Re: [Histonet] Erroneous result

2019-11-07 Thread William Shipley via Histonet
? To further complicate the scenario, the original report may have gone to a clinician who is not authorized to see the results on the proper patient. Sending them an amended report which identifies the proper patient might constitute a HIPAA violation. William Shipley Schuyler House

Re: [Histonet] Productivity Tracking

2019-07-07 Thread WILLIAM DESALVO via Histonet
and productivity goals MUST be strongly linked or the patient suffers!!! William DeSalvo From: Jay Lundgren Sent: Sunday, July 7, 2019 1:20:56 PM To: jasonhause...@gmail.com Cc: WILLIAM DESALVO; Pairan, Kelly; histonet@lists.utsouthwestern.edu Subject: Re

Re: [Histonet] Productivity Tracking

2019-07-05 Thread WILLIAM DESALVO via Histonet
I suggest you use slides created for the microtomy minimum standard. Not all blocks are created equal. A good target is 30 slides per hour, for mixed specimens. If all specialty, then adjust from the 30. William DeSalvo From: Pairan, Kelly via Histonet Sent

Re: [Histonet] Histonet - Molecular Biology ASCP

2019-05-31 Thread WILLIAM DESALVO via Histonet
If you have the HTL, you are eligible to take the MB. You can also track with education and OJT for one year in molecular lab. William DeSalvo From: Joe Myers via Histonet Sent: Friday, May 31, 2019 4:15:01 PM To: HistoNet Subject: Re: [Histonet] Histonet

Re: [Histonet] Tissue Tek TEC 6 Embedding Centre

2019-05-30 Thread WILLIAM DESALVO via Histonet
Check with the manufacturer as many in the pathology space are moving to placing 10 use and no longer supporting. As this continues to become more prevalent, the refurbish companies will continue to support. This is similar to the support of some clinical instruments. William DeSalvo

Re: [Histonet] Suggestions on a used microtome

2019-04-23 Thread WILLIAM DESALVO via Histonet
Contact Rankin for reliable refurbished instruments. I am partial to retracting microtones. Best to work with a company that provides quality William DeSalvo From: Craig via Histonet Sent: Tuesday, April 23, 2019 12:27:22 PM To: histonet@lists.utsouthwestern.edu

Re: [Histonet] Fridge temp

2019-03-26 Thread WILLIAM DESALVO via Histonet
Simple, revalidate or pitch William DeSalvo From: MARY ANN via Histonet Sent: Tuesday, March 26, 2019 4:09:01 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Fridge temp Let's say, hypotheticaly, if you discover your fridge with all you antibodies

Re: [Histonet] Really Small acid cabinet?

2019-03-07 Thread William Ahlfeld via Histonet
Check out Uline part #H-4174 Sent from my iPad ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] AB

2018-11-29 Thread WILLIAM DESALVO via Histonet
Helen, I have experience with both. Feel free to contact me directly with questions or we can arrange a call. More than happy to help William DeSalvo wdesalvo.cac@outlook. Com 480-622-1337 William DeSalvo From: Helen via Histonet Sent: Thursday, November 29

Re: [Histonet] AFB STAIN

2018-10-04 Thread WILLIAM DESALVO via Histonet
I completely agree with Tony. Not likely to see any sloughing off (shedding) of organisms from FFPE tissue blocks. William DeSalvo From: Tony Henwood (SCHN) via Histonet Sent: Thursday, October 4, 2018 1:54 PM To: adesupo2...@hotmail.com; Laurie Colbert Cc

Re: [Histonet] Temperature for Storing Slides and Blocks

2018-09-05 Thread WILLIAM DESALVO via Histonet
I do not know of a standard, but you should make sure the storage temp is 5-10 degrees lower(maybe more depending on fluctuations in temp for the area). That would be < 120F degrees. Request the location Facilities department understands you need temperature control William DeSa

Re: [Histonet] Davidson

2018-07-30 Thread WILLIAM DESALVO via Histonet
lly, a quick verification study on multiple tissue samples before risking diagnostic samples. Hope that helps. William William DeSalvo From: Azam, Muhammad via Histonet Sent: Monday, July 30, 2018 10:55 AM To: histonet@lists.utsouthwestern.edu Subject: [Histo

Re: [Histonet] Paperwork

2018-07-27 Thread WILLIAM DESALVO via Histonet
be multiple responses to facilitate your change. William William DeSalvo From: Campbell, Tasha M. Sent: Friday, July 27, 2018 11:00 AM To: WILLIAM DESALVO; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] Paperwork I am a little confused about your batch

Re: [Histonet] Paperwork

2018-07-27 Thread WILLIAM DESALVO via Histonet
. All of your manual tracking process is now electronic. Do update all SOP’s and note date of change in process. William William DeSalvo From: Campbell, Tasha M. via Histonet Sent: Friday, July 27, 2018 8:41 AM To: histonet@lists.utsouthwestern.edu Subject

Re: [Histonet] Coronary or Muscular Artery Histology Textbooks

2018-07-26 Thread WILLIAM DESALVO via Histonet
Stevens and Lowe, Histology, 4th edition, 2015. Good human anatomy and descriptive William DeSalvo From: Vivian Hou via Histonet Sent: Thursday, July 26, 2018 11:40 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] Coronary or Muscular Artery

[Histonet] Quest

2017-06-22 Thread William Shipley via Histonet
samples? We are in the Los Angeles area. William Shipley Schuyler House ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] I am having trouble with testis tissue slide preps.

2016-12-20 Thread William Weber via Histonet
I am having trouble with testis tissue slide preps. I am fixing and embedding rodent testis in paraffin, however the tissue is not embedding completely in the wax. It falls out of the block and when it stays in the block the microtome will chunk it off. My Protocol 1. fixation in Bouin’s

Re: [Histonet] Histonet Digest, Vol 157, Issue 1

2016-12-01 Thread WILLIAM JACKSON via Histonet
Can someone please give me some tips in using an automatic microtome wheel, were the advance wheel keeps movivg eith forward of backward until you take your hand off of it, any little tip is useful. Meyosha Jackson HT(ASCP) > > On December 1, 2016 at 1:00 PM

Re: [Histonet] Water for H Stainers?

2016-09-27 Thread WILLIAM DESALVO via Histonet
in running tap water will remove most of the excess mordant giving sharp blue nuclear staining. If you want sharper and better defined As my good friend Skip Brown states, a good H stain is "balance of coloration". Understand the chemistry and you control the balance. William DeSalvo, B

Re: [Histonet] No More Blog Posts -- Over and Out!

2016-05-02 Thread WILLIAM DESALVO via Histonet
Date: Mon, 2 May 2016 20:15:38 + > > As is yours. Just can't keep that dictatorial aspect out of your charming > consultant two cents worth. > > Steve Pinheiro > > > -Original Message- > From: WILLIAM DESALVO via Histonet [mailto:histonet@lists.utsouthweste

Re: [Histonet] No More Blog Posts -- Over and Out!

2016-05-02 Thread WILLIAM DESALVO via Histonet
Your response is WAY out of line. Keep it professional. Sent from my Windows Phone From: Manfre, Philip via Histonet Sent: ‎5/‎2/‎2016 12:08 PM To: Rene J Buesa Cc:

Re: [Histonet] Medical/health related post

2016-04-28 Thread WILLIAM DESALVO via Histonet
Again, why the non-Histo post. Take this to another source. I do not understand why Dr. Raff has not been removed from this list serve. This is a valuable site for histotechnolgy related issues, please let us keep it that way. Sent from my Windows Phone From:

Re: [Histonet] Passing the HTL

2016-04-26 Thread WILLIAM DESALVO via Histonet
Congratulations! Well done and now the fun begins. Sent from my Windows Phone From: Terri Braud via Histonet Sent: ‎4/‎26/‎2016 10:58 AM To: 'histonet@lists.utsouthwestern.edu'

Re: [Histonet] Blog Post Not lab related

2016-04-14 Thread WILLIAM DESALVO via Histonet
IMO, this is a list serve not an advertising space. Keep blog posts and other advertising off the list. Keep the exchange about histotechnology. Plenty of interweb space for other communication. Dr. Raff and everyone is always welcome to be part of the discussion and help get answers to those

Re: [Histonet] Processing issues

2016-03-22 Thread WILLIAM DESALVO via Histonet
Not knowing what issues you have, I suggest you look to your samples for processing. Reduce thickness and the shorter times will work. 2-2.5 mm thick. Standardize fixation before placing in tissue processor. What time is the processor started and what time does the tech remove? You may be able

Re: [Histonet] automatic stainers and tissue processors

2016-02-29 Thread WILLIAM DESALVO via Histonet
I suggest you take the opportunity to find the instruments that fit your needs. Are going to rapid process, what types of tissue samples do you have, what do you want to improve? The histology lab is changing and you should look 5+ years down the road. I would not want you to be pigeon holed

Re: [Histonet] Bar Code and Tracking

2016-02-18 Thread WILLIAM DESALVO via Histonet
I believe it will be difficult to afford the cost of Co-Path in your small lab area, unless you can attach to a bigger hospital information system contract. I suggest you look to companies that have developed products for the small to medium sized histology labs. Sent from my Windows Phone

Re: [Histonet] Storage of Unstained Slides

2016-02-01 Thread WILLIAM DESALVO via Histonet
I would not heat dry unstained as heat drying removes the paraffin and cells/tissue in no longer protected by the paraffin. Drying slides is a process to remove the sate trapped between the section and glass. Heat drying can speed that process and is also helpful in "baking" tissue to slide to

Re: [Histonet] changing tissue processors

2016-01-11 Thread WILLIAM DESALVO via Histonet
I would suggest by cassette numbers. It will take some data collection, but once you settle on the number of cassettes, then you will have the process in control. The actual number will depend on the tissue types placed in the cassettes and the amount of blood, lipids and other elements

[Histonet] NSH Financial and Tax Statements

2016-01-07 Thread WILLIAM DESALVO via Histonet
As a due paying member of NSH, I ask all members and anyone interested in Histotechnology request from NSH office to see the Financial and Tax Statements for 2013 / 2014. This is public information. We should all know where our dues money, money from educational activities and vendor money from

Re: [Histonet] Help with causes or theory behind failure of nuclear counterstaining after IHC!

2016-01-04 Thread WILLIAM DESALVO via Histonet
Maria, I. Think you may have a pH issue. High pH results in reduction of protons, H+, effects dye structure and can cause light to no staining after bluing. If you are using hematoxylin w/ aluminum, most popular, decreased pH = decreased intensity. Acid breaks the Al+3. Check your ph throughout

Re: [Histonet] Collodian bag for cell blocks

2016-01-04 Thread WILLIAM DESALVO via Histonet
What method are you using? Making your own coated tubes, UCSF or MD Anderson. Are you purchasing coated tubes? Once the tube is coated, dried (using hood) and filled w dH2O, there should be no odor. Always work w/ collodion under the hood. Ethyl ether is main component. Once in cassette and

Re: [Histonet] Best solution for surface decalcification of paraffin block

2015-12-16 Thread WILLIAM DESALVO via Histonet
I do no recommend surface decal, but if you must use a formic acid product. Rinse the block several times to remove decal before cutting a section. Always better to go to the source and adjust time in decal and method for determining end point before processing. Sent from my Windows Phone

Re: [Histonet] Workload

2015-12-08 Thread WILLIAM DESALVO via Histonet
I measure productivity by unit per task (specimens, blocks, slides). There is no right or wrong, but there are multiple tasks associated w/ a CPT code. I suggest you talk to you management or finance and find out what they use to measure productivity. Using their method/units allows for roll up

Re: [Histonet] Rapid tissue Programs

2015-11-24 Thread WILLIAM DESALVO via Histonet
First suggestion is to remove the sponge. There will be carry over on biopsy/rapid tissue processor schedules. The sponges do require longer times to drain and exchange liquids. Your fixation times must be >6 hours. Make sure you are not applying too much heat to processing. Small biopsies are

Re: [Histonet] Xylene and Formalin substitutes

2015-10-21 Thread WILLIAM DESALVO via Histonet
Contact Ada Feldman, Anatech Ltd. Best resource. Sent from my Windows Phone From: Bharti Parihar via Histonet Sent: ‎10/‎21/‎2015 3:49 PM To: histonet@lists.utsouthwestern.edu

Re: [Histonet] dictation systems

2015-09-17 Thread WILLIAM DESALVO via Histonet
At our lab, we have used a digital server system called Fusion. I suggest you go digital and then consider if you can also move to voice recognition and activation for efficiency. There are so many more options now as compared to the tape systems. Sent from my Windows Phone

Re: [Histonet] HE Stainer Question

2015-05-12 Thread WILLIAM DESALVO
and will the instrument help or hinder a LEAN workflow? There are many choices that will meet your basic needs, but which one meets your essential needs? William DeSalvo, BS HTL(ASCP) From: sim...@upmc.edu To: ro...@labcorp.com; pat...@gmail.com; histonet@lists.utsouthwestern.edu Date: Tue, 12 May

Re: [Histonet] IHC billing question

2015-04-30 Thread WILLIAM DESALVO
We have to manually review the IHC billing also and continue to audit. It took billing and IT three months to create the logic to automate billing for a specimen and account for combination of there being the possibility of 88341, 88342 88344 and the proper combinations. Sent from my iPhone

Re: [Histonet] RE: Nuclear Artifact

2015-04-29 Thread WILLIAM DESALVO
What type of tissue cassette is being used? What type of insert or wrap is used. If one cassette processes correctly and the next to it does not, hard to say tissue processor is causing issue. Sounds like a water problem and it could be water trapped in cassette. Check the rest of you process

Re: [Histonet] Over- decalcified tissue

2015-04-29 Thread WILLIAM DESALVO
I would suggest not allowing decalcification to be extended. Better to leave in fixative until you can control the time of endpoint of decalcification. What type of decal solution are you using? I am not a big fan of trying to adjust the chemistry of the stain to compensate for over

Re: [Histonet] BS in Histotechnology

2015-04-23 Thread William J. O'Connor III
I have monkeys where I work, cynos. We tried to train them to not bite our fingers off - I wouldn't even try to teach them to use a microtome. -Original Message- From: Michael Ann Jones mjo...@metropath.com To: Stedman, Nancy nancy.sted...@buschgardens.com; Mark Turner

Re: [Histonet] prevent wrinkles when cutting

2015-04-20 Thread WILLIAM DESALVO
If you process properly, no over dehydration, there is no reason to soak. Keeping block cool will help in cutting. Sharp, clean blade a must. Correct water bath temp and time are the most critical factors, after cutting, to produce a wrinkle free section. Sent from my iPhone On Apr 20,

Re: [Histonet] IHC and oven temperature

2015-04-20 Thread WILLIAM DESALVO
Dry heat compared to wet heat. Do not dry your slides at high heat. You are removing water trapped between slide and paraffin section. Antigen retrieval is an entirely different process. So not try to combine the two processes Sent from my iPhone On Apr 20, 2015, at 8:48 AM, Preiszner,

Re: [Histonet] FW: Release of blocks to research facilities

2015-03-17 Thread WILLIAM DESALVO
There are other issues, besides CAP, that you should consider when releasing blocks. 1. A block is a medical record. Has the patient consented to release for research? 2. If the block is released prior to 14 days discharge and there are any charges, the charge must be sent to your hospital.

Re: [Histonet] Embedding Question

2015-03-12 Thread WILLIAM DESALVO
Cooling the paraffin and then re melting will not affect the tissue, unless the combined heated, liquified state period becomes extended. Cooling the paraffin is a great protector of the tissue, no different than what you have with a completed block. Be cautious at how fast and at what

Re: [Histonet] Troponin is biomarker of heart attacks

2015-02-05 Thread William J. O'Connor III
troponin I is the only troponin assay to show cardiac muscle injuty. -Original Message- From: Jorge A. Santiago-Blay blayjo...@gmail.com To: Histonet Histonet@lists.utsouthwestern.edu Sent: Thu, Feb 5, 2015 3:02 pm Subject: [Histonet] Troponin is biomarker of heart attacks Dear

Re: [Histonet] Block Counts

2015-01-12 Thread WILLIAM DESALVO
inc) that allows you to set work pace and creates a corresponding quality measure. William DeSalvo william.desa...@sonoraquest.com 602-768-3692 Sent from my iPhone On Jan 12, 2015, at 4:05 PM, Morken, Timothy timothy.mor...@ucsf.edu wrote: I agree with Diana, I found we had over a dozen

Re: [Histonet] RE: Angle of Wrist/Hands Using Microtome?

2014-12-08 Thread WILLIAM DESALVO
OSHA states maintain neutral wrist and arm when working; work with their wrists in a neutral or straight William DeSalvo william.desa...@sonoraquest.ccom 602-768-3692 Sent from my iPhone On Dec 8, 2014, at 2:41 PM, Blazek, Linda lbla...@digestivespecialists.com wrote: My two cents... # 2

Re: [Histonet] fixative

2014-11-25 Thread WILLIAM DESALVO
What are you going to accomplish? Alcohol will work as a fixative and dehydration solution. Less of a disposal issue. William DeSalvo william.desa...@sonoraquest.ccom 602-768-3692 Sent from my iPhone On Nov 25, 2014, at 8:30 AM, Schade, Adelle a_sch...@conradweiser.org wrote: Hello, I am

Re: [Histonet] Flames at embedding centers

2014-11-18 Thread William J. O'Connor III
The open flames were great because they incinerated any residual tissue fragments, like from EMCs. Hardy ever had cross contamination back in those days. Lab fires, yes - I remember one in the late 1960's at St. Elizabeth Hospital in Chicago. (Just heard about it, wasn't there at the time)

[Histonet] IHC general fixation

2014-11-12 Thread William J. O'Connor III
I'm looking for input on the latest theories of duration of fixation for routine IHC. In the past, it was thought that tissues should be removed from 10% NBF after a max of 72 hours, and if not immediately processed, transferred to 70% ethanol until processed to retain antigenicity. What

[Histonet] Paraffin Used

2014-10-23 Thread WILLIAM DESALVO
What paraffin do you use in your lab? Do you use different type for processing and embedding? Looking to investigate the top used paraffins for a workshop I am putting together. Sent from my iPhone ___ Histonet mailing list

Re: [Histonet] slide id

2014-10-03 Thread WILLIAM DESALVO
We use Vantage Quality and Tracking system and the tech is captured for embedding, microtomy and staining Sent from my iPhone On Oct 3, 2014, at 12:55 PM, Tapper, Sheila J. sheila.tap...@essentiahealth.org wrote: We have our techs fast process the slides that they cut - so the tech is

Re: [Histonet] slide id

2014-10-03 Thread WILLIAM DESALVO
, Rathborne, Toni toni.rathbo...@rwjuh.edu wrote: How do you like Vantage? Have you experienced any problems with it, and is it easy to use? -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM

Re: [Histonet] slide id

2014-10-03 Thread WILLIAM DESALVO
generate new barcoded labels and affix them to the materials prior to sending them out (for tracking purposes)? Thanks, Brian -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of WILLIAM

[Histonet] Whole rat head histology

2014-09-17 Thread William J. O'Connor III
I have a fun project - processing a whole rat head, skull with brain, for serial sections to see a brain/skull congenital deformity.Anyone have any experience with this? I'd love it if you could share your protocol. Paraffin or plastic? Jackie O'

RE: [Histonet] (no subject)

2014-09-10 Thread WILLIAM DESALVO
University of Michigan MLabs William DeSalvo, BS HTL(ASCP) From: ttro...@petersonlab.com To: histonet@lists.utsouthwestern.edu Date: Wed, 10 Sep 2014 12:22:55 -0500 Subject: [Histonet] (no subject) I was wondering what facility labs are sending nerve and muscle biopsies

Re: [Histonet] On the lighter side...

2014-08-08 Thread William J. O'Connor III
44 years. HT(ASCP) 5483. Took a year off to run a gas station in the early 70's. Worked in Chicago, Memphis, Jacksonville, FL, San Diego, Honolulu. Lots of good times and great people. Jackie O' -Original Message- From: Rena Fail renafa...@gmail.com To: Jim Burchette

Re: [Histonet] On the lighter side...

2014-08-08 Thread William J. O'Connor III
44 years. HT(ASCP) #5483. Worked in Chicago, Memphis, Jacksonville, FL, San Diego, Honolulu, back to Chicago area now. Lots of fun, met many wondrous people along the way. Jackie O' ___ Histonet mailing list Histonet@lists.utsouthwestern.edu

Re: [Histonet] Protocol for decalcifying and processing large bone section.

2014-08-05 Thread William J. O'Connor III
Is there a need to decalcify the femoral head intact? We typically cut large, unfixed veterinary bones with an Exakt band saw into 5 mm slices prior to fixation and decalcification. This ensures the bones are well fixed prior to decalcification. We decalcify the selected slices for about

RE: [Histonet] Paraffin blocks

2014-07-10 Thread WILLIAM DESALVO
for your blocks and cutting waste. William DeSalvo, BS HTL(ASCP) From: vriv...@westderm.com To: bcoo...@chla.usc.edu; jfra...@hotmail.com; histonet@lists.utsouthwestern.edu Date: Thu, 10 Jul 2014 20:37:39 + Subject: RE: [Histonet] Paraffin blocks CC: You would think, especially when

Re: [Histonet] Should I leave histology world

2014-06-03 Thread WILLIAM DESALVO
Do not give up and try not to blame a process or someone else for your lack of developed skill at microtomy, but dedicate yourself to personal improvement. You have 6+ years invested in a career, and if your reasons for embarking on that career remain, then bear down and find a way to improve

Re: [Histonet] Pathology Report Templates

2014-04-28 Thread William Shipley
that the advent of patients having the right to get their lab report would be an argument against the medical director being listed as this is probably not the person that the patient should be calling for an explanation of their report. This is just my opinion, of course. William Shipley Schuyler

RE: [Histonet] Recommendations cryostat

2014-04-21 Thread WILLIAM DESALVO
quality. William DeSalvo, BS HTL(ASCP) From: hi...@skm.org.pk To: histonet@lists.utsouthwestern.edu Date: Mon, 21 Apr 2014 14:01:36 + CC: tahseen0...@gmail.com Subject: [Histonet] Recommendations cryostat Hi All, We are going to buy cryostat so opinion required CM 1950 LEICA verses

Re: [Histonet] Negative Reagent Control in Diagnostic IHC Testing

2014-04-14 Thread WILLIAM DESALVO
Thanks for the great information. Downloaded and being shared w/ my lab. Sent from Windows Mail From: Cartun, Richard Sent: ‎Monday‎, ‎April‎ ‎14‎, ‎2014 ‎4‎:‎04‎ ‎PM To: histonet For those of you who are interested, I have an Editorial in the March issue (Volume 22, Number 3) of

RE: [Histonet] Telepathology

2014-04-10 Thread WILLIAM DESALVO
Association (DPA) web site. I am a member and this group is dedicated to the advancement and education of WSI. Check out the web site William DeSalvo, BS HTL(ASCP) To: histonet@lists.utsouthwestern.edu From: mtitf...@aol.com Date: Thu, 10 Apr 2014 08:14:23 -0400 Subject: [Histonet] Telepathology

[Histonet] Frozen sectioning whole rat eyes

2014-03-26 Thread William J. O'Connor III
Hello Histoland! A colleague of mine is trying to section unfixed whole rat eyes in OCT. She is getting a lot of tearing at the top of her section. Lens and optic nerve on the horizontal plane. Any suggestions, recommendations would be great! Jackie

RE: [Histonet] What is a level?

2014-03-17 Thread WILLIAM DESALVO
upon by the pathologist and microtomist. This takes the guess work out of the process. once you have a specific description, make sure this is added to your SOP. William DeSalvo, BS HTL(ASCP) From: bgapin...@pathgroup.com To: histonet@lists.utsouthwestern.edu Date: Mon, 17 Mar 2014 21:19:40

Re: [Histonet] Interfacing with Meditech Magic

2014-03-16 Thread WILLIAM DESALVO
to a special staining slide. I suggest you might want to sit down and “map out’” (flow chart) your cutting and pathologist ordering process so that you can create the logical tracking process needed to work with your LIS. William DeSalvo BS, HTL(ASCP) Sent from Windows Mail From

RE: [Histonet] RE: Cornflaking artifact

2014-03-13 Thread WILLIAM DESALVO
Long time and high volume user of the Sakura Tape. Check the Xylene drops. We coverslip thousands of slides daily and have seen no isssue with the tape lots. Our experience shows some cornflaking when the slide does not receive enough xylens to wet the slide properly. William DeSalvo, BS HTL

RE: [Histonet] Decal and molecular testing

2014-03-11 Thread WILLIAM DESALVO
in 10% Formalin, time is dependant upon the size. Use stirring, heat, 50 degrees C, and pH 7.2-7.4. MOL-Decal 10 suggests 5-6 hrs for small biopsy bones and Immunocal 2-4 hrs. Try these and test at your site. Make sure you do a validation study to confirm results and use. Good luck William

RE: [Histonet] Clinical histology to Research histology

2014-02-04 Thread WILLIAM DESALVO
for funding to keep the job going. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting From: cda...@che-east.org To: histonet@lists.utsouthwestern.edu Date: Tue, 4 Feb 2014 09:49:32 -0500

Re: [Histonet] Gross lab seniors

2014-02-02 Thread WILLIAM DESALVO
is to set up a process and then regularly check. Sent from Windows Mail From: E. Wayne Johnson 朱稳森 Sent: ‎Saturday‎, ‎February‎ ‎1‎, ‎2014 ‎3‎:‎12‎ ‎PM To: WILLIAM DESALVO Cc: Vickroy, Jim, histonet Face velocity is simply the airflow rate in CFM divided by the area of the hood opening

RE: [Histonet] Gross lab seniors

2014-01-31 Thread WILLIAM DESALVO
on the room and the air exchange rate for the area. I have always felt the face velocity is most important to gross dissection personnel. There needs to be adequate draw away from the employee, no matter the physical conditions of the room. William DeSalvo, BS HTL(ASCP) Production Manager

RE: [Histonet] -80 degrees rationale

2013-11-20 Thread WILLIAM DESALVO
get below -80°C you will need cryoprotectors and when you use cryoprotectors, temperatures must be below −130°C and will go as low as −196°C (liquid nitrogen). Antibodies and proteins in solution are stable at −20°C. William DeSalvo, BS HTL(ASCP) Date: Wed, 20 Nov 2013 09:25:54 -0500 From: pat

RE: [Histonet] Specimens from surigical units

2013-11-20 Thread WILLIAM DESALVO
We use the hospital LIS. All specimens/cases are entered and ordered by Surgery and they are then received by Surgical Pathology. You are able to effectively track w/ pending lists and a full manifest/reconciliation process. William DeSalvo, BS HTL(ASCP) From: vickroy@mhsil.com

[Histonet] RE: Second Question of the Day: -80 degrees rationale

2013-11-20 Thread WILLIAM DESALVO
The only way to really know is to validate the temperature for the samples you want to store. The acceptable range must meet the performance of the unit and be in an acceptable range for any manufactureg materials stored in the freezer. All this will be tied up in your SOP. William DeSalvo

RE: [Histonet] Nail processing

2013-11-08 Thread WILLIAM DESALVO
for processing.Submit one cassette of representative section(s). Microtomy of SectionsUse Fisher Brand Burnt Orange Plus Slides.Cut two sections per slide.Air Dry then place in 60⁰ C oven for 2 hours.Allow to come to room temp before starting staining process. William DeSalvo, BS HTL(ASCP) Production

RE: [Histonet] Faxitron Xray digital imaging

2013-11-05 Thread WILLIAM DESALVO
. There is only one company that manufactures and sells the instrument: Faxitron Bioptics, LLC Tucson, AZ 877-910-0030 The are willing to bring a unit to your site for demonstration and you may be able to talk them into leaving for a evaluation. William DeSalvo, BS HTL(ASCP) From: allison.sc

RE: [Histonet] Specimen Tracking Systems

2013-10-31 Thread WILLIAM DESALVO
. And negotiate hard, the pricing is all over the place for the systems. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Board Member, Digital Pathology Association Owner/Consultant, Collaborative Advantage Consulting From: mro

RE: [Histonet] Oil red O

2013-09-09 Thread WILLIAM DESALVO
I believe the answer should be - Oil Red O is a brand name (not uncommon in the dye indutry) and represents the two dyes found in the compound, Solvent Red 27 and Sudan Red 5B William DeSalvo, BS HTL(ASCP) Date: Mon, 9 Sep 2013 13:32:08 -0700 From: rjbu...@yahoo.com To: adafeld

[Histonet] Histologist Available for Southern California

2013-09-03 Thread William Chappell
at the chance for a bench position. He is looking for anything in San Diego, Riverside, Los Angeles, or San Bernardino county. If anybody, including temp recruiters, has anything, please contact me and I will forward it to my friend. Thanks, William Chappell, HTL(ASCP), QIHC

RE: [Histonet] Requisitions

2013-08-21 Thread WILLIAM DESALVO
(taken from the requisition). There are many labs that are paperless during the technical process and I see more and more use of electronic requisitions and orders. William DeSalvo, BS HTL(ASCP) Chair, NSH Quality Management Committee Date: Wed, 21 Aug 2013 11:55:12 -0700 From: gmar

[Histonet] NSH Quality Management Committee

2013-08-20 Thread WILLIAM DESALVO
and Convention. William DeSalvo, BS HTL(ASCP) Chair, NSH Quality Management Committee ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

RE: [Histonet] Oven recommendations

2013-05-22 Thread WILLIAM DESALVO
. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting From: minnies...@hotmail.com To: histonet@lists.utsouthwestern.edu Date: Wed, 22 May 2013 13:55:46 + Subject: [Histonet] Oven

RE: [Histonet] Vantage and Prisma

2013-05-10 Thread WILLIAM DESALVO
I use Vantage and Sakura Prisma. The Sakura Prisma is not interfaced into Vantage. You place a bar-coded label on your HE slides at microtomy. We scan the slides after staining, case assembly. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management

RE: [Histonet] Re-use of plastic embedding molds

2013-05-09 Thread WILLIAM DESALVO
We tried using the disposable molds and they work for reusing 2-3 times( cracks started after just a few uses), but we found the cost much higher that using the metal molds and cleaning. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee

RE: [Histonet] RE: CAP standard GEN 61300

2013-05-06 Thread WILLIAM DESALVO
and maintains records. http://www.tutelamedical.com/ William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting Date: Mon, 6 May 2013 08:12:01 -0700 From: katelin09...@gmail.com

RE: [Histonet] cassette labelers

2013-04-30 Thread WILLIAM DESALVO
workflow, consider your specific needs and then ask each of the vendors to let you demo their units to see which one will work best to support your process. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant

RE: [Histonet] Tracking OR specimens

2013-03-18 Thread WILLIAM DESALVO
to reconcile cases and contact OR is a case has not been delivered by end of day. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting From: michelle.lamph...@childrens.com

RE: [Histonet] IHC validation

2013-03-15 Thread WILLIAM DESALVO
samples to the protocol and make adjustments, as necessary. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting From: lcolb...@pathmdlabs.com To: histonet@lists.utsouthwestern.edu Date

RE: [Histonet] Temperatures

2013-02-08 Thread WILLIAM DESALVO
wait until we have a questionable result before we check for quality. William DeSalvo, BS HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting Date: Fri, 8 Feb 2013 20:03:17 -0500 From: amosbro...@gmail.com

RE: [Histonet] No expiration dates on chemicals

2013-01-31 Thread WILLIAM DESALVO
. William DeSalvo, B.S., HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting Date: Thu, 31 Jan 2013 15:49:44 -0500 From: pat...@gmail.com To: jpiche-gro...@wtbyhosp.org Subject: Re: [Histonet] No expiration

RE: [Histonet] vacuum sealer

2012-12-30 Thread WILLIAM DESALVO
Cardinal Health - Kapac System, 18 inch sealer w/ special bags for storing formalin. Best system for Histo William DeSalvo, B.S., HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting Date: Sun, 30 Dec

RE: [Histonet] Histotechnician Salary 2

2012-12-19 Thread WILLIAM DESALVO
% of salary. William DeSalvo, B.S., HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting From: timothy.mor...@ucsfmedctr.org To: twheel...@mclean.harvard.edu; histonet@lists.utsouthwestern.edu Date: Wed, 19 Dec

RE: [Histonet] Bluing

2012-12-18 Thread WILLIAM DESALVO
with time. William DeSalvo, B.S., HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting Date: Tue, 18 Dec 2012 11:20:26 -0500 From: turke...@gmail.com To: histonet@lists.utsouthwestern.edu Subject

RE: [Histonet] Hematoxylin issue

2012-12-18 Thread WILLIAM DESALVO
for review. Most important is to find the cause, stop the problem and move forward. William DeSalvo, B.S., HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting Date: Tue, 18 Dec 2012 14:45:42 -0500 From

RE: [Histonet] Re: Cutting Blocks

2012-10-26 Thread WILLIAM DESALVO
blocks and slides. I may have added a bit more than 2 cents worth. William DeSalvo, B.S., HTL(ASCP) Production Manager-Anatomic Pathology Chair, NSH Quality Management Committee Owner/Consultant, Collaborative Advantage Consulting From: thigg...@cddmedical.com To: histonet

[Histonet] NSH Quality Management Forum

2012-10-13 Thread WILLIAM DESALVO
- October 20, 2012Location - Bethesda, MD Check out the link for all the details or call NSH office at: 443-535-4060 William DeSalvo, B.S., HTL(ASCP) Chair, NSH Quality Management Committee ___ Histonet mailing

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