Re: [Histonet] Histonet Digest, Vol 234, Issue 10

Aren't they going to offend the mail histotechs with that name? Just kidding. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 39037 N 11th Ave Phoenix, AZ 85086 C 720-281-5406 prueg...@hotmail.com Doug Ruegg C720-281-5407 douglas...@hotmail.com From:

Re: [Histonet] Histonet Digest, Vol 233, Issue 7

this is a strange post on histonet Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 39037 N 11th Ave Phoenix, AZ 85086 C 720-281-5406 prueg...@hotmail.com Doug Ruegg C720-281-5407 douglas...@hotmail.com From: histonet-requ...@lists.utsouthwestern.edu Sent:

Re: [Histonet] Histonet Digest, Vol 223, Issue 14

I used tonsil as + control. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 39037 N 11th Ave Phoenix, AZ 85086 C 720-281-5406 prueg...@hotmail.com Doug Ruegg C720-281-5407 douglas...@hotmail.com From: histonet-requ...@lists.utsouthwestern.edu Sent: Friday, June

Re: [Histonet] Histonet Digest, Vol 213, Issue 22

I would suggest using something like histogel or agar to make layers of blood drops in a tube, then freeze them and make frozen sections. Sounds tricky. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 39037 N 11th Ave Phoenix, AZ 85086 C 720-281-5406 prueg...@hotmail.com Doug Ruegg C720-281-5407

Re: [Histonet] Histonet Digest, Vol 211, Issue 7

The blue stain from prussian blue is a chemical reaction and indicates that there is a lot of iron in the section, you would not want it not to stain some of the iron there would you? I agree with John, take an adjacent section for a general stain. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC

Re: [Histonet] Water under sections

This is all good advise. I always picked up section and tapped the slide on counter and then dried standing up. This is really a problem if you are at all above 5K feet in altitude where water boils violently at 92-93dc, which will destroy your tissue section. I have written a couple of papers

Re: [Histonet] Microtome at home

I have done it, but you are right, I had my own private business, not sure why it would be a problem, especially for research. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com

Re: [Histonet] FW: Microtome at home

I agree with this point and as far as clocking in and out, I would think you could work out something like getting paid piece mill, perhaps charge per slide or block cut, that way you could do it on your own time and not have to clock in. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E

Re: [Histonet] Need a procedure

From: John Garratt Sent: Thursday, January 23, 2020 10:51 AM To: Terri Braud Cc: histonet@lists.utsouthwestern.edu Subject: Re: [Histonet] Need a procedure Hi Terri, I suggest you use Histogel for block preparation. It works exceptionally well, it is good

Re: [Histonet] Histonet Digest, Vol 195, Issue 10

I never minded being called in to assist for an aspect of transplants at the U, I figured I was helping a very needy patient. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com

Re: [Histonet] Need a procedure

Terri, when I was in animal research we used the blood from the animal to make plasma and combined it with some thrombin from the hospital pharmacy. Of course that is tedious especially since histogel is available. I warmed the histogel by placing a tube of it in a beaker of hot water, do not

Re: [Histonet] Frozen Gel Pack with paraffin block for shipping?

whether I used an ice pack or not, I always bagged each block in it's own individual container/plastic bag, etc., just in case there was melting the tissue could possibly be recovered. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C

Re: [Histonet] Histonet Digest, Vol 184, Issue 1

here here Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: Joseph Saby Sent: Tuesday, March 5, 2019 9:47 AM To: Jordan, Kelley; Mark Tarango Cc: HistoNet Subject: Re:

Re: [Histonet] Histonet Digest, Vol 184, Issue 1

Maybe that is the trouble, they keep passing these technical issues on to Marketing instead of R Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com ___ Histonet

Re: [Histonet] New York Qualifications for Histology Supervisor

In my opinion this issue is related to the fact that HT's are not required to have BS degrees, like Med Techs and Cytotechs, and that clia/cap does not require ASCP certification to work in a histology lab. We have always been the red headed step child in the lab because of this. This is why

Re: [Histonet] Bone PMMA sections

Stefano, after cutting the sections, dry them on a heat plate, then they can be stored dried stacked next to each other. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com

Re: [Histonet] Re guinea pig IHC

good advise Carl and you asked some of the things I was wondering. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: Hobbs, Carl Sent: Saturday, February 2, 2019 12:38

Re: [Histonet] guinea pig IHC

Especially a very blood tissue like GP spleen. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: Patsy Ruegg Sent: Thursday, January 31, 2019 11:51 AM To: Jan Shivers;

Re: [Histonet] guinea pig IHC

In my experience it is not that GP have a higher peroxidase level, it is frozen sections in general that cannot be blocked with h202, unless they are fixed for a long time in formalin. What are others experiences with h202 blocking on frozen sections. I always used an IHC detection system

Re: [Histonet] IDH1 source

Hi Richard, How are you? Dianova is a really good ab supplier, I used their CD31 for animal research and it was excellent. Happy Holidays. Patsy Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com

[Histonet] Fw: Announcing the 12th annual retreat of applied immunohistochemistry and molecular pathology at the beautiful Marriott, Key Largo, FL

Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C 720-281-5406 prueg...@hotmail.com From: ancillaryp...@mac.com Sent: Saturday, October 7, 2017 6:03 PM To: ihcrg ihcrg Subject:

Re: [Histonet] Survey!!!!!!

I have seen so many more problems with auto coverslippers, they break down, the cover does not hold up over time for archiving, etc., that I would would probably chose an autostainer. Patsy Ruegg, HT(ASCP)QIHC Ruegg IHC Consulting 40864 E Arkansas Ave Bennett, CO 80102 H 303-644-4538 C