hi Mighnon Did you get a response to your request. I may be able to send you some. Please contact me at mark.elliott(at)hli.ubc.ca Mark Date: Fri, 30 Jul 2010 15:25:00 -0500 From: Mighnon Lashus mlas...@pathgroup.com Subject: [Histonet] Adenovirus controls To:

[Histonet] Myocardium infected with influenza

Does anyone have a block of myocardial tissue with a known influenza infection they can spare? Even a few slides would be great. Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information

[Histonet] Re: Histonet Digest, Vol 80, Issue 24

Hi everyone. I was asked the following question: I am just wondering if quantifying PAS staining (purple) is proportional to the amount of mucus produced from goblet cells? Thought I would ask the experts their opinion. Thanks Mark in sunny, warm Vancouver BC ***CONFIDENTIALITY NOTICE***

[Histonet] Region IX Education Event May 28 and 29th Vancouver BC

and there will be between 10-14 vendors present showing us their latest and greatest. Full details, including hotel information, can be found on the Region IX Website (www.nshregionix.org). Hope to see you there! If you have any questions please feel free to contact me. Mark Elliott Region IX Education

[Histonet] Deleting extraneous parts of posts when replying

Just a friendly reminder that if you are responding to a question to please delete out everything but the pertinent information, especially if you are receiving/responding in Digest mode. If you don't cut out the irrelevant posts it makes it extremely hard to find the new stuff. Some of the

[Histonet] Re MOM Kits

Thanks everyone for their input ion MOM kits. I have passed on the information to the person who asked me about them. Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the use of the addressee and may contain information that is privileged and

[Histonet] MOM Kits

Hi All Someone has asked me about MOM kists and since I don't do mouse work I thought I would ask the experts. What is the best MOM kit available for use with FFPE mouse tisseus? Thanks Mark ***CONFIDENTIALITY NOTICE*** This electronic message and any attachments are intended only for the

[Histonet] MMP-2 antibodies

I am looking for some advice as to which MMP-2 antibody people recommend for staining human tissue-either FFPE or frozen sections. I have tried one from Novus as well as Serotec with no luck. I looked in the Archives but couldn't find any. Any suggestions greatly appreciated. Mark abi jag

[Histonet] M! and M2 macrophages

I have been asked if there are antibodies/markers available for M1 and M2 macrophages that would work on human tissue, either frozen or fixed. Anyone know of any. I checked Biocompare and came up blank. Thanks Mark M1 macrophage A macrophage subtype that produces pro-inflammatory cytokines

[Histonet] CD8 or CD4 non Mouse primary

Hi everyone. We are needing to do a triple stain for IF using MHC Class II and CD4 and CD8. I have Mouse monoclonals to CD4 and CD8 and we have a goat anti-MHC. We were wondering if anyone knows of a good CD4 or CD 8 that is made in another species that we could use to avoid using two mouse

[Histonet] Region IX Education Day Toronto June 2009

Hi everyone. I forgot to mention in my earlier post about our upcoming Education Day that all the prices quoted are in Canadian dollars, so right now you get a great deal as the Canadian dollar has dropped in value against the US dollar. Mark Elliott Region IX Education Committee Chair

[Histonet] Region IX Education Day

you there! Mark Elliott Region IX Education Chair ***CONFIDENTIALITY NOTICE*** This electronic message is intended only for the use of the addressee and may contain information that is privileged and confidential. Any dissemination, distribution or copying of this communication

[Histonet] Antibody advice

Good morning everyone We are starting a rather large project and I would like to get some input as to the best version of antibodies to the following antigens-what are people using for the following and where do you get them from. This will be either frozen sections or formalin fixed paraffin

[Histonet] PRRX1 antibody from Abcam

Hi Everyone I was wondering if anyone has tried using this antibody for IHC, either paraffin or frozen. Datasheet says it hasn't yet been tested for IHC, only Western Blots. Does anyone have an alternate to the above that works? Thanks Mark Elliott in sunny, cold Vancouver, BC-no fog today