Can you be more specific & tell us what DW means & please give us the protocol for the sucrose including the amount of DW used. This way we can help you.
Regards Maria Mejia Histology Supervisor Department of Neurosurgery UCSF San Francisco, CA ________________________________________ From: histonet-boun...@lists.utsouthwestern.edu [histonet-boun...@lists.utsouthwestern.edu] On Behalf Of vaigunda ragavendran [rags_...@yahoo.co.in] Sent: Friday, August 20, 2010 1:19 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Re: Cryoprotection Hii, If instead of sucrose in PBS, we use sucrose in DW by mistake, what might happen to fixed tissue sections during cryoprotection.. Please lemme know.. Rags, Postdoctoral Research Fellow, Verge Lab, Department of Anatomy and Cell Biology, Room No: 5800, Saskatoon City Hospital, 701, Queen Street, Saskatoon, Saskatchewan S7K 0M7, Canada. Phone (Lab): +1 306-655-8714 (Facsimile): +1 306-655-8709 _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet