[Histonet] looking for a good anti-mouse CD3 for formalin fixed sections

Hi, Could anyone suggest a good anti-mouse CD3? I am looking at staining aortic root frozen sections fixed with formalin. Thanks! Delphine Delphine Eberlé PhD UCSF Department of Vascular Surgery VA Medical Center - NCIRE Building 2 - room 410 4150 Clement Street San Francisco, CA 94121,

[Histonet] Autofluorescence problem on atheroma - TUNEL staining

Hi, Autofluorescence in atheroma is a well known problem for immunofluorescence staining. I would love to hear input about how to reduce it to a minimum (if possible!). So far, we are trying to detect apoptotic cells in atheroma by TUNEL staining (Roche, TMRred) and we found the

[Histonet] Stains for Macrophages for Laser Capture Microdissection purpose

Hi, I have another question following Macrophage staining. I am setting up a Laser Capture Microdissection protocol to dissect macrophages from atherosclerosis lesions (non fixed frozen sections) and extract RNA for gene expression purpose. I am looking at a quick staining (less than