[Histonet] Please remove my name

Hi, please remove my name from the mailing list. Thank you ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] FISH

Hi everyone, I was wondering if anyone would like to share their protocol for FISH on PFA fixed tissues? Any help would be hugely appreciated. Thanks so much. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] stains for visualizing new bone growth

You could do a modified tetrachrome stain, this distinguishes newly woven bone. On 2/1/11 2:44 PM, Robin Dean robin_d...@compbio.com wrote: Does anyone know of a good stain to use to clearly show new bone growth other than von Kossa stain? Would appreciate any suggestions anyone might have.

Re: [Histonet] positive slides

We use VWR Superfrost Slides Cat # 48311-703 and they are great, no lifting sections. On 12/1/10 1:57 PM, zodia...@comcast.net zodia...@comcast.net wrote: Hello, Can anyone recommend a brand of positive slides that are good for IHC. We are having problems with tissue falling off of the

[Histonet] Biocare¹s Rodent Decloaking Solution

Hi everyone, For anyone that uses Biocare's Rodent Decloaking Solution, once the solution has been made up, can it be reused? And if so in your experience how many times? Many thanks in advance ___ Histonet mailing list

[Histonet] Bone IHC

Hi everyone, I was wondering if anyone has any tricks on how to get bone sections to stop lifting off the slide through the IHC process? I leave them in the oven for quite a while to make sure they are baked on, however after antigen retrieval (pressure cooker for 20mins) most of the boney

Re: [Histonet] Osteoclast

You can do TRAP ( Tartrate-resistant Acidic Phosphatase) stain for osteoclasts. On 10/5/10 4:58 PM, Lin Bustamante lbustama...@cvm.tamu.edu wrote: We need to find a way to stain mainly Osteoclast. Any suggestions? Thank you very much. Lin Lin S. Bustamante, B.Sc.; HT(ASCP) Research Associate

[Histonet] Laboratory move

Hi everyone, I was wondering if anyone may have a laboratory relocation guidelines/checklist? Thanks so much ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] TUNEL

Hi everyone, I am hoping you can help me I am looking for some advice on how to do TUNEL staining (fluorescent or enzymatic), kits recommended/ protocol. Any help would be greatly appreciated. Thanking you in advance ___ Histonet mailing list

[Histonet] Commercial Eosin

Hi everyone, Has anyone had the experience of massive overstaining with commercial Surgipath Alcohol-based Eosin? I manually stained with Surgipath Hx for 2mins, then bluer the eosin for 30secs and the whole tissue was completely pink! Thanks V ___

[Histonet] IF

Hi everyone, When carrying out IF on frozen sections how long after the cutting the sections should you leave the slides to dry and where before starting the staining procedure? Thanks a mill, V ___ Histonet mailing list

[Histonet] IF on frozen sections

Hi everyone, Does anyone have any experience in IF on mouse embryo frozen sections? Vanessa ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] Section thickness

Hi Guys, Just wondering what thickness you cut sections at? I was always used to cutting at 2-3 microns in my last lab, however in my new place they are cutting at 6 microns (for both H Es and IHC), which seems to me as really quite thick! What would be the average cutting thickness? Thanks a

Re: [Histonet] Section thickness

that they would mind if you cut thinner, but they will mind if you start bringing this issue about. Let your thinner sections speak for themselves. It will get the moment that by example your way will prevail. René J. --- On Tue, 3/3/09, Vanessa J. Phelan vjp2...@columbia.edu wrote: From

[Histonet] Ideal fixation of mouse prostate

Hi everyone, I am very new to histonet, actually just joined.I hope you guys can help. I was wondering if anyone has any experience in the fixing and processing of mouse issues, namely prostate or bladder. I am currently trying to optomize protocols for this as I have very recently taken on the