Fellow histonetters,
I'm looking for advice for cutting bone samples embedded in LR white resin on a
microtome.
We have plenty of experience cutting decalcified bone in wax and with
sawing/grinding sections, but little experience in cutting thin sections of
resin embedded bone.
We will be
I work with bone samples trimmed to approx 35mmx35mmx4mm. Our general procedure
is:
Fixation:
untrimmed samples stored in 10% NBF @4°C until trimming. Slices placed in fresh
10% NBF for at least 12h.
Decalcification
Benchtop - 10% formic acid for 10 days (change soln every 3 days), rinse for
Hi all,
A couple of weeks ago I posted a message about needing a new supplier
for Sanderson's RBS now that Surgipath/Leica have stopped selling it.
I've just had confirmation that Dorn Hart Microedge, Inc in Chicago
will be taking over the manufacture and supply of Sanderson's RBS. Stain
I'm looking for a new supplier for Sanderson's Rapid Bone Stain as Surgipath
have stopped selling it (at least in Europe).
Does anyone know of an alternative supplier or an equivalent stain I could
substitute in?
[Currently use the RBS on 15µm ground Technovit 7200VLC resin sections, with
acid
