Hi Angela,
We came across similar situation during our GLP inspection. As Morken has
mentioned the validation would certainly stand by us, more than the expiry
dates in the bottle. We give 3 months expiry for any reagents prepared in our
lab and usually it get consumed by that time. I would sug
Dear all,
We stained decalcified paraffin sections of rodent femur by alizarin red S
using the following protocol -
a. deparrafinised in xylene
b. 100%ethaonol->95% ethaonol-->70% ethaonol-->distillled water.
c. dipped in 2%alizarin red S, dissolved in distilled water (pH adjusted with
12% NH4O
Hi Gayle,
I do get your view on making a judgement. We do our own issues at times, in
microtomy/staining.
Nicely elaborated.I would like to go through the publication to have a better
understanding.
Pl. do share to my email- ragh...@orchidpharma.com.
Thanks,
raghul
-Original Message-
F
Dear all,
In the weight loss/gain method how would we fix the optimal endpoint to stop
the decal process based on weight gain? Will weighing omit the other ways of
judgement such as needle prick, physical examination. In our tox experiments we
deal with lot of femurs in one stretch and weighin
Hello,
Have any one succeeded in using anti-CD3 mouse antibody meant for frozen
section in ffpe. I am in a situation to try and have planned antigen retreiveal
to improve the situation. Any suggestion would be helpful
Thanks,
raghul
-Original Message-
From: histonet-boun...@lists.utsou
Dear histonetters,
I have pasted two images img001 and img002 dt. 7feb2011 on staining. The images
are of mouse kidney 4micron paraffin embedded formalin fixed sections where
the hematoxylin stained areas are pale and not uniform. Is it because of over
heating or sections getting dried during
