https://oag.ca.gov/sites/all/files/agweb/pdfs/cci/safety/picric.pdf
Two hazards: formaldehyde and picric acid (see attached).
While I agree with Dr. Richmond, changing protocols is not easily done.
So. You may search for appropriate oxidizers of aldehydes. OR!!! You may
take the reasonable
And I, "O>" scary one, is following your class by leaving 38 days BEFORE I
will be 78.
You really are old!?""@
In my family, retirement has appeared to lead directly - one way or another -
to demise, and I have yet to discover anyone who has turned the inevitability
of that ending. So, I
And, I always rinsed the Schiff with 'absolute' HOH before the tap water rinse
- which rinses were taken from my protocol for Ehrlich's 'Acid' Hematoxylin
that was mixed from scratch and aged in the second story deep window sills on
the West wall of the Biology/Geology building at Lehigh
Have no expertise other with 'library.'
So, here is some new stuff that I found.
http://www.autoqbiosciences.com/products/3d-laser-nanodissection-systems/tissue-surgeon/
Back in the 1960's when I was learning all about the PAS method, I got my hands
on a new CRYO-Stat (-30_!!!) and tried my 'stuff' on fresh frozen
sections. Having been in the habit of controlling every variable I could name,
I included a 'Schiff' control with each PAS.
Halloween story of a 'tech' who lacked a certificate.
T'was a Sunday, and he, a student at the age of 24, was "ON" for the weekend
covering blood bank, chemistry, and hematology. A call came in for a unit of
blood for a Friday surgical - just to bring the patient up to speed. He, for
reasons
He refused to send a unit without an order for a new cross match, which he got
with some argument. Only 4 of the 9 previously good units passed as he
re-crossed all ten, and the one he sent did not pass the second crossing. He
called the pathologist on duty, who told him to give up going to
I slways filtered my air (5um) and my water (0.22um). Only tap water rinses
for Hemnatoxylin, Schiff's and other rinses were not rinsed. All of my MolBio
and Immuno- stuff was done with filtered (clean?) water at least.
The rule is: be Monkish at the bench and as messy as you like in your
Long ago, in the foggy, foggy past, we were admonished to insure that our
Eosin in the bottle had a slight 'green' tinge in good daylight.
When we finally got fluorescence, we would check an HE-stained section with
the fluorescein filters to insure the fluorescence of the Eosin.
Very
A strongly recommended first interview question: How many digital cameras do
you have with you today?
Cheers,
FCM
Frederick C. Monson, PhD
Technical Director
Center for Microanalysis and Imaging, Research and Training (CMIRT)
West Chester University of Pennsylvania
Schmucker Science South -
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