I actually attended a class they put on a few months ago where they used Unitrieve. It works really well. It doesn't seem to produce any kind of background and yes, it is used at much lower temps. However the retrieval process takes a little longer, but...you can put it in an even lower temp. water bath overnight, come in to work in the morning, and your slides are reading to go. Contact Zara over there and she might be able to hook you up with a sample. Good Luck!!
Sarah Goebel-Dysart, BA, HT(ASCP) Histotechnologist Mirna Therapeutics 2150 Woodward Street Suite 100 Austin, Texas 78744 (512)901-0900 ext. 6912 -----Original Message----- From: [email protected] [mailto:[email protected]] On Behalf Of Andrea Marion Sent: Tuesday, September 20, 2011 11:59 AM To: [email protected] Subject: [Histonet] Uni-trieve, antigen retrieval for immunofluorescence Hello all, Has anyone used the reagent 'Uni-trieve' from Innovex? It is purported to be a universal antigen retrieval solution that can be used at lower temperatures (65-70 C for cytoplasmic antigens, and 75-80 C for nuclear antigens): http://innvx.com/unitrievepage.html The company claims that the reagent is a universal retrieval solution for all antibodies and tissues (which is silly of course - how could they know?). Does anyone have any experience with the product? I am interested because I see that increased heat during antigen retrieval causes greater tissue autofluorescence during immunofluorescence stainings. My current protocol is to use 20 minutes at 90-95 C on a hot plate using sodium citrate buffer. Does anyone else either Uni-trieve or a different reagent/protocol for immunofluorescence stainings? Thanks, Andrea Andrea Marion Graduate Student University of Illinois at Chicago _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet _______________________________________________ Histonet mailing list [email protected] http://lists.utsouthwestern.edu/mailman/listinfo/histonet
