[Histonet] high complexity testing

What is the general opinion of the level of complexity for the Mohs tech assisting in a procedure. This includes the embedding, cutting and staining of the samples only. NOt the inking and marking of margins. Thank you Mary F Benoit, MT(ASCP) The Pathology Laboratory Lake Charles, LA

[Histonet] edge effect on IHC

I have been aware of some edge effect on an occasional slide, but lately we noticed the effect is showing up in the area of the slide that is farthest from the labels. We use DAKO Link Autostainer and their pretreatment baths for everything. One case in particular we stained a large melanoma

[Histonet] Glyco-Fixx

Need help solving a fixation/processing problem.  We are a regional reference Pathology Lab and we recently began receiving GI biosies in Glyco-fix from Thermo Scientific.  Our standard processing for small biopsies is formalin 2 changes for 1hour and 1.5 hours; 70% for 15 minutes; 95% 2

[Histonet] Congo Red for Amyloid

Need help with Congo Red for Amyloid problem.  We have been using automated staining with Dako's Artisan and recently tried manual procedure with fresh reagents from Polyscientific kit with little to no staining on two commercially prepared controls and two different patients who have been sent

[Histonet] in regards to review of peripheral smears

In our lab, the hematology dept has a form that they present to the pathologist with a manual differential( if only an automated one was done originally) and CBC with abnormal results , and a list of check boxes indicating whether they agree with technologist interp or if a written consult is

[Histonet] inverted meniscus

Today while perfroming an xylene purity check after recyling xylene on a CBG recycler, I noticed that the meniscus  between the DH2O and Xylene is inverted...that is convex instead of concave.  I have done this check many times( you add exactly 15 mls of water to exactly 85 mls xylene, invert

[Histonet] Estrogen and Progesterone Receptors

We currently are using Dako antibodies for Estrogen and Progesterone Receptors then followed with ACIS imaging on all infiltrating breast carcinomas.  We generally put a positive control on the bottom of the same slide for each to save a little time and money.  Results are great . Fixation is

[Histonet] Estrogen Receptor and Progesterone Receptors

Sorry, I forgot to sign my name. Mary F Benoit MT(ASCP) The Pathology Laboratory 830 Bayou Pines Drive Lake Charles, La   ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] fat clearing reagent

Our home made recipe is :  1925 mls of 95% alcohol  + 360 mls DH20 + 250 mls of Formaldehyde + 125 mls Glacial Acetic Acid.  Got this 20 years ago from one of my pathologists' journals and it works really well.  Let sit overnight and even the smallest nodes will turn opaque against a somewhat

[Histonet] PCR testing for Mycobacterium

Richard, Focus Diagnostics is a Reference Lab that tests for Mycobacterium and Cat Scratch. Focus Diagnostics, Inc.  5785 Corporate Avenue,   Cypress, California  90630 test # 51183    (800) 445-4032   Mary F Benoit The Pathology Laboratory 830 Bayou Pines Drive West Lake Charles, La  70601