Re: [Histonet] Fatty Fixation

2013-06-20 Thread Massimo
You can fix in Flemming's Liquid or, better in an aqueous solution of Osmic Acid at 1% for 24 hours. The Osmic acid has also the property to blacken fats. Kind Regards, Massimo Tosi Da: Rene J Buesa rjbu...@yahoo.com A: White, Lisa M. lisa.whi...@va.gov

Re: [Histonet] Gomori Trichrome Staining

2013-05-15 Thread Massimo
  with the Masson's trichrome staining: Cytoplasms from bright red to greenish. Kind Regards Massimo Tosi A humble Chemical Engineer who loves Histology Da: Eleonora Guadagnin guadagnin.eleon...@gmail.com A: histonet@lists.utsouthwestern.edu Inviato: Martedì 14 Maggio 2013 21

Re: [Histonet] Cheap digital camera microscope adapters?

2013-03-23 Thread Massimo
. Kind Regards, Massimo Tosi A humble Chemical Engineer who loves Histology Da: Rene J Buesa rjbu...@yahoo.com A: Mikael Niku mikael.n...@helsinki.fi; histonet@lists.utsouthwestern.edu histonet@lists.utsouthwestern.edu Inviato: Venerdì 22 Marzo 2013 14:50 Oggetto

Re: [Histonet] Mallory triple stain

2013-03-23 Thread Massimo
from the A Text-book of Histology, please see at: http://www.mediafire.com/view/?5gjm10epdg303ja Kind Regards, Massimo Tosi A humble Chemical Engineer who loves Histology Da: Rene J Buesa rjbu...@yahoo.com A: pru...@ihctech.net pru...@ihctech.net; histonet

Re: [Histonet] Fixation

2013-03-14 Thread Massimo
-to-better-histology-a-practical-guide-to-good-histology-practice/ I don't know if such procedure is suitable for the study of your specimen but take it just like a suggestion. My Best. Massimo Tosi A humble Chemical Engineer who loves Histology Da: Giulia Zunino

Re: [Histonet] microscope camera

2013-03-10 Thread Massimo
and you can still find something on eBay. Despite its low cost they give good results. Kind Regards Massimo Tosi A humble Chemical Engineer who loves Histology Da: Salomao Segal sseg...@slu.edu A: histonet@lists.utsouthwestern.edu Inviato: Sabato 9 Marzo 2013 20

Re: [Histonet] Chromic Acid for Cleaning Glass

2013-02-15 Thread Massimo
a slice to detach from a slide through a process. Massimo Tosi A humble Chemical Engineer who loves Histology Da: Pablo Sanchez-Quinteiro pablo.sanc...@usc.es A: Histonet@lists.utsouthwestern.edu Inviato: Giovedì 14 Febbraio 2013 10:36 Oggetto: [Histonet] Chromic

Re: [Histonet] Chromic Acid for Cleaning Glass

2013-02-14 Thread Massimo
only the side of the slide where I'll attach the specimen. Massimo Tosi A humble Chemical Engineer who loves Histology Da: Pablo Sanchez-Quinteiro pablo.sanc...@usc.es A: Histonet@lists.utsouthwestern.edu Inviato: Giovedì 14 Febbraio 2013 10:36 Oggetto: [Histonet

[Histonet] Histonet hey

2013-01-30 Thread Massimo
hey have a look http://bit.ly/WAOQgM Massimo ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Histonet hey

2013-01-30 Thread Massimo
hey have a look http://bit.ly/WAOQgM Massimo ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] Regarding a problem in manual paraffin embedding

2013-01-01 Thread Massimo
the pieces too hard. I use an intermediate mix of 50% 95° and 50% 100° ethanol solution too. It is advisable to make two or more steps in new ethanol and Xylene solutions. Kind Regards and wishing for a Happy New Year Massimo Tosi A humble Chemical Engineer who loves Histology

Re: [Histonet] Trichrome

2012-12-05 Thread Massimo
staining by hand. Best Regards, Massimo Tosi A humble Chemical Engineer who loves Histology Da: Cheryl tkngfl...@yahoo.com A: histonet@lists.utsouthwestern.edu Inviato: Martedì 4 Dicembre 2012 14:18 Oggetto: [Histonet] Trichrome Hi- need help troubleshooting

Re: [Histonet] Microtome knives

2012-11-11 Thread Massimo
, to be liable to less vibrations. Kind Regards, Massimo Tosi A humble Chemical Engineer who loves Histology Da: Jon Krupp jkr...@deltacollege.edu A: Histonet@lists.utsouthwestern.edu Inviato: Venerdì 9 Novembre 2012 19:49 Oggetto: [Histonet] Microtome knives

Re: [Histonet] Paraffin and Tissue

2012-09-28 Thread Massimo
it is advisable to repeat quickly the inclusion in a new paraffin. NOTE: I perform all the procedure in manual mode and not automatically. Kind Regards, Massimo Tosi -- A humble Chemical Engineer histlogist. Da: Demetria Ross deshsmi

[Histonet] To Jim Burchette

2012-06-23 Thread Massimo
! Massimo Tosi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Histological mordant

2012-01-05 Thread Massimo
have to filter the solution. I wonder if there will be a  leak as mordant or it will be necessary to lengthen the processing time. Any experience about? Thank you in advance.   Kind Regards, Massimo Tosi ___ Histonet mailing list Histonet

[Histonet] Merry Xmas

2011-12-24 Thread Massimo
To all my most sincere wishes of Merry Christmas, Massimo ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] paraffin temperature

2011-11-07 Thread Massimo
the water and you read the temperature at which the paraffin,  contained in the capillary , melts. Best Regards, Massimo Tosi Da: Amber McKenzie amber.mcken...@gastrodocs.net A: Jennifer MacDonald jmacdon...@mtsac.edu; Zoe rosa gaviotalopez...@hotmail.com Cc: histonet

Re: [Histonet] Eosin

2010-10-19 Thread Massimo
Yes. Especially when you watch into a test tube an 1% eosin solution and it is going to be diluted with distilled water. At that point it can be used for the staining of histological slices. Best Regards, Massimo Da: Rene J Buesa rjbu...@yahoo.com

[Histonet] How long ...

2010-09-19 Thread Massimo
in advance. Kind Regards, Massimo Tosi ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] How long ...

2010-09-19 Thread Massimo
. Kind Regards, Massimo ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

R: [Histonet] honing compound

2010-01-31 Thread Massimo
and thinest grid up to 1 micron. Best Regards, Massimo Tosi = In ricordo di Nice: E Argo, che aveva visto Odisseo dopo vent’anni, fu preso dal Fato della nera morte. Omero, Odissea, XVII, 290-327 --- Ven 29/1/10, Jennifer MacDonald jmacdon

[Histonet] (Used microtome) - Thanks ...

2010-01-13 Thread Massimo
... to all those who kindly responded at my email. Best Regards, Massimo Tosi = In ricordo di Nice: E Argo, che aveva visto Odisseo dopo vent’anni, fu preso dal Fato della nera morte. Omero, Odissea, XVII, 290-327

[Histonet] Used microtome

2010-01-11 Thread Massimo
Hi all, I am an amateur naturalist, and I'm looking for an used microtome, but working, even on old model, to be used for histological sections of samples included in paraffin. Please can anyone give me useful directions where to find it? Thank you all for you help, Massimo Tosi

R: [Histonet] Simple (?) equation question

2008-10-09 Thread Massimo
.) ---  8 gr. of Formic Ac.   Total solution = 5.4 + 9.1 = 14.5 ml. So I have to add 100 – 14.5 = 85.5 ml. of H2O   Best Regards,   Massimo --- Mer 8/10/08, jstaruk [EMAIL PROTECTED] ha scritto: Da: jstaruk [EMAIL PROTECTED] Oggetto: [Histonet] Simple (?) equation question A: histonet