Re: [Histonet] Advice for new grads?

2022-05-09 Thread Victoria Baker via Histonet
Pam, So far a lot of excellent advice has been given. Along the lines of what Tim has said, learn new tests. Any chance to get scope time with a pathologist one on one or in a multi scope setting is an excellent learning tool. It shows your interest & you will get to know your pathologists. This

Re: [Histonet] autopsy blocks

2021-06-04 Thread Victoria Baker via Histonet
Hi! We are required to keep all of our materials for 20 years. CAP I think is less, but we have to follow NYS. Vikki Baker On Fri, Jun 4, 2021 at 1:22 PM Nancy Schmitt via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello- > > Can you tell me what you are doing with autopsy blocks?

Re: [Histonet] Question - Bone marrow core biopy specimens

2021-05-03 Thread Victoria Baker via Histonet
Hi Richard! That is the duplicate of what we do in our lab. We were putting both the core and the clot on the same slide for in house testing, but den outs we sometimes have to split the specimen and only on a slide. I have a question for you about ISH Kappa/Lambda. What decal protocol do you

[Histonet] Frozen section QA

2020-10-26 Thread Victoria Baker via Histonet
Happy Monday morning! We recently had an incident which led us to review our policy on frozen section QA. In your institution does the same pathologist who did the frozen section read the permanent sections? Also if they do then who is the pathologist responsible for doing the QA? How is this

Re: [Histonet] QIHC Prep

2020-09-14 Thread Victoria Baker via Histonet
Ariel, Go to the NSH learning they have a very good tool there. Vikki On Mon, Sep 14, 2020, 10:29 AM Ariel Liberda via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hello all! > I hope everyone is staying safe! > > I'm looking to get my QIHC and I was wondering what prep materials >

Re: [Histonet] question about Digital Pathology Certificate

2020-04-08 Thread Victoria Baker via Histonet
Ranna, I took the course and it was well worth the cost for me. As to how it is assisting my career right now it isn't as my facility stopped using imaging about a year and a half ago. With the pandemic they may be rethinking this, but it won't be immediate. This type of skill will be more

Re: [Histonet] CPT Code 88172

2019-09-04 Thread Victoria Baker via Histonet
Hi, I just referred back to my sometimes frustrating clear as mud 2019 CPT coding binder. 88172 is not saying that it has to be done by a pathologist. In many cases it is completed by a Cytotechnologist. The precise wording says "Cytopathology, evaluation of fine needle aspirate; immediate

[Histonet] Roche Symphony and Ultra users

2019-08-30 Thread Victoria Baker via Histonet
Happy Friday! I'm looking to see if anyone out there is using these two specific instruments together. For about 8 or 9 months we have been experiencing what looks like water or water droplets on the IHC slides. It's not on all slides and we can go a span of time with no issues only to have it

Re: [Histonet] CD27

2019-08-06 Thread Victoria Baker via Histonet
On Mon, Jul 29, 2019, 9:45 PM Victoria Baker wrote: > Hi! > > Is anyone currently using CD27 on FFPE human tissue? > > I found one source and it is for lyophilized CD27. I'd like to find one > that is a concentrate. I have given up on a pre-dilute. I would like to > see if there is both a

[Histonet] CD27

2019-07-29 Thread Victoria Baker via Histonet
Hi! Is anyone currently using CD27 on FFPE human tissue? I found one source and it is for lyophilized CD27. I'd like to find one that is a concentrate. I have given up on a pre-dilute. I would like to see if there is both a mono and a poly clonal ab available and is I'm use. Thank you in

Re: [Histonet] Use of Freeze Spray in a Cryostat

2019-05-28 Thread Victoria Baker via Histonet
Jennifer, I called Leica as we have 2 cryostats at different locations so we needed to be sure that we could use our current spray that we get from Leica. We were told that their spray with the 1,1,1,2 Tetrafluoroethane is safe to use. I would therefore check to see what you have in stock and if

[Histonet] ISH kappa lambda on bone marrow core biopsy

2019-03-27 Thread Victoria Baker via Histonet
Good morning! Just recently we started having an issue with a heavy blue precipitate on our bone marrow cores. We have changed nothing in our protocol and even did parallel testing with a reference lab. Both had the precipitate, but to a lesser intensity. Has anyone else been seeing this?

Re: [Histonet] Air pocket in paraffin blocks

2019-03-08 Thread Victoria Baker via Histonet
Thank you everyone for your responses. It is very much appreciated. It is an up hill issue as most of the technical staff have been here and maybe one other lab in their entire career and "no one's complained before". I'd like that phrase and "that's the way we've always done it" stricken from

Re: [Histonet] Roche "bashing"

2019-03-06 Thread Victoria Baker via Histonet
Kelly, It is not bashing, it is frustration and concern. We need our instruments and reagents to work so that we deliver reliable and consistent results to our pathologists. They trust our work and dedication to them and ultimately the patient. When something like this happens and we can't get

Re: [Histonet] Ventana dispensers - last word

2019-03-05 Thread Victoria Baker via Histonet
Terri, We went through hell with the Roche dispensers in late 2017 to early 2018. The only thing positive I have to say is that the field engineer and our FAS are great. I have little respect for the sales staff or inside support. We pay top dollar for these instruments and the required

Re: [Histonet] Air pocket in paraffin blocks

2019-02-22 Thread Victoria Baker via Histonet
> tissue? > > I only re-embed blocks with air bubbles if it will affect the quality of > the slide myself. > > > Jennifer > > -Original Message- > From: Victoria Baker via Histonet > Sent: Thursday, February 21, 2019 4:49 PM > To: histonet@lists.utsout

[Histonet] Air pocket in paraffin blocks

2019-02-21 Thread Victoria Baker via Histonet
Hi, When I was trained to do embedding there were many things that the professor stressed to me need to be done in order to have the tissue block acceptable for sectioning. One of these was air bubbles. Recently we had a new tech embed a derm block that had an bubble that was pretty big. The

Re: [Histonet] Closed doors in Histology

2018-11-16 Thread Victoria Baker via Histonet
Mike, Yes that is correct. Histology labs because of their toxic chemicals and less than pleasant odors need to keep the doors shut for proper ventilation. This doesn't always happen when there is a high volume of people going in and out. I like it to the requirement that there be a sink (clean

[Histonet] HIF-1alpha and EBNA1

2018-09-04 Thread Victoria Baker via Histonet
To All, I am seaching for a lab that can run both of these antibodies on human FFPE tissue. HIF-1 alpha is Hypoxia Inducible 1 alpha EBNA1 is Epstein Barr Nuclear Antigen 1 If anyone performs these tests or knows of a lab that does, please contact me. Thank you in advance Vikki

Re: [Histonet] HT Position in Alaska

2018-06-06 Thread Victoria Baker via Histonet
Me as well. It's beautiful there! On Wed, Jun 6, 2018, 12:27 PM Colleen Forster via Histonet < histonet@lists.utsouthwestern.edu> wrote: > This sounds wonderful..I wish I were in a position to swoop it up! > > Colleen Forster HT(ASCP)QIHC > > On Wed, Jun 6, 2018 at 9:24 AM, Arrington, Karla

[Histonet] Epic and Cerner users, looking for feedback on these systems

2017-11-17 Thread Victoria Baker via Histonet
Happy Friday! Our hospital is starting to look at new HIS vendors. The two that are being strongly looked at are Cerner and Epic. Of course the hospital is using a consulting firm who have sort of tied our hands in terms of following up with vendors and any after the demo questions we are to

Re: [Histonet] Ann Preece Manual

2017-10-28 Thread Victoria Baker via Histonet
We currently have a student in our lab. She took her classes through Broome community college were the program is in its infancy. I think she would benefit from this book. I can give you contact information off line and it would be a hospital address. Vikki Baker UHS Wilson Hospital On Oct

Re: [Histonet] Ann Preece Manual

2017-10-28 Thread Victoria Baker via Histonet
We currently have a student in our lab. She took her classes through Broome community college were the program is in its infancy. I think she would benefit from this book. I can give you contact information off line and it would be a hospital address. Vikki Baker UHS Wilson Hospital On Oct

Re: [Histonet] IHC counterstain offline

2017-10-12 Thread Victoria Baker via Histonet
Curt, I am not sure how much you will actually save if you factor in the cost of a person hand counter staining these slides. I will be the first to say Ventana is over priced, but off line counter staining brings in an additional variable and cost. In the end is it a real savings? It's an

Re: [Histonet] on call autopsy services

2017-08-17 Thread Victoria Baker via Histonet
Have you looked at an ad locum pathologist? Or a retired pathologist that would be willing to work as a per diem? The county coroner might even be willing to get you information or know of a pathologist willing to come in. But do you have a dinner or a Histologist willing to do the assisting?

[Histonet] For NYS laboratories

2017-07-11 Thread Victoria Baker via Histonet
I'm trying to reach out to other NYS Histology labs. I can't really get a real answer regarding a non-CLT doing the embedding/microtomy and staining of just Autopsy material. I think that they can't but the actual documentation is not completely clear. What are other labs doing? Thank you in

Re: [Histonet] Stand Alone Heat Extractor

2017-05-15 Thread Victoria Baker via Histonet
ThermoFisher. It is on there website. I don't have the catalog number but I just ordered one On May 15, 2017 12:48 PM, "Amanda Reichard via Histonet" < histonet@lists.utsouthwestern.edu> wrote: > Hi Everyone > > I am hoping someone can help me out here... I am in search of stand-alone > heat

Re: [Histonet] Great input on recycled reagents

2017-02-22 Thread Victoria Baker via Histonet
By using a hygrometer (did I spell that right?) you should be able to see the purity of the alcohol. We usually get between 97 to 98 percent. We do use fresh absolute alcohol always in the processor. We start with 80, 95 all recycled. On Feb 22, 2017 4:55 PM, "Gareth Davis via Histonet" <

Re: [Histonet] Recycled reagents in VIP processor

2017-02-22 Thread Victoria Baker via Histonet
We routinely recycle xylene and alcohol on a CBG recycler without issues. We also run ER PR HER2 breast panel, ISH and routine IHC Vikki Baker On Feb 22, 2017 3:37 PM, "Gareth Davis via Histonet" < histonet@lists.utsouthwestern.edu> wrote: > Hi, > I was always told not to use recycled

[Histonet] Dialyzed iron solution

2016-06-24 Thread Victoria Baker via Histonet
Hi. Back when I was in research we did Hale's Colloidal Iron stain. Making the dialyzed iron was really the big part and back then we were using a cellulose tubing that we had from another group. I've been trying to find the right type but there is just too many choices. I've also been trying

[Histonet] Cell Blocks

2016-04-08 Thread Victoria Baker via Histonet
Hi - Happy Friday! Is anyone on makeing cellblocks with thromboplasting/plasma on cells fixed in sodium citrate? These would be pleural fluids and bronch brushings and washings. Thanks in advance. Vikki ___ Histonet mailing list

[Histonet] Thromboplastin for cell blocks

2016-02-24 Thread Victoria Baker via Histonet
Hi I am looking for Thromboplastin to make cell blocks. I can't remember who we got it from years ago. Any information is much appreciated. Vikki ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] IHC Weekend Coverage

2015-08-23 Thread Victoria Baker via Histonet
We have Saturday lab coverage which includes IHC. Case/slides vary between 8-15/25-85. This includes multiplex and ISH. Vikki On Aug 23, 2015 10:49 AM, Cartun, Richard via Histonet histonet@lists.utsouthwestern.edu wrote: How many of you working in hospital-based pathology laboratories run

Re: [Histonet] Breast fixation

2015-08-03 Thread Victoria Baker via Histonet
Karen We take ours off on Sunday. The tissue processor is set up with 6 hours of fixation the minimum time. We have VIP 5's so on Friday the tissue goes on a delay run that is set to come out of formalin at 12:30 AM Sunday morning. The end of the process is at 9 AM Sunday morning. Just take