[Histonet] Cryostat to room temperature

Does anyone know how long it would take a cryostat that is maintained at approx. -22 degrees C to come to room temperature after being turned off? Any thoughts are appreciated. Barb Moe Histology Lab Gundersen Health Systems La Crosse WI

[Histonet] DAKO Artisan special stain kit validations

For those that are using the DAKO Artisan for special stains could you comment on how you are handling implementing new kits? When a new lot number on a kit is received do you run a positive AND negative control either prior to use or concurrent with a patient case? Any thoughts are

[Histonet] PPE for frozen sections

Could anyone share what PPE you are required to wear when performing a frozen section? If doing a frozen section on a piece of lung or mediastinal nodes are you required to wear a respirator such as N95 or even a PAP-R? Any thoughts are appreciated. Barb Moe Histology Lab Gundersen Health

[Histonet] ER/PR controls

For anyone making in-house ER/PR controls could you comment on what tissue you have in your control block -- one piece of positive tumor regardless of reactivity strength 1+, 2+, 3+ (weak, medium, strong) or multiple pieces to represent each level of reactivity? Also, does anyone order

[Histonet] Control tissue

Could anyone share their process of finding and validating control tissues for IHC? We currently use tonsil tissue as control tissue for 23 different antibodies for IHC (CD2, CD3, CD4, etc.). My current process is to have our grossing personnel submit some extra tissue from a surgical case.

Re: [Histonet] Performing PAS with Diastase by Hand

Jim - We use amylase from porcine pancreas, from Sigma. Barb Moe Gundersen Health System La Crosse WI From: Ingles Claire via Histonet [histonet@lists.utsouthwestern.edu] Sent: Wednesday, October 05, 2016 5:06 PM To: Vickroy, James;

[Histonet] Kidney bx transport solution

Hi all - Currently our histology lab staff travels to our Interventional Radiology suite when a kidney bx is performed. At the procedure site, the sample is handed directly to the tech, it is evaluated for adequacy, divided, and put into the following solutions - formalin, Trump's, and Zeus

[Histonet] B-5 fixative

Can anyone tell me why B-5 fixative is called B-5? Does the B stand for something, or does the 5 have a meaning? Any thoughts are greatly appreciated! Barb Moe Histology Lab Gundersen Health System La Crosse WI ba...@gundersenhealth.orgmailto:ba...@gundersenhealth.org

[Histonet] Amyloid control tissue

Does anyone know where I could purchase (or trade for) a block of amyloid positive tissue? Alternatively, for those who purchase ready-cut amyloid control slides, could you share what vendor you have been happy with? Thanks in advance! Barb Moe Gundersen Lutheran Medical Center La