[Histonet] Job Opening

2009-11-23 Thread Walzer Susan
We have an opening for a tech, days no weekends at St Pete.General Hospital in St Pete,FL. Call our HR dept at 727 384-1414 X 4814. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: Re:[Histonet] formalin storage

2009-09-14 Thread Walzer Susan
I think HCl and formalin when combined form a carcinogen, at least that is what I was taught many years ago. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of ti fei Sent: Friday, September 11, 2009 10:23 AM

[Histonet] RE: Ink issues

2009-09-02 Thread Walzer Susan
My Docs use acetone. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Amspacher, September Sent: Wednesday, September 02, 2009 7:03 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Ink issues G

[Histonet] RE: Gram Control

2009-06-15 Thread Walzer Susan
We take some fresh tissue (we use umbilical cord) and have micro incubate half with gram positive and the other with negative. After a few day we fix the tissues and put a piece of each in blocks. We then do a modified gram stain using acetone and the stain always looks great. See below: III.

RE: Re: [Histonet] Tissue processors

2009-03-13 Thread Walzer Susan
I have not heard much good about the Shandon processor. For reliability go with a VIP every time. This comes from MANY years in the field. I have never had problems with one. -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern

[Histonet] RE: We are buying a new Tissue Processor and need imput

2009-03-05 Thread Walzer Susan
After 30 years in histology I say go with VIP for reliable processing. (never had one fail or damage tissue) -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Farnham, Lori Sent: Wednesday, March 04, 2009 9:

[Histonet] RE: bone saws?

2008-11-26 Thread Walzer Susan
We use a Fleetwood (used by meat cutters) then have maintenance attach a plexi-glass shield. -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Jacqueline Farnsworth Sent: Monday, November 24, 2008 3:30 PM To: Histonet Subject: [Histonet] bone saws? Greeti

RE: [Histonet] gram stain controls

2008-11-26 Thread Walzer Susan
We take two pieces of fresh tissue (umbilical cord works well) to micro and have them grow positive and negative on individual pieces. After a few days we fix the tissue and embed them together. Works great. PS we do the same when we need fungus controls. -Original Message- From: [EMAIL

RE: [Histonet] RE: Is it Formalin-Without Smelling It?

2008-11-10 Thread Walzer Susan
You need to send the bottle back to where it originated. They are the ones who are responsible for correctly labeling hazardous solutions. From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of [EMAIL PROTECTED] Sent: Friday, November 07, 2008 3:59 PM To: Smith Wanda; h

RE: [Histonet] Does anyone have any ideas on the use of softenerondecal blocks. Does this help 'soften' them? If so how?

2008-10-07 Thread Walzer Susan
We face the block and put it in a dish with rapid decal solution. For tough blocks like uterus we soak them in a solution of water, ammonia(which is great for bloody tissue) and liquid soap. -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PROTECTED] On Behalf Of Laurie Reilly Sen

RE: [Histonet] Isopropyl vs Ethanol

2008-10-06 Thread Walzer Susan
We don't use it for processing but we use it at the end of the stain set up and coverslip out of it using Clearium mounting medium (it is misable with alcohol). We did it to keep out fingers out of xylene as it is a neurotoxin. -Original Message- From: [EMAIL PROTECTED] [mailto:[EMAIL PRO