RE: [Histonet] Thermo Shandon Histobathe

Liquid nitrogen in a carboy? Why do you need a fancy machine just to snap freeze? Sounds like Thermo trying to rip people off?? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Messa

RE: [Histonet] Uni-trieve, antigen retrieval for immunofluorescence

I actually attended a class they put on a few months ago where they used Unitrieve. It works really well. It doesn't seem to produce any kind of background and yes, it is used at much lower temps. However the retrieval process takes a little longer, but...you can put it in an even lower temp. wa

[Histonet] IHC ?

Hello all, So I just realized I am out of my block after I have done HIER. Can I leave the slides in buffer in the fridge or something overnight. The block will be here at 10am tomorrow morning... She the slides be ok or do I need to re-cut all of them and start over (please tell me this is not

histonet@lists.utsouthwestern.edu

This happened to me several months ago. Our lab always has high humidity. If you are going to continue to use clear rite with humidity in the air, I would change it everyday. Also, change out your 100% because it's inevitably getting water in it too. I just switched my whole lab back to xyle

RE: [Histonet] immunohistochemistry: manual staining

I tried ordering this system a few months ago, and it was discontinued? Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [m

RE: [Histonet] Facebook

You can search it in groups too...it has and H&E heartwell actually it's some kind of lumen in the shape of a heart. I don't remember the specific organ =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900

RE: [Histonet] I'm so sad for AFIP to close

Wow...that is sad news. Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message- From: histonet-boun...@lists.utsouthwestern.edu [mailto:histonet-boun...@lists.utsouthwestern.edu]

RE: [Histonet] gout processing procedure

DO NOT let the sample come in contact with water!!! Let them fix in 100% alcohol. You will also need to set up a run cycle on the processor that does not have any water either (ie, no formalin or alcohol gradients). Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Wood

RE: [Histonet] peggy wenk comments on HT/HTL practical

I have been required to cut slides at every job interview I have ever been to. I think this is good practice to follow because if you know what you are doing you should be fine and have no worries. If you are a book tech and have no practical experience then you should not be hired over someone w

RE: [Histonet] knife used for cleaning paraffin off the blocks

The melters are pretty cool, but they have a pretty cool price tag too! If you are on a budget, this is not practical. I use a scalpel. Just make sure you dull the edge a bit before you use it so you don't chop your fingers off =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therape

[Histonet] hmm

Has anyone ever heard of the company, Stem cells? I have been trying to contact them all morning to order an antibody, but no one ever answers the phone. Little nervous. Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 7

[Histonet] Oops

That was backwards =) Don't stain mouse cells...stain human ones Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 ___ Histonet mailing list Hi

[Histonet] Mouse vs. Human cells

Hello Histo-hotties! Question: We are working with xenograft tumors in mice. The tumor cells are human in origin. I am trying to come up with some kind of stain that will stain all the mouse cells and none of the human cells. Just to be able to determine if any of the human tumor cells are in a

RE: [Histonet] which conferences?

NSH!!! Great information...great people...great locations...and great nightly parties =) It's September 16 this year. http://s3.goeshow.com/nsh/annual/2011/index.cfm Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)

RE: [Histonet] Formalin down the drain??

Been reading all these responses...so funny how different places do different things. An old job I worked at poured gallons and gallons down the drain on a daily basis. I do think there was some kind of filter system in place, but all the same...how do you filter out all the little things?? I

[Histonet] Autoclave water

Hello all, We are having an issue with water (Millipore filtered and tap is the same problem). We put the water into glass pyrex jars (the kind with the orange lid) and autoclave. After they are done there are tiny little naked eye visible particles of stuff floating in the water. It shimmers a

RE: [Histonet] What is a great manual mirotome

I am a manual tome junky! I like the Thermo Finesse (I think it's 325). These are the old microms...they work like a charm =) Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -Original Message-

[Histonet] Test

So I got married and my name changed (meaning so did my email) tried sending out a broad email, but it didn't work. Just making sure I'm updated now =) Happy Monday all!! Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas