[Histonet] Masson Trichome Staining: Can I use Fast Green?

Hi Histonet, I am currently using the protocol from Stainsfile for staining heart tissue: http://stainsfile.info/StainsFile/stain/conektv/masson.htm My lab had Fast Green readily available so I substituted it for Light Green in Solution C. The slides have not been coming out well. I failed to acc

[Histonet] Refixing with Bouin's for Masson's Trichrome

Hi Histonet, I am currently refixing my slides in Bouin's solution. Should I be refixing the entire tissue sample? What I mean is, should I deparrafinize my heart samples, refix in Bouin's, and reinfiltrate them before slicing with the microtome? Or is it more effective to just refix the slides wi

[Histonet] Removing Yellow color from slides refixed in Bouin's solution

Hi Histonet, After placing slides in Bouin's for 1 hour at 56 degrees, I am finding it very difficult to remove the yellow coloring. Rinses with water are not doing the trick. Does anyone have some advice on how to bring the slides back to a clear color so that I may proceed with the Masson Trichr

[Histonet] Masson's Trichrome, Weigert Hematoxylin Equivalent

Hi Histonet, I am performing the trichrome stain on cardiac tissue. The procedure calls for Weigert's iron hematoxylin stain or equivalent. Would Harris hematoxylin work as well. It's what I have readily available at the lab. The instructions for preparing the Weigert stain involves mixing fresh

[Histonet] Masson Trichrome, Alligator Heart Tissue, colors turning out purple

To Histonet: This is my first time mailing the histonet community. Coincidentally, this is my first venture into histology. My lab is conducting research on alligator heart tissue. At the moment, I am attempting to stain my slides using Masson Trichrome to differentiate muscle from connective tiss