[Histonet] sections falling off

in paraffin. Please help ! thanks megha -- Megha Kumar, Ph.D. Post-doctoral Research Scholar Sen-Bandyopadhyay Lab Department of Biological Sciences and Bioengineering Indian Institute of Technology Kanpur Kanpur, 208016 India phone - 9695830033

[Histonet] sections falling off in day 2 ISH

Hello everyone! When I subject murine skin and intestine paraffin sections (7-10microns) to ISH protocol, the sections fall off on day 2 when I add the SSC. The sections are taken on ploy lysine coated slides and remain on the slides when I do other protocols such as antibody staining. Can anyone

[Histonet] help ! paraffin section

Hi All I am trying to section adult mouse intestine and skin using paraffin embedding. However, when i section, the tissue is torn although the rest of the paraffin looks perfect. Please suggest why this is happening. Also, sometimes the skin sections fall off the slides when I perform in situ