Many thanks to all who contribute and keep the histonet running. I'm working my
last day here at USDA-ARS ADRU within WSU which has been a very rewarding and
enjoyable occupation. I was honored to be on 14 publications in 12 years with a
few more in the works. Due to Federal budget constraints,
When I solve your equation V1 X 1560 ug/ml = 10ml X 0.8 ug/ml I get .005 ml.
Therefore the dilution would be closer to 1:2000. I would usually divide the
1560 ug/ml by 0.8 ug/ml and get 1950 therefore 1:1950 would be more accurate .
Tom T
-Original Message-
From:
You are so right-that's why I always try to double check everything even my
spellink. Tom T
-Original Message-
From: Elizabeth Chlipala [mailto:l...@premierlab.com]
Sent: Friday, November 21, 2014 12:05 PM
To: Truscott, Tom; 'histonet@lists.utsouthwestern.edu'
(histonet
Hi Molly, You might just want to fix in buffered 10% formalin and then rinse
specimens well in water ( maybe 20 minutes) before using in class and then put
back in formalin. Tom T
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Hi Adelle, Biopsy sizes will matter, but you might shorten some of these times,
but be sure to add at least one more Histoclear to make sure there is no
alcohol in it and at least one more paraffin to make sure there is no
histoclear carryover.If you go back to 70% at any time you will have to
Hi Roberta, Check with those requesting to see if you can cut the equilivant
but with thicker sections that will roll- like 5 20's or 2 50's. The rolls are
lots easier to get into the tubes. Tom T
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Hi Sharon, If you are trying to be secure enough against tampering that it
would stand up in court- the zip tie method probably isn't enough. I would try
to get a used small set of the larger locking safe deposit boxes or maybe a
group security box from grainger. Tom T
-Original
Hi Cassie, For me, it has been mostly positive changes. I went from feeling
like a robot cranking out slides and rarely hearing about any impact on the
patient to being involved from start to finish on published articles in
research journals. I've been able to use cutting edge technology to
Hi Judith, If you have made completely fresh solutions, even any stock reagents
and still have the problem, then the fungus may be arriving from another
source. I once had a problem of pollen landing on my water bath and showing up
on the stains that would detect it. You may have fungus on
Hi Cheryl, If you can, use more eggs. Then process them into a pellet with
histogel. Upon sectioning, many different cross-sections will be visible in the
same section. Tom T
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
Hi Abby, Since the concentration is about a third of the original, I would try
programming the XT to run the primary for 2 hrs instead of 32 min. You may also
need to up the secondary time if that isn't sufficient. Tom T
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
We have both upright and chest and both have their pros and cons. We have had
trouble with electrical circuits which aren't matched to the load that these
freezers can put on them. An electrician told us that it can put an tremendous
strain on the freezer motor if the circuit isn't right and
Hi Jackie, I haven't done a lot of eyes, but mainly sheep lenses. I seemed to
have more trouble with crushing artifact when trimming in- so I tried not to
trim too close and do the closer trimming on the microtome- I also trimmed
before they got too hard from fixing. Tom T
-Original
Hi Cherie, Since the top section is consistently better, then the possibilities
lie in what happens after they are on the slide. As Toni recommends slides that
are heated or deparaffinized vertically may have left over paraffin on the
lower regions.Maybe you could also try depar with them up on
If anyone has had success with solving background issues with Ventana's Q-Dot
labeling, would you please contact me? Thankyou, Tom Truscott
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Hi Kathleen, Several years and a couple employers ago we attached small
biopsies (for proper orientation) to small squares of cucumber (which had been
dehydrated in several changes of alcohol) with a egg-albumin/glycerol mixture.
The bx/cucumber unit was embedded together after processing and
I haven't used HP, but have noticed this stippled effect especially when using
40X on the microscope and looking at slides labeled with an Ventana alk-phos
kit. I seem to have solved the problem by making sure no trace of H2O is left
on the slides. It helps after washing slides to run them thru
If the xylene was not recycled properly and retained too much alcohol, then the
extra alcohol could be the culprit and do the drying'. Tom T
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Rene J Buesa
Hi Wayne, I had lots of problems with round irregular crystals, but have
greatly improved my slides by limiting baking at 57 degrees to 1/2 hour. We use
a paraffin with plastic in the mix, and I think the plastic globs up under some
conditions, like no or not enough xylene to dissolve it out.
To those with the Biocare intelliPATH system, Is the antigen retrieval part of
the IHC automated or do you need to use the Biocare decloaker? Thankyou, Tom
Truscott
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Hi Jennifer, I am by no means an expert on this, but have done a few mouse
guts. I think that trying to flush out the feces with formalin or saline soon
after necropsy, would help preserve the mucosa. The bacteria in the gut start
breaking down the mucosa soon after death. Perhaps the gut is
Hi Sally, I think I would ask if they were familiar with the terms bovine,
porcine, ovine, caprine, equine, ect. Then ask if they would process bovine
tissue differently than mouse tissues. Ask what kind of problems they might
expect to encounter doing IHC and immunofluorescence on animal
Hi Beth, Saffron is a great spice. We use it in a old family recipe from
Cornwall for a bread roll( saffron nubbies). We also find the price very
expensive, but also varies a lot. If you know anyone in the middle East, or
India, they could get it a lot cheaper. We have raised it in our garden
Or, has you tried gently infusing the intestinal lumen with formalin from a
syringe and tying or clamping both ends, then allow to fix in a jar of
formalin, then trim open lengthways, then trim C-shaped cross-sections, then
remove fecal material before placing in cassette. Tom T
-Original
Hi Susan, I would suggest cutting lots thinner sections. The plus slides seem
to have trouble holding on to very thick sections. Another possibility is too
much glacial acetic acid in your working solution of cresyl-too much acid can
loosen the bonds on the plus slides. Tom T
-Original
Hi All, Is there a comprehensive list in one location somewhere that gives
information on all or most antibodies on how they work with FFPE or FS or other
fixatives, so that we don't have to try to many approaches when we get a new
antibody to try. Thanks, Tom T
...@lists.utsouthwestern.edu] On Behalf Of Truscott, Tom
Sent: Friday, March 18, 2011 9:02 AM
To: Histonet
Subject: [Histonet] comprehensive list?
Hi All, Is there a comprehensive list in one location somewhere that gives
information on all or most antibodies on how they work with FFPE or FS or other
That sounds like the one TBS made or makes. Tom T
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of louise renton
Sent: Wednesday, January 05, 2011 10:39 PM
To: Cameron Nowell; Histonet
Subject: Re:
I am seeking basic information on PLP fixative. What is it? Can I make it or
buy it? How long does fixation take for lymph node? Thanks in advance, Tom
Truscott
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One way to think about it, would be that if a pathologist accepts a case, it is
understood that he or she has the expertise to diagnose it, and with a
diagnoses comes a bill. But, if unable to diagnose, or confidently diagnose,
and a consult is needed, then the pathologist that can make the
Hi, The old TBS's are blue, and the new ones are white. Tom T
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Cynthia Pyse
Sent: Tuesday, June 08, 2010 12:02 PM
To: 'Cheryl';
If not already mentioned, wiping your heated forcep off with a kimwipe or paper
towel before and after embedding each tissue helps prevent this problem at
embedding.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On
Hi Wanda, Soaking tissues in white vinegar tends to clear the fat and make
the lymph nodes more visible, but it might affect some tests. Tom Truscott
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Hi Abijag, You can get MSDS forms from the company that explains any hazards
associated with these reagents or any others. You can study these with your
local distributor. These reagents are packed by RA to be safely shipped
anywhere in this country and I would assume wouldn't need much extra
Hi Stella, Not only wiping the top of the waterbath water with kimwipes between
each block, and keeping forceps clean at embedding, and keeping your slides
clean, but also keeping things clean at grossing: clean cutting board and
instruments between tissues or cases. One pathologist called it
Hi Ann, Are you using the same microscope in the new location? Tom Truscott
USDA-ARS
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
thisis...@aol.com
Sent: Friday, February 06, 2009 1:09 PM
To:
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