Hello Susan,
Before I can help, I need more information from you. Are your samples fixed or
unfixed? In any case, make sure that the vibratome
is in working order & that all the movable parts are tight before you section
on the vibratome. In addition, during cutting the holding tray
should ha
Can you be more specific & tell us what DW means & please give us the protocol
for the sucrose including
the amount of DW used. This way we can help you.
Regards
Maria Mejia
Histology Supervisor
Department of Neurosurgery
UCSF
San Francisco, CA
From: his
Hello Jeff,
Let's see you are sectioning your (fixed) rat brain on the cryostat at 10ums.
I'm assuming they are mounted on standard size superfrost
plus slides & since they are fixed sections & even if they were not fixed -
after sectioning why not air-dry these sections standing up under
a f
Brett,
We mostly work & do IHC on 40um free-floating brain sections. These sections
were cut from fixed brain blocks. Sometimes, not on
a regular basis, but sometimes we have had to place our sections in (plain) PBS
solution & stored at 4C - overnight before continuing the IHC
protocol with no
Hello,
Here in our lab we section lots of rat & primate (large blocks) of frozen brain
tissues. We cut them at 40um
as free-floating sections & place each section in wells - using the 24 plate
well. The wells have 2ml each of
anti-freeze solution which is stored at 4C or -20C for IHC methods.
This is for anyone's interest who work with 40um primate brain section 2x3
inches. We routinely
mount our IHC stained sections from working PBS on pre-subbed brain slides from
Brain Research
Labs.
To keep the sections on the slides we use a very fine (#0) brush to brush the
section flat on th
