Re: [Histonet] [EXTERNAL]TEST

Hi Terri, I see your message.  Have a good day. Jessica Jessica Piché, HT(ASCP) Histology Team Leader, Laboratory Waterbury Health 64 Robbins Street Waterbury, Connecticut, 06708 Phone: 203-573-7167 FAX: 203-573-7242 Email:jessica.pi...@wtbyhosp.org Waterbury HEALTH

[Histonet] Premade Eosin

Good Afternoon, I hope everyone is having a great week so far! Can anyone share what pre-made eosin brands they are using and recommend? We use Eosin Y. We make it ourselves and want to look into pre-made. Thank you in advance. Have a great day. Jessica Jessica Piche, HT(ASCP) Waterbury

Re: [Histonet] Question Regarding HM355S Automatic Microtome

Hi Diana, We are not currently using the HM355S in our lab, but we did demo it and were not fans. I would see if you can demo one to see if you like it. We had the Thermo Shandon Finesse and Finesse Me models and we loved them but the HM355S didn't appear to be designed the same way and we

Re: [Histonet] preordering giemsa stains

Hi Paula, We do H.pylori on all specimens related to stomach upfront. We also do Ab Pas too. I think it is fairly common in lots of labs. Have a good day. Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167

Re: [Histonet] Marking Pen

Hi Paula, What is the first solution the cassettes go in when the processor starts? We use Statmark pens when our cassette printer isn't working, and they work well. The only time we have had issues was when we would hand write the cassettes and run them on our small biopsy run which skips the

Re: [Histonet] Tisse disposal/formalin

, 2023 2:54 PM To: Piche, Jessica Cc: histonet@lists.utsouthwestern.edu Subject: RE: Tisse disposal/formalin You don't often get email from greg.dob...@gmail.com. Learn why this is important<https://aka.ms/LearnAboutSenderIdentification> [EXTERNAL MSG] Decant formalin off into formalin

[Histonet] Tissue disoposal/formalin

appreciate any information that you can share. Thank you, Jessica Piche Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

Re: [Histonet] Coffee at the desk

It also doesn't set a good impression for everyone else in the lab since they can't have a coffee at their stations. If it could be done, I wouldn't want everyone else's coffees on my desk either, but I'd have to because I'd want to make it fair to everyone. If only it was safe for all of

[Histonet] SMAD4 antibody

Good Morning and Happy Friday! Is anyone running Smad4 on the Dako Omnis? If so, would you be willing to share your protocol? We are having a hard time working this one up. Thank you and have a great day! Jessica Piche, HT(ASCP) Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory

[Histonet] Specimens for Gout

Good Morning Everyone, I hope you all had a nice weekend. What are people doing to process gout specimens? Are you fixing them in Absolute alcohol (and for how long), and then loading on tissue processor starting in xylene? I'm hearing different things and am curious about what other people

[Histonet] CAP regulatory question

Good Afternoon, Do we only have to keep lot to lot comparisons for immunohistochemistry and special stains for 2 years? I hope everyone is having a good day. Thank you. Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167

[Histonet] Thyroid FNA's and other FNA procedures

Good Morning, Who makes the slides for fine needle aspirates at your facility? What are the qualifications to make the slides? I apologize if this message posted twice. Thank you, Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory Histology Team Leader 203-573-7167

[Histonet] procedure manuals for special stains

Good Morning, I hope everyone is doing well. We have the Ventana Roche Benchmark special stainer. How is everyone doing their procedure manual for the actual stain protocols? What is the CAP requirement? Thank you, Jessica Jessica Piche, HT(ASCP) Waterbury Hospital Histology Laboratory

[Histonet] microtome blades and wax

Good Morning, We have been using Leica Histocore Autocut microtomes for a few months and have been having inconsistency with cutting on them. We have tried so many different blades, high and low, and have tried changing the blade angle. Nothing is a perfect fix, and we feel like we are really

[Histonet] slide printers

Good Morning, Happy Friday Everyone. I was wondering what people are using/recommending for slide printers at the microtome? Do they all have to be connected to an LIS? Thank you, Jessica Jessica Piche, HT(ASCP) Histology Team Leader Waterbury Hospital Histology Laboratory

[Histonet] Processing protocols

Good Morning Everyone, Our pathologist wants us to shorten our large tissue processing run so that we can run small biopsies with the bigger stuff when one of our tissue processors are down. We have 2 Excelsiors. Would anyone like to share any protocols they might have for this purpose? Thank

[Histonet] pan-TRK or NTRK antibody

Good Afternoon, Where are people buying the pan-TRK or NTRK antibody from? Pre-dilute preferred. Thank you in advance and have a great day! Jessica Piche, HT(ASCP) Waterbury Hosptial Waterbury, CT 06708 ___ Histonet mailing list

[Histonet] CAP Requirements for Scales and Balances

Good Afternoon All, Does anyone know if there are stated CAP requirements in regards to the calibration of scales and balances, and the intervals at which they should be calibrated? Thanks in advance and have a good day! Jessica Piche, HT (ASCP) Waterbury Hospital Waterbury, CT

[Histonet] bachelors of science

Hello Everyone, Do you need a bachelor's of science to sit for the HTL exam? I thought you had to have a bachelor's degree, and the right amount of credits in science, chemistry, etc. Thanks in advance. Jessica Piche, HT(ASCP) Waterbury Hospital ___

[Histonet] HSV 1&2 Control slides

Good Morning Everyone, I was wondering where everyone is getting control slides for HSV1&2 Antibody? I can't seem to find a lot of options. Thanks in advance! Jessica Piche, HT(ASCP) Waterbury Hospital ___ Histonet mailing list

[Histonet] Microtomes

Good Morning Everyone, We are in the market for new microtomes and was wondering what everyone is using out there? We are looking for semi-automated/automated microtomes. We are demoing the Leica Autocut and the Thermo 355S. I really like the Leica microtome but am not crazy about the control

[Histonet] grossing credentials?

Good Morning, One more question. I was wondering if anyone is having a problem being credentialed for grossing with an Associate in Science in Veterinary Technology? Our state DPH is questioning my degree as not being a laboratory technology. I know there are lots of techs working in

[Histonet] PAX-8

Good Morning, Is anyone using PAX-8 from Biocare on the Dako Omnis and if so would you mind sharing your protocol/control tissue? I'll also take any information on any Pax8 protocols from different vendors/instruments. Thank you, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY

[Histonet] Xylene resistant gloves and xylene replacers

Good Morning Histonetters: I have two questions. Are there any gloves in existence (that are user friendly) that are resistant to xylene? And are there any xylene replacers out there that are actually safer than xylene? Thanks in advance and have a great day! Jessica Piche, HT (ASCP)

[Histonet] CAP Gen 41770 glassware cleaning

Hi Everyone, I was wondering if anyone would mind sharing their procedure for glassware cleaning? We used to send our glassware down to Central Sterile supply but we have been bleaching our glassware/plastics and have had no issues with special stains. Is it a requirement to use detergent and

[Histonet] Harris Hematoxylin

Good Morning Everyone, Just wondering what kind of Harris Hematoxylin people are using. We are doing a regressive H on a Leica Autostainer XL for 9 minutes. We have been buying Ricca Hematoxylin but we are having so many issues with it. Are people using acidified Hematoxylin in regressive

[Histonet] Questions regarding microwave questions on Anatomic Pathology CAP Checklist

Happy New Year Everyone!! :) Questions on CAP Anatomic Path Checklist. Are the microwave questions referring to microwave processors/processing or the microwave oven that we use for special stains? Questions are ANP. 27170, 28290, 28860, and 29430. I'm thinking the microwave oven but I want

[Histonet] Her2neu SP3 clone

Hi Everyone! Happy Friday! I was wondering if anyone would mind providing the protocol for your SP3 IHC stain on the Dako instruments you have? We are setting up an Omnis and would like a starting point for the assay... Thanks, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email

[Histonet] Cryostat decontamination ANP23410

Hi Everyone, Just a quick question. If you defrost your cryostat to room temperature and wipe it out with 70% alcohol is that considered decontamination? Or does it have to be fumigated with formaldehyde, or have a UV function on board the machine? Thanks, Jessica Piche, HT(ASCP) Waterbury

[Histonet] FW: Freeze Spary Ban continued

Just checked ours: Richard Allan Scientific CYTOCOOL II has it as well!! Jessica Piche, HT(ASCP) Waterbury Hospital -Original Message- From: Macke, Gail via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Tuesday, March 01, 2016 1:13 PM To: Histology Cc:

[Histonet] Automatic coverslippers

Hi Everyone, Just looking for opinions hereWhat automatic cover slipper do techs like best? And why? Thanks, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information

Re: [Histonet] Decal of bone marrows

Hi Adrienne, It all depends on what you use for decal. We use 5% Nitric acid for 1 hour or so. Sometimes it needs a bit more time. Have a good weekend! Jessica -Original Message- From: Adrienne Anderson [mailto:rennie1...@yahoo.com] Sent: Friday, June 05, 2015 1:25 PM To:

[Histonet] All Common Question 30550

Hi Everyone, I was just wondering if everyone has a written procedure for performance verification for their tissue processors? And if so what your procedure entails? Performance verification reports from companies that fix the tissue processor or an actual program on the tissue processor to

Re: [Histonet] IHC billing question

Hi Joyce, What about if you are using 88360 for Estrogen receptor and Progesterone receptor? An example would be an order for ER, PR, p63, AE1/3? Would it be 88360 x 2, 88342, 88341 or would be it 88360 x 2 and 88341 x 2? I'm not sure if the quantitative charge takes precedence over the 88342

Re: [Histonet] Centrifuge for cytospins

Thanks for sharing Joe! :) Jessica -Original Message- From: Joe W. Walker, Jr. [mailto:joewal...@rrmc.org] Sent: Tuesday, April 28, 2015 2:31 PM To: Cooper, Brian; Piche, Jessica; histonet@lists.utsouthwestern.edu Subject: RE: Centrifuge for cytospins In all of my experiences

Re: [Histonet] Centrifuge for cytospins

Thanks for sharing Brian. Jessica -Original Message- From: Cooper, Brian [mailto:bcoo...@chla.usc.edu] Sent: Tuesday, April 28, 2015 12:10 PM To: Piche, Jessica; histonet@lists.utsouthwestern.edu Subject: RE: Centrifuge for cytospins We make our BAL cytospins directly--no centrifuging

[Histonet] Centrifuge for cytospins

Hi Everyone, Just asking a quick question for our Cyto department. Are BAL slides centrifuged and then made in to cytospin slides or do you just make the cytospin slides with no centrifuging? Thank you, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain

RE: [Histonet] histology in higher education

Thank you for sharing this information Andi. I'd like to do something like this and I'm going to send this on to my daughters science teachers at school. I think it's a great idea. It always amazed me all the different jobs in hospitals alone that are available for kids and adults alike and no

RE: [Histonet] Orange peel for GMS

I got a nice GMS control from some moldy chicken in my fridge. I am also trying the onion. I haven't stained the onion yet but it actually cut beautifully so I have high hopes. I let you know how it turns out. Have a nice weekend! Jessica Piche, HT(ASCP) -Original Message- From:

RE: [Histonet] Orange peel for GMS

Hahahaha I bet not Linda!!! Jessica -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Blazek, Linda Sent: Friday, April 17, 2015 12:05 PM To: Bitting, Angela K.; Piche, Jessica; 'T Williams'; histonet

RE: [Histonet] Orange peel for GMS

] On Behalf Of Piche, Jessica Sent: Friday, April 17, 2015 7:11 AM To: 'T Williams'; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Orange peel for GMS I got a nice GMS control from some moldy chicken in my fridge. I am also trying the onion. I haven't stained the onion yet but it actually cut

RE: [Histonet] p16

Thanks! From: Shannon Kleiner [mailto:shk...@bellin.org] Sent: Wednesday, March 04, 2015 4:12 PM To: histonet@lists.utsouthwestern.edu; Piche, Jessica Subject: Re: [Histonet] p16 Hello Jessica, We use Cintec p16 on our Bond Max. Here is a link you may find useful http://www.ventana.com

[Histonet] RE: Mushrooms for GMS fungus control

I will say I hacked a mushroom off of a tree once and processed it and it stained nicely for GMS. I also used some chicken that was in the fridge too long and we actually use that for our GMS control!! Jessica Piche, HT(ASCP) Waterbury Hospital -Original Message- From:

[Histonet] p16

Hi Everyone, I was wondering if people are using the p16 antibody and if so where are you getting it from? Thank you, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This

[Histonet] MERIFLUOR DFA Pneumocystis

Good Morning, I was wondering if anyone uses the Merifluor DFA kit for Pneumocystis and if so where can you buy controls for it? We have had no positive cases to make our own cytospin controls. Thank you, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any

[Histonet] Balance and Scale Calibration

Hi Everyone, I was wondering what other anatomic pathology labs are doing to calibrate their scales and balances? I also would like to know what the basic tolerances for balances or scales is? I can only seem to find information in laboratories that deal with soil. I have a procedure and a

[Histonet] CAP Question CYP.04300 Daily QC

Hi Everyone, I am asking a question for our Cytology department about CYP.04300 on the CAP checklist, in regards to daily QC. It states the technical quality of the preparations should be checked daily and includes checking all stains for predicted staining characteristics each day of use.

[Histonet] Scale or Balance

Hey Everyone, Does anyone know of a good brand/vendor for a balance (with a weighing dish) for the gross room? Thanks, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is intended

[Histonet] COM 30450

Hello Everyone, Does COM 30450 apply to Diff Quik kits and reagents used in special stains (other stain kits)? Or does the lot to lot testing only apply to things such as IHC? Thank you, Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential

[Histonet] Xylene testing ANP 08216

Hey Everyone, Just curious what people are doing with xylene testing? Are people really only doing the initial testing and never testing again if all was good? Seems like there should be periodic testing after decades have gone by! Thanks, Jessica Piche, HT(ASCP) Waterbury Hospital

[Histonet] ANP08216 Formaldehyde and xylene testing

Thanks for all the responses so quickly. I'd also like to know the frequency of formaldehyde testing as well? Thanks to those who included that as well. Jessica Piche, HT(ASCP) CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged.

[Histonet] Frozen section PPE

Hi Everyone, We were wondering what people are wearing while doing frozen sections? Mask, goggles, lab coat? We'd like to know what everyone else is doing to protect themselves. Thank you, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments

[Histonet] Retic stain kits

Hello Everyone, Can anyone recommend a good retic staining kit that is reasonably priced and works well? Thanks, Jessica Piche, HT(ASCP) Waterbury Hospital CONFIDENTIALITY NOTICE: This email and any attachments contain confidential information that is legally privileged. This information is

RE: [Histonet] CAP Survey Question

More viewing with the pathologist would be great! Jessica Piche, HT(ASCP) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of joelle weaver Sent: Friday, December 06, 2013 8:07 AM To: Amos Brooks;