[Histonet] RE: Help! Losing sections from Superfrost Plus Slides

Hi Angelina, We were having the same problem with frozen mouse aortas. I was cutting at 5ums onto superfrost slides and was losing about half of my sections. I ended up swapping to superfrost plus ultra, plus I bake the slides in the histology oven at 60 degress celcius for 10 minutes and

[Histonet] non-specific fluorescence in artery

anti-mouse), 1:50 diln - 1h TBS with tween - 3x 3 changes secondary Ab (Alexa Fluor 594, goat anti-rabbit), 1:800 - 1h TBS with tween - 3x 3 changes DAPI Thanks, Sarah Sarah Tarran Postdoctoral Fellow Vascular Biology Research Centre, Department of Surgery Westmead Hospital, Westmead, NSW, 2145

[Histonet] Re: IHC chromogen loss, HELP!

directly to meghan.tuc...@ars.usda.gov would be appreciated Sarah Tarran Postdoctoral Fellow Vascular Biology Research Centre, Department of Surgery Westmead Hospital, Westmead, NSW, 2145 02 98455775 sarah_tar...@wmi.usyd.edu.au ___ Histonet mailing

[Histonet] Re: need help with Elastase staining

mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 63, Issue 12 Sarah Tarran Postdoctoral Fellow Vascular Biology Research Centre, Department of Surgery Westmead Hospital