Re: [Histonet] What is the best microtome?

2017-09-14 Thread Dr. Michael Gudo via Histonet
We have Leica microtomes in out lab and they work very very good. The components are compatible over several Generations and they work for at least more than 15-20 years! And the best thing is the Waterbath directly attached to the Blade. Greetings Michael Von meinem iPhone gesendet > Am

Re: [Histonet] Paraformaldehyde Fixed Tissue

2017-08-29 Thread Michael Gudo via Histonet
Dear Sandra, Dear Ana, in our lab for tissues for which we have enough time for infiltration we fix for at least 48 hours or more in NBF or PFA (both with 4 % reactive formaldehyde, which is equivalent to 10% „neutral buffered formaline“) and then first wash in tap water for a minimum of 1-2

Re: [Histonet] Cut FFPE Slides for ISH

2017-08-29 Thread Michael Gudo via Histonet
Hello Jenniffer, we store the slides with section at 4-8 °C for many month or even longer and we do not have any problems with ISH. Best regards Michael > Am 28.08.2017 um 17:30 schrieb Jennifer Phinney via Histonet > : > > Hello Histonet, > I am hoping