Hi Everyone,

I'm trying to find out what company and clone most labs are using for PAX-5 
(BSAP).

Thanks!

Jean Taylor, HT(ASCP)QIHC
IHC Tech
Meriter Health Services
Madison, WI

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Subject: Histonet Digest, Vol 81, Issue 23

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Today's Topics:

   1. RE: CD34 positive control (McMahon, Loralee A)
   2. Ventana Renaissance Containers (ricky hachy)
   3. CD34 Control (Silverman, Jeffrey)


----------------------------------------------------------------------

Message: 1
Date: Wed, 18 Aug 2010 12:17:39 -0400
From: "McMahon, Loralee A" <loralee_mcma...@urmc.rochester.edu>
Subject: RE: [Histonet] CD34 positive control
To: Joel Reichensperger <jreichensper...@siumed.edu>, Histonet
        <histonet@lists.utsouthwestern.edu>
Message-ID:
        
<c27aa2a01cef31469813089e226f582e02d5a7c...@urmcms7.urmc-sh.rochester.edu>

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Just about any tissue should work.   It stains for Hematopoietic 
stem/progenitor cells, bone marrow stromal cells, endothelial cells, embryonic 
fibroblasts
We use a sausage style control here with about 30 different types of tissue.


Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-boun...@lists.utsouthwestern.edu 
[histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Joel Reichensperger 
[jreichensper...@siumed.edu]
Sent: Wednesday, August 18, 2010 12:05 PM
To: Histonet
Subject: [Histonet] CD34 positive control

  I have a doctor who wants to stain some tissue with cd34. I need to
know if anyone can recommend a good positive control tissue for this
antibody. The staining will be done in paraffin embedded sections.
Thanks in advance.

Joel

--
Joel Reichensperger
Researcher II
Southern Illinois University
Plastic Surgery Institute
jreichensper...@siumed.edu
217-545-7309 (Office)
217-545-1824 (Fax)


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------------------------------

Message: 2
Date: Wed, 18 Aug 2010 16:28:39 +0000
From: ricky hachy <elc...@hotmail.com>
Subject: [Histonet] Ventana Renaissance Containers
To: Histonet <histonet@lists.utsouthwestern.edu>
Message-ID: <snt114-w54ab5f08e7e0cfc97193edcf...@phx.gbl>
Content-Type: text/plain; charset="iso-8859-1"


Hello ,

I need 4 good plastic containers, where you fill with xilene,alcool....... for 
the VENTANA RENAISSANCE .

Anyone could sell me ?

Regards

Ricky

------------------------------

Message: 3
Date: Wed, 18 Aug 2010 12:30:58 -0400
From: "Silverman, Jeffrey" <jsilver...@nshs.edu>
Subject: [Histonet] CD34 Control
To: "'jreichensper...@siumed.edu'" <jreichensper...@siumed.edu>
Cc: "'histonet@lists.utsouthwestern.edu'"
        <histonet@lists.utsouthwestern.edu>
Message-ID:
        <83f4d81747a7094dafe3ae87151ecb941ce4144...@sykechxvs01.nslijhs.net>
Content-Type: text/plain; charset="us-ascii"

Joel,
A section of skin will be a fine CD34 control. All collagenous connective 
tissue, including the dermis,  are rich in CD34+ dendritic interstitial 
fibroblasts as well as CD34 positive endothelium in the resident vessels. 
Actually, in my lab we use a section of fallopian tube from tubal ligation, 
they are loaded with the fibroblasts and vessels.

Jeff Silverman



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