I have two open positions at Texas Childrens Hospital.
There is one in the IHC lab and one in Special Stains.
If interested apply at Texaschildrenshosptial.org
Thanks,
Felton L. Nails
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Was just wondering how everyone is handling the new changes to IHC 88342 and
the use of 88341?
Thanks.
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privileged. If you are not the
My opinion is that it is a good idea because many hospitals are shutting down
their EM department because of a lack of skilled workers.
So the more skills they have the better for them.
Let me know if any of your students want to move to Houston Texas.
Felton Nails
Texas Children's Hospital
I always tell my students if you can cut, you can get a job. It appears that
you school did not properly prepare you for the demands of an average histology
job.
You need to take every opportunity to work on your craft and the major focus of
histology is cutting. With 6 to 7 years of experience
Has anyone started scanning requisitions and discarding the original AP reqs?
If so does this meet the CAP retention requirement?
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Are the blocks that are not being sent cracking?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, February 06, 2014 8:08 AM
To: Erica Canal; histonet@lists.utsouthwestern.edu
Are the basic muscle and nerve biopsy stains being performed manually or has
someone automated these stains?
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privileged. If you are not
If you are referring to the IPS slide writer, I would say continue looking it
is very unreliable.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Catherine
Simonson
Sent: Monday, October 21, 2013 1:15
When you have a patient that expires, do you require that the bodies be
brought down in body bags for an autopsy or funeral home pick up?
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Any ideas in histoland where I can find control tissue for BK Virus IHC?
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9-26-2013
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I have even tried copying the following link
(histonet@lists.utsouthwestern.edu) and pasting it and I'm still unable to make
a post.
-Original Message-
From: nancy lowen [mailto:clayca...@yahoo.com]
Sent: Wednesday, September 25, 2013 10:49 AM
To: Nails, Felton; 'Joanne Clark
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are hereby notified
test
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I am in the same situation???
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joanne Clark
Sent: Tuesday, September 24, 2013 12:54 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Submitting
We have been using recycled xylene for years and have found that it only
present a problem on the clean cycle of the processor.
Everywhere we have not experienced a problem, so we use new xylene on the
cleaning cycle.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
I have heard of both methods, currently we put double ribbons on one slide.
The only time we have repeats is if the initial cut is superficial.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce
I would like to be included on this request also, We currently have the DAKO
special stainer and are looking to see what else is available.
The DAKO unit is very good but we are being forced to sign a reagent agreement
with a minimum commitment even though we own the unit.
-Original
What system are you using to place these orders in the OR?
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Weems, Joyce K.
Sent: Tuesday, March 26, 2013 2:27 PM
To: 'Duddey, Aimee';
Before you holler political and blame the candidates, ask yourself who was hurt
most by POL's?
Large reference labs.
With this change they will get back the business because it will not be
profitable to establish a POL.
Also they lobbied for and increase on the PC, reference have pathologist,
Let's face it POL's were established to make more money for the groups, which
takes money from the big reference labs who have large lobbyist groups.
HUM, I wonder who maybe driving these efforts.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Can you list the article, because I to would be interested.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Thursday, October 25, 2012 10:00 AM
To: Will Chappell; Hannen, Valerie
Cc:
What was the initial purpose for starting the Histonet, I assume it was to
offer a forum for Histotech to get assistance with problems they may encounter.
It offends me when there are those that feel they can control what is posted,
the histonet is for the group not a few individuals.
Histocare
Try using histoscreen cassettes and that will eliminate the need for wrapping
the tissue.
If still insist on wrapping, ensure that they are dipping the tissue paper in
formalin prior to putting the tissue on the paper.
Also at embedding, if the tissue is stuck to the paper, take a blade and put
I would have to agree with Rene. I have had two VIP's that were replaced after
they were over 20 years old and they both were still running. I currently have
3 VIP 6's, thus far the only problem I've experienced was the fluid level
sensor malfunctioned once.
-Original Message-
From:
I studies at the famed AFIP while in the military and have two degrees and
never thought I would stay in histology. 22 years later I am still in the field.
I don't think OJT is a bad route it has often generated some of our best techs
but I am a firm believer that new techs need to go through a
This is a very sticky issue, when I setup inhouse labs I always present the
argument that during inspections the lab that produces a report should have
access to all test material which includes blocks and slides per the CAP
checklist.
Ann trying approaching it from that angle.
-Original
@lists.utsouthwestern.edu; Nails, Felton
Subject: RE: [Histonet] Block Storage
Depending on the state regulations and the laboratory policies,blocks are
stored during different periods of time before being discarded. I used to store
them for 9 years.
René J.
--- On Wed, 3/21/12, Nails, Felton flna
This is an issue with the cassettes not your printer or ribbons.
If you take a straight edge and put it up against the edge of the cassette you
will see that he cassette is concaving causing the styles to loose contact with
the cassette.
It is more of a problem at the middle top edge of the
I agree with Joe, Mopec makes a very good product. In the last 4 years I have
purchased 6 MB6000 and our pathologist are very pleased with them, especially
the absence of high noise levels and adjustability.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
This sounds like union rules not CAP rules
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Wednesday, December 07, 2011 9:55 AM
To: histonet@lists.utsouthwestern.edu;
The patient condition should dictate what is taken, so the number will vary.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of SHANE NELSON
Sent: Friday, November 04, 2011 6:12 AM
To: Histonet
Subject:
I want to poll Leica Bond III users to see if they are able to successfully run
EBER Insitu on their unit?
We continue to get inconsistent staining and inconsistent background staining.
I have been told it's a pH problem, it's a tissue processing problem but we are
able to do the stain with
I purchased a new Microm HM525 cryostat about 3 months ago.
The unit overheated causing some of the internal parts to warp.
Has anyone else experienced this or similar issue?
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We have been using the Bond III for about 4 months and have starting
experiencing staining on our negative control slide.
We inserted and additional blocking solution and it help for the IHC stains but
we are still experiencing the problem the with insitu.
Has anyone experienced this problem
I use a clipped corner slide from Statlab and we do not experience any
problems.
(IJL-6110)
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of karlisch,
patricia
Sent: Wednesday, July 06, 2011 10:32 AM
If you are using one of the older film coverslippers there is a roller
following the application of the film.
Make sure that roller is still in the correct place or that one of the springs
is not missing or in need of replacing.
The job of that roller is to squeeze the air bubbles from under the
Your best answer will come from ASCP so contact them directly. The number
should be on the application
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Eric Velazquez
Sent: Wednesday, May 04, 2011 4:37 PM
We switched from the gross lab senior because of premature rusting, with the
Sakura demo our pathologist thought it was too loud.
We selected the Mopec MB600 and they have been very pleased.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
100% is unrealistic unless your staff never calls in sick, take vacations, your
lab never gets research projects that are needed today and you are always fully
staffed. Realistic reside between 90 and 95% which is obtainable but not overly
easily obtainable
-Original Message-
From:
We have experienced similar issues, however you can just call DAKO and they
usually will replace it.
It is important that you call them so they will be aware of the problem and can
pull that lot # if needed.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
I am looking for a reliable method for staining mouse muscle with ATP'ase pH
4.3, 4.6 and 9.4.
Any help will be greatly appreciated.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Tom McNemar
Sent:
I thought I was the only histology lab that got stuck staining bone marrow
smears.
I started with staining smears manually, then went to the hematek and finally
discovered the Wescor stainer. The wescor stainer has really brought
consistency to this area. The only potential problem is you have
I think I recall that statlab or mercedes medical carried that item.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kaye Ryan
Sent: Tuesday, January 18, 2011 11:20 AM
To:
As have you, I have also had great service from my VIP's and plan on purchasing
the VIP 6.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Parker, Helayne
Sent: Monday, December 27, 2010 3:16 PM
To:
The problem with the Nexus is that you can not do silver and AFB on the same
run.
We use the Dako artisan with very consistent results and it is very easy to
use.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On
Is anyone perform immunofluorescence for C3D?
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I agree most of your times are too long, but you can still get sections if you
put water on your ice block and allow the tissue to rehydrate a bit or put a
little ammonium hydroxide and water on your ice and sit your blocks to be
section in this solution.
-Original Message-
From:
I have had some experience with this company and their embedding station but
not microtome. The small cold area where you orient the tissue tends to ice
over.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On
We use marking ink from Statlab or Mercedes Medical.
After applying the ink to the tissue, we take weak acetic acid solution and
blot it over the ink which helps it adhere.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
these little
quistions. and i asked befor for name of antibodies and its use to be written
to clear the information for all levels and no shame for that.
--- On Thu, 9/16/10, Nails, Felton flna...@texaschildrens.org wrote:
From: Nails, Felton flna...@texaschildrens.org
Subject: [Histonet] RE: Cutting
what is happening to our field??
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Senn, Amy R
Sent: Thursday, September 16, 2010 10:38 AM
To: Histonet@lists.utsouthwestern.edu
Subject:
That is the case here also
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mahoney,Janice A
Sent: Wednesday, September 08, 2010 10:37 AM
To: 'Weems, Joyce'; Demarinis, Carolyn;
Even over Tissue Tec
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Madary, Joseph
Sent: Thursday, September 02, 2010 12:32 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] embedding center
We have always done IF's utilizing internal positive controls. The new CAP
checklist is now requiring positive and negative controls. What are you guys
doing for negative controls?
CONFIDENTIALITY NOTICE:
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Tonsils are gross only unless there is a difference of 5cm between the two
tonsils.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
Sent: Tuesday, August 31, 2010 12:50 PM
To: Histonet
It's not the oil that's giving you trouble because they have always had oil
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Brandi Higgins
Sent: Monday, August 30, 2010 3:12 PM
To:
Agreed extremely reliable
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Sent: Friday, August 13, 2010 3:41 PM
To: histonet@lists.utsouthwestern.edu; Mike Tighe
Subject: Re: [Histonet]
Has anyone been experiencing a brown cornflaking artifact on their slides after
being coverslipped.
I was told by Sakura that it was do to the slides drying out when transferred
from the stainer to the coverslipper.
Which can't be correct because on the new sakura coverslipper there is a
Statlab or TBS
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Diana McCaig
Sent: Thursday, July 15, 2010 9:53 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] inking dyes
Can someone provide
Not yet
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Scott, Allison D
Sent: Thursday, July 01, 2010 2:37 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cap Question: 2 identifiers for
Concerning Immunofluorescence, where are you getting your (PBA) Protein
Blocking Agent from? I use to order it from Thermo Shandon but they no longer
carry it.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On
Would anyone out there in a Derm Lab be willing to share their procedure used
to process tissue?
From: histonet-boun...@lists.utsouthwestern.edu
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of thisis...@aol.com
[thisis...@aol.com]
Sent: Sunday,
If you are supplying the professional component, then it is a good idea to
maintain the blocks and slides.
If you are inspected and they do an audit trail you must be able to produce the
slides and/or blocks.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
...@lists.utsouthwestern.edu] On Behalf Of Nails, Felton
Sent: Friday, April 30, 2010 7:12 AM
To: 'Daniel Adams'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Slide and Block Storage
If you are supplying the professional component, then it is a good idea to
maintain the blocks and slides
So what if the reporting company need to order special stains, it adds too much
time having to request the blocks or additional unstained slides.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Elizabeth
It really depends on your volume to be processed and what you are planning to
process.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Urim, Lyudmila
Sent: Monday, April 26, 2010 10:10 AM
To:
What do you process and don't measure, so everything is grossing. Even our
foreskins which are GDO, we take a measurement in our dictation.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathleen Boozer
There is about three brands of paraplast and they all cut differently. In one
of my labs I use paraplast plus and it ribbons very well however the blocks
have to be colder then if you are using TissuePrep from Fisher.
Leica may have sent you one of the other types of paraplast. (paraplast,
It depends on your volume and how long you maintain your slides. Because you
are a children's hospital you keep them longer.
The Leica brand is very troublesome in high volume situations. I personally
prefer Sakura tape for the speed and fast drying time.
-Original Message-
From:
Is this coverslipper now produced by Medite
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of jstaruk
Sent: Thursday, March 25, 2010 2:40 PM
To: malbena...@gmail.com; 'Lynette Pavelich'
Cc:
Basically time
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patsy Ruegg
Sent: Tuesday, March 23, 2010 11:00 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] help
After you stop laughing
Why would you want to prelabel the containers prior to seeing the patient?
Outside of the kit being labeled LLB LLM LLA, etc., the individual containers
should not be label until you actually add the sample to avoid mistakes.
-Original Message-
From:
It is my understand that the two patient identifier applies to the completed
slide not the block.
You can verify this by looking at the CAP Checklist for anatomical pathology
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Joe, I wanted to ask you a question about which type of IHC stainer you are
using and which stainer do you think is the user friendly and efficient.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joe
Is there a good Sirius Red procedure used to stain collagen
And where can I find the powder
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Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
Thanks for everyone's help
From: John Kiernan [mailto:jkier...@uwo.ca]
Sent: Wednesday, February 24, 2010 11:36 AM
To: Nails, Felton
Cc: histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] Sirius Red
You'll find brief instructions for the picro-sirius red
Thanks Beth, I have not had a lot feedback on this question.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Beth Millerman
Sent: Monday, February 15, 2010 1:19 PM
To: histonet@lists.utsouthwestern.edu
Have anyone in Histoland converted from 10% NBF to the Ultralight Histology
system of processing?
I think it is marketed by Pacific Southwest Lab Equipment
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf
I have been fighting this battle for years and still have not been successful.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Mike Pence
Sent: Thursday, February 11, 2010 8:44 AM
To: dkb...@chs.net;
Especially when these physician hire unqualified people to run these labs and
flood the histonet with their uneducated questions.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Richard Cartun
Sent:
Please forward me a copy.
Thanks,
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
kdwyer3...@aol.com
Sent: Saturday, January 30, 2010 12:23 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet]
Have you tried Tom Buck with Hawkeye biomedical
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cheri Miller
Sent: Thursday, January 21, 2010 12:05 PM
To: histonet
Subject: [Histonet] Reliable Bio repair/
Allison we use tissue prep paraffin and it comes in cardboard containers, we
save the empty containers to pour the waste paraffin in. Once it solidifies we
dump it in the biohazardous container to be disposed of.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
You have to infuse the lung with a 50/50 oct sucrose solution prior to
freezing.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Meghan Tucker
Sent: Monday, January 04, 2010 9:23 AM
To:
I have just purchased two Mopec MB600 grossing stations and we do not have this
problem. Our pathologist actually rave about the unit.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Kathy M. Gorham
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