[Histonet] cross reactivity of CMV and HSV1&2 antibodies

Hi everyone, Has anyone encountered the cross reactivity of CMV and HSV1&2 in human placental tissue? We had a patient case that stained positive for these antibodies in the same tissue section. My antibody specification sheets state that there is no cross reactivity, but I have found

[Histonet] p40 staining for breast myoepithelial cells

Hi everyone, Are any labs using the monoclonal p40 antibody for myoepithelial cell staining in breast tissue? The pathologist that I work with has me testing this antibody to be used to differentiate between lung squamous and adenocarcinoma, but is also wondering if labs are using it on breast

[Histonet] p40

Hi everyone, I'm looking into ordering the p40 antibody for one of our pathologists. He wants to use it to help distinguish between adenocarcinoma and squamous cell ca in lung. I'm wondering what labs are using, the monoclonal or polyclonal antibody, and where you purchase it from. I haven't

[Histonet] p57

Hi everyone, Would anyone be willing to share information about what clone/company/dilution you use for p57? We are looking to use this antibody on formalin fixed/paraffin embedded human placenta. Thanks, Jean Taylor, HT(ASCP)QIHC Immuno Tech Meriter Health Services Madison, WI

[Histonet] CK AE1/AE3

Hi Everyone, I'd like some input as to what labs are using as a procedure for this antibody. Is enzyme digestion or a form of antigen retrieval with heat used? What dilution and detection system do you use as well as what type of machine is it run on? Thanks for the info, Jean Taylor, HT

[Histonet] AMACR (P504S)

Hi Everyone, Our pathologists are interested in using this antibody to detect metastatic renal cell carcinoma in paraffin sections. They are not interested in an antibody cocktail, but also want to use it to detect prostate cancer. I'd like to know what company and clone most labs are using.

[Histonet] batching controls

Hi Everyone, I'd like to know how many labs batch their controls when the same antibody is ordered on multiple cases. Do you run the control on a separate slide, or with one of the cases ordered? Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI

RE: [Histonet] batching controls

Miller [mailto:cmil...@physlab.com] Sent: Tuesday, January 11, 2011 12:16 PM To: Galbraith, Joe; Rathborne, Toni; Laurie Colbert; Taylor, Jean; ih...@googlegroups.com; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] batching controls We run 1 control per antibody within a case, so if we

[Histonet] Eosinophil peroxidase antibody

Hi Everyone, Is anyone using this antibody? If so, could you please provide the source of where you purchase it? We would be using it on paraffin sections with Dako instrumentation. Thank you, Jean Taylor, HT(ASCP)QIHC IHC Tech. Meriter Health Services Madison, WI

[Histonet] MPB antibody

Hi Everyone, Has anyone worked with the MBP (human eosinophilic major basic protein) antibody? If so, could you please provide the source of where you purchase it? We would be using it on paraffin sections with Dako instrumentation. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health

[Histonet] PAX-5 - B-cell specific activator protein (BSAP)

Hi Everyone, I'm trying to find out what company and clone most labs are using for PAX-5 (BSAP). Thanks! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI -Original Message- From: histonet-boun...@lists.utsouthwestern.edu

[IHCRG] RE: [Histonet] PAX-5 - B-cell specific activator protein (BSAP)

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[Histonet] ER clone 1D5 or SP1 ?

I'm wondering which clone of ER most labs are using? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] DAKO Autostainer Link 48/FLEX system

Hi Everyone, I would like to hear (pros and cons) from labs that have this instrument or have looked at it. We currently use 2 of the oldest Dako autostainers and are in need of updating. Any feedback would be appreciated. Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services

[Histonet] CISH - hybridizer

If you do not have heat on your IHC stainer, what instrument do you use for denaturing and hybridization? Does anyone have experience using the MJ Research PTC -100 with in situ slide block from Pegasus Scentific? Thanks, Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI

[Histonet] CD15 clone

Hi everyone, I'd like to hear what clone and company labs are using for their CD15 antibody. Thanks! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] CISH - HER2

The pathologists that I work for are requesting that our lab look into starting up CISH mainly for HER2. What company are labs purchasing their CISH HER2 kits from? Does anyone use Dako's DuoCISH kit along with their HER2 FISH pharmDx kit? We currently run the old Dako Autostainers, but are in

[Histonet] CMV antibody

Hi Everyone, I'm wondering what antibody clone labs are using to detect Cytomegalovirus by IHC. Thank you! Jean Taylor, HT(ASCP) QIHC IHC Tech. Meriter Health Services Madison, WI 53715 ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] CMV

Hi Everyone, The pathologist that I work with wanted me to add that the CMV that we're staining for is in the GI tract, and he's wondering if there is a different clone/procedure that labs use for this? Thanks again!! Jean Taylor, HT(ASCP) QIHC IHC Tech Meriter Health Services Madison, WI

[Histonet] MMR protein antibodies for Lynch Syndrome

Hi Everyone, I'm looking for information about MLH1, MSH2, MSH6 and PMS2 and any other IVD antibody that labs are using to identify Lynch Syndrome. Would appreciate any information regarding antibody source, retrieval methods, dilutions, etc... Thank you! Jean Taylor, HT(ASCP) QIHC IHC Tech

[Histonet] TdT

I'm wondering where labs are purchasing their TdT antibody from. I'm currently using the Dako Autostainer and would be interested in any information on the procedure as well. Thank you! Jean Taylor, HT(ASCP)QIHC IHC Tech Meriter Health Services Madison, WI

[Histonet] IHC and ISH

Hi everyone, I am currently in the process of evaluating IHC equipment to replace my old Dako Autostainers. The pathologists I work for are interested in bringing ISH into our lab. My questions are, what IHC instrument have you found works the best for IHC and ISH, and what kind of training did