Re: [Histonet] need tips for cross-sectioning of cortical bone

very mouse biology small lab, I do all the processing, cutting, and staining myself. So, what works for me may not be feasible for everyone, and may still not be the best way to do things (although I do try to generate beautiful data). Sincerely, Nicole Collette LLNL/ UC Berkeley At

Re: [Histonet] herring sperm dna

Hi, Emily, We use Salmon Testes DNA for our hyb buffer for whole mount RNA in situ on mouse and frog embryos, Sigma catalog # D-9156, comes in 5 x 1ml (11.0 mg/ml) tubes. It says "for hybridization" on the package ;) Hope that helps. Sincerely, Nicole Collette Lawrence Livermor

Re: [Histonet] Tris Buffer Preservative

k the contamination. However, I'm not in a high-throughput lab, so this works for me (I'm a one-pair-of-hands histology lab). Sincerely, Nicole Collette Lawrence Livermore National Lab/ UC Berkeley At 11:59 AM -0500 2/24/10, Amos Brooks wrote: Hi, I have been making up my own

[Histonet] autofluorescence experiment

treatment (and more washing) cannot or should not be used? I tried them together on my unstained slides, they look pretty darn fabulous. Just striving for clean data and beautiful pictures. Thanks again for all your help, Sincerely, Nicole Collette Lawrence Livermore Natio

[Histonet] shipping slides, elementary question

ging and labeling though...), but don't want to just send a giant box and have them all broken on the other end. Maybe there's another alternative? I'm sure someone on histonet has done this before :) Thanks in advance for the advice. Sincerely, Nicole Collette Lawrence Livermore Na

Re: [Histonet] cryojane tap transfer system

Temperature is also somewhat of a factor, I've found that I would section at colder temperature than I normally would for that tissue to get it to crosslink well- sounds counterintuitive, but there it is. This is purely based on my own trial and error, take it with a grain of salt and good

[Histonet] TRAP stain in bone

ork like Fast Garnet GBC, as a red stain?), and assorted acids and buffers if that makes a difference. I am happy to purchase additional reagents that will work. Thanks so much for your help and support! Sincerely, Nicole Collette Lawrence Livermore National Lab

[Histonet] IHC double labeling question

ieval is the same? Thanks for the advice! Sincerely, Nicole Collette LLNL/UCB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] So... I Need Some Expert Advice (forum for information):

7;s a lot of good info here, and some really great expertise, but finding it threaded through the archives is not the easiest way to find this stuff, and it is evident by the discussions that come up over and over. Thanks for listening! Keep up the good work! Sincerely, Nicole Collette _ At

[Histonet] IHC, decalcification preferences

vice from this listserv. Sincerely, Nicole Collette LLNL/UCB ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] hand processing schedule late mouse embryos

fantastic sections. I suspect it could be incomplete washing of the EDTA before infiltration, but it's possible that the processing schedule is just not long enough. Any advice? Thanks in advance! Happy Friday! Sincerely, Nicole Collette Lawrence Livermore National L

RE: [Histonet] IHC on paraformaldehyde-fixed

Nicole Collette LLNL postdoc Good point. I never tested it directly side-by-side to check for the effects of 1% methanol directly. It could be old-fashioned paranoia. However, it was a variable I wanted to omit as I know methanol (we are talking 100%) has deleterious effects to some mouse antigens

[Histonet] lipid preservation in paraffin and mineralization of tissues?

this protocol with (or without) success, I'd be grateful for the advice. Thanks in advance, Nicole Collette LLNL/UCB [EMAIL PROTECTED] ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet