Re: [Histonet] Microtome calibration

2008-11-01 Thread anhtx
useless exercise. We do have a quality check before the slides reach Pathologist for review. But these guys are not convinced. Thanks once again Regards Girish - Forwarded by ANHTX DR/DR/DRL/IN on 01/11/2008 02:00 PM

[Histonet] Microtome calibration

2008-10-30 Thread anhtx
Dear all Is anyone practising microtome calibration for thickness of sections cut? Our GLP auditors are frequently asking for it. They also suggest that automatic tissue processor time in each reagent should be calibrated. any comments Regards Dr Girish India Disclaimer This message contai

[Histonet] mitotic figures-staining protocol

2008-10-26 Thread anhtx
Thanks Carl for your valuable suggestions on anti- phospho-Histone H3 antibody. But i will be glad if someone can share the staining protocol for Toluidine blue and Crystal violet to demonstrate mitotic figures. Regards Girish Disclaimer This message contains legally privileged and/or confid

[Histonet] mitotic figures staining

2008-10-24 Thread anhtx
Hello everyone This is Girish working as toxicologic pathologist for a drug discovery firm based in India. We are in the process of evaluating mitotic indices/figures in the liver sections of rat. Can anyone help regarding what kind of simple histotechniques are useful for this ? When we went