[Histonet] IHC protocol for 5 year old Pap smear slides

Hi, everyone I want to do pan-keratin IHC for some five year old pap smear slides. After I remove the coverslip then I used following protocol for destaining (xylene 5 min, two time change; 3% acetic alcohol (95%) overnight; 70% Etoh for 5 min) and then I put it in PBS, blocking, Prmary Ab, Second

[Histonet] antigen retrieval for 5 year pap smear slides

minutes and then SDS (0.5% or 0.25%) treatment for 10 minutes. But all method did not work. Any replies will be very appreciated. -- Hao ZHANG, Ph.D Scientist ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu