Re: [Histonet] linear stainer hematoxylin staining issues

How do I unsubscribe from Histonet?? Thank you, Arjun On Wed, Mar 27, 2013 at 1:46 PM, Kienitz, Kari kkien...@orclinic.comwrote: If your acetic acid solution is made in-houseI would suggest remaking it, just in case it was accidently made stronger. Kari Kienitz HT, (ASCP) Histology

Re: [Histonet] How to fix frozen section?

i use 1:1 Acetone : alcohol. On Nov 6, 2012, at 2:41 PM, Amy Lee wrote: Hello, I will receive some frozen tissue sections that are not fixed. I rarely do IHC on frozen sections. I searched some articles about fixation. Some use 50/50 of acetone/alcohol and some use only alcohol. Could

[Histonet] Fwd: MIXED fiber type identification by Immunohistochemistry

to hear people's advices this time. Thanks Best, Arjun Begin forwarded message: From: arjun ruhella ruhella.ar...@gmail.com Date: October 2, 2012 8:34:59 AM EDT To: histonet@lists.utsouthwestern.edu Subject: MIXED fiber type identification by Immunohistochemistry Greetings to all

Re: [Histonet] Mixed fiber type identification

Thanks Michael! I really appreciate your suggestions. That being said, i really cannot go for mATPase or PAS stain, I need to do IHC. I hope to hear from other too on this issue. Thanks AR On Oct 4, 2012, at 1:20 PM, mtitf...@aol.com wrote: Arjun Ruhella asked about fiber typing of animal

[Histonet] MIXED fiber type identification by Immunohistochemistry

Greetings to all, In immunohistochemistry, I am encountering confusion in designating muscle fibers (based on staining color intensity) to a particular fiber type. This problem is particularly bothersome when i encounter the fibers stained with 2 different colors, one color has good

[Histonet] How can i post a question in histonet?

Please let me know! Thank you Best, AR ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

Re: [Histonet] How can i post a question in histonet?

...@lists.utsouthwestern.edu] On Behalf Of arjun ruhella Sent: Thursday, September 20, 2012 1:49 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] How can i post a question in histonet? Please let me know! Thank you Best, AR ___ Histonet

[Histonet] Is specimen too cold for cryosectioning?

Dear all, I have heard a little trick in cryosectioning where, if the specimen is too cold, one put thumb on the specimen and then cut immediately. I have a question related to this. Q1 = My cryostat temperature cannot be increased above -20C (a problem needs to be fixed). I usually tend to