[Histonet] LAMP-2 and adipophilin IHC on mouse(formalin fixed paraffin sections)

Great folks in histonet, Can any of you have done or having experience in IHC detection of LAMP-2 and Adipophilin on formalin fixed paraffin sections of mouse. I am working on differentiating phospholipidosis and neutral lipidosis in mouse tissues. please share your experience/protocol.

[Histonet] collagen I IHC detection in mouse pulmonary fibrosis

All, I would like to quantify collagen 1 in bleomycin induced lung fibrosis in mice. It will be highly appreciated if any of our histonetters will share their protocol for IHC staining of perfusion fixed(10% NBF) mouse fibrotic lungs. Thanks in advance Abi

[Histonet] staining of immature collagen in lung fibrosis models

Dear Histonetters, I am looking for hearing from our experienced histonetters regarding the possibility of staining of immature collagen fibres in animal models of lung fibrosis. I didn't find any histochemical method in the literature to stain the immature collagen. I would like to know

[Histonet] Luxol Fast Blue +PAS staining on rat spinal cord

Dear Fello histonetters, I am looking for demonstration of demyelination in rat spinal cord paraffin sections. A quick look into the literature revealed use of combination of luxol fast blue and PAS for demonstration of demyelination and myelin breakdown products. Anybody over here will

[Histonet] immunohistochemical staining of macrophages in rat mesentery adipose tissue reg

Dear Histonetters, I am requesting your valuable advice/suggestion on immunohistochemical staining of macrophages in rat mesentery adipose tissue. I would like to know the type of primary and secondary antibodies you are using(manufacturer details)and your preferable protocol etc. Thanks

[Histonet] collection, trimming and embedding of rat mesentery for vascular changes

Dear fellow histonetters, I would like to know from your experience with respect to collection, trimming and embedding of mesentery from rat. Our pathologists are interested in light microscopic examination of blood vessels of mesentery from rat. The objective is to get as many as blood

[Histonet] mouse heart trimming and sectioning_papillary muscle

Dear fellow histonetters, I would like to have your suggestions regarding trimming and sectioning of mouse heart. Our pathologists are interested in evaluating mouse ventricle papillary muscle for some lesions. I am not able to cut sections having papillary muscle in all sections.Some

[Histonet] rat kidney problems

Hello I need your advice regarding paraffin sections of rat kidney. Kidneys are collected, adequately fixed with 10 % NBF and undergone routine tissue processing in Sakura VIP 6 tissue processor.Sections are of 5 micron thickness. Strangely, in the HE stained sections, our pathologists are

[Histonet] Rat heart valve histology trimming and sectioning reg

Dear Histonetters, Our pathologists are interested in rat heart valvular lesions. I am working on trimming and sectioning methods to get uniform and reproducible morphology of heart valves in paraffin sections. It seems that available literature dealing with this is limited. Requesting your

[Histonet] Tissue processing protocol for Hamster tissues

Dear Histonetters, We were asked to process Hamster tissue(Large intestine) for one of our investigators. I would like to know whether any of our users process hamster tissues in their labs, if so kindly provide me a protocol for the same. Our tissue processor is Sakura VIP6. Thanks for all

[Histonet] Your experience with microm HMS 740 auto stainer

Dear Histonetters, We would like to procure one tissue auto stainer for our histology lab(mainly HE staining) In this respect, I request your experience about Microm HMS 740 automatic stainer. Please share your comments regarding the staining reproducibility,ease of handling and their claim