[Histonet] Recent issues with picro sirius red staining (entire liver section become red, no yellow background)

2019-08-09 Thread abi jag via Histonet
Hello Histonetters,I am writing this to seek your help regarding a very recent problem that I am currently facing with Picro Sirius red staining of lab animal (mouse and rat) liver samples. I follow the procedure that was provided by John Kiernan in the histonet archives (please see below), whic

[Histonet] Troubleshooting Gomori's Trichrome Stain (Blue Collagen, Richard-Allen) staining

2019-07-25 Thread abi jag via Histonet
Dear histo experts,Please provide me with your valuable suggestions for the problem described below.Objective: Staining rat hearts (fibrosis) with Gomori's one step Trichrome Stain (Blue Collagen, Richard-Allen) to demonstrate the collagenProcedure: Paraffin sections of 4 micron thickness; adequ