[Histonet] Alkaline phosphatase staining in FFPE tissue

2015-02-25 Thread Melanie Smith
Hello, I was wondering if anyone has had success in staining osteoblasts via alkaline phosphatase in decaled ffpe tissues? Currently using a sigma kit without success even with pre-incubating overnight with magnesium chloride. Thanks! Melanie -- Melanie Smith, MS University of Delaware melsm

[Histonet] TRAP staining FFPE sections

2015-02-05 Thread Melanie Smith
of contrast due to the yellow that I'd like to improve. Thanks! -- Melanie Smith, MS melsm...@udel.edu ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Mouse FFPE IHC double stain caspase-3 and Ki67

2014-10-08 Thread Melanie Smith
incubation. If anyone has any insight into caspase-3 antibodies or doing IHC double staining that would be great! Many thanks, Melanie -- Melanie Smith, MS Research Technician II Department of Biomedical Engineering University of Delaware melsm...@udel.edu