When we switched on isopropanol as dehydratant and mineral
oil as clearant we forgot all our problem with processing
for fatty tissues and many other difficult type of tissues.
Thickness no more than 3 mm is main requirement for
processing such type tissues.
If you need details, please let me know.
SARAH:
If you will determine endpoint of
decalcification with any method and then
you will can be able to avoid similar problems.
Maxim Peshkov
Russia,
Taganrog.
You wrote at Friday, October 03, 2008 12:34 AM
> Does anyone have any ideas on the use of softener on
> decal blocks. Doesv this help 's
Carl:
In mineral oil all tissues will "clear" as in old days,
when we used xylene. This is good test for adequate
dehydration and clearing I uses now.
Mineral oil very well mixes as with IPA at 50oC,
as with paraffin at 60oC or so.
Regards,
Maxim Peshkov
Russia,
Taganrog.
Carl Hobbs <[EMAIL PROTE
Amos:
One disadvantage for manual processing with IPA is a little
odor of "cat's urine". This is can fully eliminated when using VIP
processor.
We changed ethanol on IPA more than years ago and happy with it.
We also uses mineral oil instead xylene and also happy.
However none of our techs want not
Dorothy:
We does Highman's method (Bancroft&Stevens, 1977 p.164)
Both 8 and 10 microns give good results.
Maxim Peshkov
Russia,
Taganrog.
mailto:[EMAIL PROTECTED]
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