Re: [Histonet] Price for preparing IHC slides

2014-02-03 Thread Will Chappell
A lab I used in Southern California charged $35 per stain for most antibodies. Sent from my iPhone On Feb 3, 2014, at 1:03 PM, Ann Specian thisis...@aol.com wrote: Can anyone tell me the average cost for preparing an IHC slide? thanks, Ann

Re: [Histonet] IHC-start-up

2014-01-23 Thread Will Chappell
you customize everything. I would suggest an OLD Dako or biocare's IntelliPath. Both have their quirks but they have the openness you require. William Chappell Sent from my iPhone On Jan 23, 2014, at 11:49 AM, Erin Sarricks esarri...@gmail.com wrote: Hi all- Our histopathology lab

Re: [Histonet] Processing:

2014-01-13 Thread Will Chappell
This depends on so many different factors, however, I prefer a frequent rotation over a complete change. Do what is best for your tissue! Sent from my iPhone On Jan 13, 2014, at 9:46 AM, Jb craiga...@gmail.com wrote: I have one tech telling me that when the entire processor is changed the

Re: [Histonet] New CPT code for IHC

2013-11-19 Thread Will Chappell
88343 refers to subsequent antibodies placed on the same slide such as multiplex staining or the PIN-4. Hope that helps. Will Chappell, HTL(ASCP), QIHC Sent from my iPhone On Nov 19, 2013, at 8:31 AM, Leann M. Murphy lmurp...@aultman.com wrote: How will everyone be handling the new CPT

Re: [Histonet] Specimen collection/transportation

2013-11-18 Thread Will Chappell
labelled, and appropriate SOPs in use on the floor, usually to include a spill kit. I wrote the procedures for the floor units, but it is their responsibility to be in compliance. Will Chappell, HTL(ASCP) Sent from my iPhone On Nov 18, 2013, at 12:02 PM, LeAnn Lang ll...@aipathology.com wrote

Re: [Histonet] Specimen collection/transportation

2013-11-18 Thread Will Chappell
And we just passed CAP with zero deficiencies. Sent from my iPhone On Nov 18, 2013, at 12:06 PM, Will Chappell cha...@yahoo.com wrote: This to me seems very odd. Almost exclusively specimens are sent to my lab in formalin. Placentas are usually sent fresh simply because of their size

Re: [Histonet] Histo arcania ... Reticulum vs Reticulin

2013-11-07 Thread Will Chappell
Reticulin is a type of fiber in connective tissue composed of type III collage secreted by reticular cells. Reticulum pertains to the endoplasmic reticulum, the second chamber of the alimentary canal of a ruminant animal, or (less frequent) the plural of reticular cell. Will Chappell Sent

Re: [Histonet] RE: Histo arcania ... Reticulum vs Reticulin

2013-11-07 Thread Will Chappell
Seems like our four-histotechs were the worst offenders for misuse. I believe Barry is right. A retic stain is a silver stain of a reticulum matrix of reticulin fibers (produced, of course, by reticular cells). Sent from my iPhone On Nov 7, 2013, at 4:35 PM, Morken, Timothy

Re: [Histonet] Height detail

2013-09-26 Thread Will Chappell
None according to cap. Maybe something from OSHA. Must maintain a certain distance from ceiling (8-12 inches I think) and must be up off floor (if stored in lab). They must be safe, however that is subjective. Will Chappell, HTL(ASCP) QIHC And available. Sent from my iPhone On Sep 26, 2013

Re: [Histonet] unsubscribe for NSH

2013-09-18 Thread Will Chappell
Oh dear lord. Not Colleen!! Why man hath wrought?? Sent from my iPhone On Sep 18, 2013, at 11:42 AM, Colleen Forster cfors...@umn.edu wrote: ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

[Histonet] Histologist Available for Southern California

2013-09-03 Thread William Chappell
at the chance for a bench position. He is looking for anything in San Diego, Riverside, Los Angeles, or San Bernardino county. If anybody, including temp recruiters, has anything, please contact me and I will forward it to my friend. Thanks, William Chappell, HTL(ASCP), QIHC

Re: [Histonet] picric acid paranoia

2013-06-04 Thread Will Chappell
Ship them as you would any biological test samples. No problems here. Sent from my iPhone On Jun 4, 2013, at 5:52 AM, Smith, Allen asm...@mail.barry.edu wrote: Picric acid bound to collagen is not an explosion hazard. Even if it were, the surrounding paraffin wax would cushion the picric

Re: [Histonet] fungal stain for frozen sections

2013-05-16 Thread Will Chappell
Will Chappell CHOC children's hospital. Sent from my iPhone On May 16, 2013, at 9:00 AM, wrg...@aol.com wrote: Hi, I work in a Surgical Pathology frozen section lab outside the OR rooms. Normally we only perform an HE on the frozens. Now we are looking for a fast and inexpensive stain

Re: [Histonet] Mohs histology hourly rate

2013-05-15 Thread Will Chappell
I would expect around $25 in twin cities and between $20 and $23 outside. For MOHs with 10-15 years experience. You could find higher wages, but those would be few and far between. Sent from my iPhone On May 15, 2013, at 5:16 AM, Jaclyn Pitts pitts.jac...@gmail.com wrote: Good Question. I

Re: [Histonet] Mohs histology hourly rate

2013-05-14 Thread Will Chappell
Location matters. Where? Sent from my iPhone On May 14, 2013, at 6:10 PM, rook...@comcast.net wrote: Hello, I was wondering if anyone knows what the going rate is for a Mohs histologist (Hourly). I have 12 years experience as a histologist doing routine histology and just recently got a

Re: [Histonet] Medical Director credentials on reports

2013-01-10 Thread Will Chappell
Just passed CLIA. It is a CLIA requirement. Not sure about CAP. Will Chappell CHOC Children's Sent from my iPhone On Jan 10, 2013, at 5:45 AM, histot...@imagesbyhopper.com wrote: Does anyone know, and maybe have the regs, whether either the Joint Commission or the CAP *require

Re: [Histonet] chemical disposal

2012-11-28 Thread Will Chappell
Chemical hazardous waste. Sent from my iPhone On Nov 28, 2012, at 12:44 PM, Cynthia Pyse cp...@x-celllab.com wrote: Quick question for Histoland. I am having a debate about DAB disposal. Our general manager ( non lab background) insists that the liquid DAB can go into a biological hazardous

Re: [Histonet] Help! Liver mistakenly processed in paraffin (had to be in OCT instead)!

2012-11-13 Thread Will Chappell
Nope, sorry. All your fat is dissolved. Sent from my iPhone On Nov 13, 2012, at 8:52 AM, z o n k e d zon...@gmail.com wrote: Hello Histonetters, First time writer, long time reader. I'm a newbie tech in academia and I was given a simple task which I think I pretty much screwed up. I

Re: [Histonet] Block/Slide placeholders

2012-10-31 Thread Will Chappell
Th product comes unassembled from any vendor, office supply store, paper store, or side of box. Just apply scissors as appropriate. Sent from my iPhone On Oct 31, 2012, at 6:58 AM, Elizabeth Cameron elizabeth.came...@jax.org wrote: Does anyone know where I can get the little cardboard tabs

Re: [Histonet] Number of blocks

2012-10-25 Thread Will Chappell
In fact, can you share them with all of histonet? Sent from my iPhone On Oct 25, 2012, at 7:33 AM, Hannen, Valerie valerie.han...@parrishmed.com wrote: Rene, I have been asked in the past about productivity in our department. Can you share those articles with me as well? Thanks!!

Re: [Histonet] voice recognition and synoptic reporting

2012-09-13 Thread William Chappell
with VoiceBrook. Just my opinion. Will Chappell CHOC Children's AP Supervisor On Sep 13, 2012, at 7:49 AM, Hutton, Allison ahut...@dh.org wrote: We have been asked to look at changing our pathology reports over to the synoptic report format. I was wondering if anyone could provide me some

[Histonet] Attention Vendors

2012-09-05 Thread William Chappell
again separately. Thanks so much, William Chappell HTL(ASCP)QIHC Anatomic Pathology Supervisor CHOC Children's ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] VoiceBrook

2012-09-05 Thread William Chappell
a bit more talk as they self-proclaim they are the ONLY real solution for pathology transcription. William Chappell HTL(ASCP)QIHC Anatomic Pathology Supervisor CHOC Children's ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http

Re: [Histonet] Proteolytic enzyme pretreatment before immunostaining

2012-07-31 Thread William Chappell
) for each antibody. One last thing, if you know more about the nature of the antigen used to create the antibody, you may be able to predict the required pretreatment -- talk to the primary investigator. Will Chappell, HTL(ASCP)QIHC Anatomic Pathology Supervisor Children's Hospital of Orange

Re: [Histonet] Billing IHC on MOHS

2012-06-19 Thread William Chappell
twice the work? Will Chappell HTL(ASCP), QIHC On Jun 19, 2012, at 9:15 PM, Kim Donadio wrote: Great team work! Job well done and a absolute answer is given. Thank you From: Carol Torrence ctorre...@kmcpa.com To: 'Kim Donadio' one_angel_sec...@yahoo.com

Re: [Histonet] Unregistered techs

2012-05-24 Thread William Chappell
. We don't do this simply because it is a good paycheck. We are histologists because we are professionals who choose this career. You may be going to a job cutting slides (which is great and necessary), but we are enjoying our life. Will Chappell, HTL (ASCP), QIHC, MBA and histologist by choice

Re: [Histonet] IHC ?

2011-09-15 Thread William Chappell
Excellent questions, I get it all the time. Overnight, in buffer, in the fridge will be ok. Do not leave in water as that will obscure morphology. Will On Sep 15, 2011, at 12:40 PM, sdys...@mirnarx.com wrote: Hello all, So I just realized I am out of my block after I have done HIER.

RE: [Histonet] Immuno's

2011-04-11 Thread William Chappell
specialist or TSR) out to help you in person. William Chappell, HTL(ASCP)QIHC IHC Development Specialist CellNetix Pathology Laboratories (503) 358-9567 www.cellnetix.com Our expertise...your peace of mind --- On Mon, 4/11/11, Rathborne, Toni trathbo...@somerset-healthcare.com wrote: From

Re: [Histonet] uh...

2011-04-07 Thread William Chappell
Sounds like your dehydrating alcohol is not dehydrating completely. I would switch out the 100%'s and the clearite. Either they got wet during the course of the day or someone made an oops when changing the stain line. Hope that helps. Will Sent from my iPhone On Apr 7, 2011, at 2:06 PM,

Re: [Histonet] uh...

2011-04-07 Thread William Chappell
just thought of, I never used clearite for coverslipping, even in labs that primarily used clearite -- I always finished up in Xylene. Will --- On Thu, 4/7/11, William Chappell cha...@yahoo.com wrote: From: William Chappell cha...@yahoo.com Subject: Re: [Histonet] uh... To: sgoe

Re: [Histonet] RE: Pin4cocktail negative controls

2011-02-21 Thread William Chappell
negative control serum. The universal component typically denotes use with rabbit and mouse antibodies. Will Chappell, HTL(ASCP)QIHC Histologist at large Sent from my iPhone On Feb 21, 2011, at 8:07 AM, Carol Bryant cb...@lexclin.com wrote: I would like that information also. Thank you, Carol

[Histonet] Biogenex

2010-08-12 Thread William Chappell
I heard a rumor that Biogenex was recently purchased. Can anyone confirm or deny said rumor? Will ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet