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I am looking to find a control block for EBV. I have blocks I can trade
(melanoma or H. pylori). Please contact me if anyone is interested. Thank you
Vicky Hammel HTL, ASCP
Pathology Technical Consultant
vham...@primecare.orgmailto:vham...@primecare.org
St. Alexius Medical Center
Histology
Please unsubscribe.
thanks
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Greetings, friend!
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Good Morning Histonet,
Ok, We are just starting up with MSI testing in our facility and a
question has popped up about diagnosis of these slides... Are you
looking for a COMPLETE LOSS of this protein in the tumor, or just a loss
of some expression.? So if you have a normal piece of tissue
Complete loss. You can see patchy immunoreactivity in tumors with these
markers.
Richard
Richard W. Cartun, MS, PhD
Director, Histology Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT 06102
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Hey histo gurus:
Just a little twist for you. We are planning on doing GMS and PASD on frozen
skin sections. Is there any change in staining protocol for paraffin vs frozen.
We plan on fixing in ETOH. I have a co-worker saying that we don't need to use
diastase on the PASD because there are
of the heat but,
other than that, there is no reason.
René J.
--- On Tue, 2/14/12, Ingles Claire cing...@uwhealth.org wrote:
From: Ingles Claire cing...@uwhealth.org
Subject: [Histonet] (no subject)
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, February 14, 2012, 2:22 PM
Hey histo gurus
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ingles Claire
Sent: Wednesday, 15 February 2012 6:22 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
Hey histo gurus:
Just a little
Claudia N. Segovia
Neurohistologist
NeuroScience Associates
10915 Lake Ridge Drive
Knoxville, TN 37934
865-675-2245
csego...@nsalabs.com
STATEMENT OF CONFIDENTIALITY:
The information contained in this electronic message and any attachments are
intended for the exclusive use of the
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Hello All,
I have completed my 2011 statistical analysis of our ER/PR results,
comparing them to 'published benchmarks' as per CAP/ASCO guidelines.
Has anyone also performed this for their Her2 testing? What benchmarks
did you use? Can you site your source? We are currently just running
Her2 FISH.
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Which is the best way to process small biopsies and lymph nodes manually?
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Good Morning HistoWorld, I am looking for a lab that runs Hepatitis C
antibody by Immunohistochemistry. We have autopsy tissue we would like
run, so research use only or ASR is fine. Any leads would be greatly
appreciated. The only lab I found was in Pakistan and I was hoping to
keep it a
] On Behalf Of Freeman, Carol
[carol.free...@utoledo.edu]
Sent: Thursday, December 15, 2011 10:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
Good Morning HistoWorld, I am looking for a lab that runs Hepatitis C
antibody by Immunohistochemistry. We have autopsy
Ok
Sent via BlackBerry by ATT
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Hello All,
We are having a issue with our intercom system in the OR so the pathologist
are having issues communicating frozen section diagnosis with the surgeon.
Does anyone in Histoland use a written form to send frozen section
diagnosis? Per CAP if it is verbal the pathologist must speak
...@shorememorial.org
Sent: Wednesday, November 30, 2011 10:20 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
Hello All,
We are having a issue with our intercom system in the OR so the pathologist are
having issues communicating frozen section diagnosis with the surgeon.
Does
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We are starting MSI testing in our lab, I am just curious if there is
any labs out there doing this on colon biopsy cases. If so we would
love some feedback. I would like to know if this is common practice
somewhere.
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Because your boss said to leave it at 84 degrees?
Emily
The whole point of this country is if you want to eat garbage, balloon up
to 600 pounds and die of a heart attack at 43, you can! You are free to do
so. To me, that’s beautiful.
--Ron Swanson
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Hello
I am currently studying to become a histotech. I wanted to know why should
the paraffin in the microwave processor be kept at 84 degrees.
Thanks
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Hi all
Is anyone of you familiar with the staining of CD4 cells in minipigs in
formalin fixed and paraffin embedded tissues?
Joost
TNO.NLhttp://www.tno.nl/
Joost Bruijntjes
T +31 88 866 17 38
F +31 30 694 49 86
E joost.bruijnt...@tno.triskelion.nlmailto:joost.bruijnt...@tno.triskelion.nl
Histonet mailing list submissions
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From: horten_...@hotmail.com
To: histonet@lists.utsouthwestern.edu
Date: Thu, 20 Oct 2011 09:29:21 +0200
Subject: [Histonet] (no subject)
Histonet mailing list submissions
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Miha Tesar
Sent: Saturday, October 15, 2011 1:47 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
Hi!
I am working in Institute of Phatology Slovenia EU. Recently I have problems
with muscle biopsy. There are a lots
, 2011 4:47 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
Hi!
I am working in Institute of Phatology Slovenia EU. Recently I have problems
with muscle biopsy. There are a lots of artefact that I can not explain, like
little holes in the tissue after the tissue is being
...@lists.utsouthwestern.edu] On Behalf Of Garrison, Becky
Sent: Monday, October 17, 2011 1:41 PM
To: 'Miha Tesar'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] (no subject)
I am forwarding this response from a fellow worker, D Kaylor
(The techs here have consistently good results when freezing
@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
Hi!
I am working in Institute of Phatology Slovenia EU. Recently I have problems
with muscle biopsy. There are a lots of artefact that I can not explain, like
little holes in the tissue after the tissue is being frozen. We are using
Hi!
I am working in Institute of Phatology Slovenia EU. Recently I have problems
with muscle biopsy. There are a lots of artefact that I can not explain, like
little holes in the tissue after the tissue is being frozen. We are using the
isopentan and the liquid nitrogen for the freezing. Can
Hi everybody!
I'm interested in making a Prussian Blue (Potassium ferrocyanide) staining to
highlight micro vascular infarctus in human brains.
In the protocol I have, it's written that a nitric acid pre-treatment is
necessary.
I would like to know:
1) What nitric acid is used for in
it is no longer an automatic response
from all involved that it is Histology's fault. Hope you are enjoying your
new adventures in retirement. See you next year in Canada!
From: mamaw...@hotmail.com
To: histonet@lists.utsouthwestern.edu
Date: Wed, 5 Oct 2011 14:11:25 -1000
Subject: [Histonet
@lists.utsouthwestern.edu
Date: Wed, 5 Oct 2011 14:11:25 -1000
Subject: [Histonet] (no subject)
Hello everyone,After being home from the NSH for a few weeks I have been
pondering an issue that I think bears discussion on the histonet.There have
been several papers published regarding floaters
I too spoke to one vendor who claimed that the floaters are not an issue and
that their bottles drew below the level of the floating floaters. I didn't
feel insulted, but did feel that this salesperson obviously had no actual lab
experience or they never would have made such a ridiculous claim.
Very good question Cindi.Jan
Date: Thu, 6 Oct 2011 09:26:18 -0400
From: robin...@mercyhealth.com
To: mamaw...@hotmail.com; histonet@lists.utsouthwestern.edu;
christiego...@msn.com
Subject: RE: [Histonet] (no subject)
It is a good thing this vendor does not work in a histo lab because
Hello everyone,After being home from the NSH for a few weeks I have been
pondering an issue that I think bears discussion on the histonet.There have
been several papers published regarding floaters and the amount determined to
come from traditional staining buckets. There was also a poster
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Sylvia Hewell, HT (ASCP)
Histology Supervisor
ProPath
Lewisviile Medical Center
500 W. Main St.
972-420-1552 or 972-420-1887
To learn more about ProPath, please visit http://www.ProPath.com
http://www.propath.com/
sylvia.hew...@propath.com
The information contained in this message
Hi all
I have to prepare frozen slides of zebra fish, but it is not permitted to use
OCT because of the presence of different polymers in the OCT compound. Is
anyone of you familiar with the preparation of frozen slides without using OCT?
Thanks
Joost Bruijntjes
Zeist
Holland
the coolant aerosol make sure that the drop is completely
frozen. Proceed to cut.
René J.
--- On Tue, 9/6/11, Bruijntjes, J.P. (Joost)
joost.bruijnt...@tno.triskelion.nl wrote:
From: Bruijntjes, J.P. (Joost) joost.bruijnt...@tno.triskelion.nl
Subject: [Histonet] (no subject)
To: Histonet
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
Hi all
I have to prepare frozen slides of zebra fish, but it is not permitted to use
OCT because of the presence of different polymers in the OCT compound. Is
anyone of you familiar with the preparation of frozen slides
Dear histonetters
I#39;m working on a surface marker, uPAR, on lymph node tissue. For setting up
the IHC-p procedure, I#39;ve chosen the ductal carcinoma sample as positive
control. I use the citrate buffer ph=6 as retrieval solution and TBS ph=7.6 for
wash steps. different Ag retrieval
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How many labs have data entry people assigning surgical case numbers in their
processing departments? Basically how many histology departments have AP
surgicals processed like clinical specimens?
Cheryl A. Miller HT(ASCP)cm
Histology/Cytology Prep Supervisor
Physicians Laboratory Services
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Ruthie,
In order to unsubscribe from the list, you must click on the link at the bottom
of the email, scroll to the bottom of the webpage and enter your email
requesting to be unsubscribed. You have the power to unsubscribe yourself!
Good Luck!
Michelle
Sent from my iPhone
On Jul 26, 2011,
Good Morning All,
We do the non-gyn cytology cases in the histology lab and usually I will stain
the body fluids separate. Now that our workload has increased staining these
specimens separately takes up a lot of time. I was wandering what other labs
were doing to prevent cross-contamination
Please unsubscribe me
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Does anyone have a good protocol for Giemsa? I really need it
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For tissues or smears? I can send you mine for tissues.
René J.
--- On Tue, 6/28/11, Heather Cooper hctrup...@att.net wrote:
From: Heather Cooper hctrup...@att.net
Subject: [Histonet] (no subject)
To: histonet@lists.utsouthwestern.edu
Date: Tuesday, June 28, 2011, 3:52 PM
Does anyone have
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Dear Histonets,
How is it possible to validate a antibody on the Ventana Ultra or XT, when the
antibodys afre prediluted or made into a prep-kit?
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...@lists.utsouthwestern.edu] On Behalf Of Dorothy
Glass
Sent: Wednesday, June 15, 2011 9:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
Dear Histonets,
How is it possible to validate a antibody on the Ventana Ultra or XT,
when the
antibodys afre prediluted or made into a prep
Dorothy,
Is your question related to the fact that a protocol will have the currently
active lot number and you want to use a different lot number? Or is this a
brand new antibody that has not been in use before and therefore has not ever
been validated?
Michelle
On Jun 15, 2011, at 11:41
J.
From: Dorothy Glass techman...@yahoo.com
To: histonet@lists.utsouthwestern.edu
Sent: Wednesday, June 15, 2011 11:41 AM
Subject: [Histonet] (no subject)
Dear Histonets,
How is it possible to validate a antibody on the Ventana Ultra or XT, when the
antibodys afre prediluted or made into a prep
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I have a new carbon filter for a Leica Coverslipper Model CV5000 that I ordered
by mistake. It is from Mercedes Medical, part #MER440122. It is free to whoever
will pay the shipping to your facility.
Thanks,
Robin Taylor HT
Butler Cty Medical Center
3145 Hamilton ason Rd.
Hamilton, OH 45011
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...Ive got something for you!
http://www.sweep.co.il/page.php?jcyhotmailID=07o5
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The C-Kit CD117 antibody from Ventana now has the following FDA approval:
C-Kit has PMA FDA approval along with a companion diagnostic approval to
utilize Gleevac therapy when positive for GIST tumors.
Since the cost is now very high for the antibody and since it is used for
predictive
an
anatomical alteration that accompanies it but cannot measure. Have your
pathologists seriously consider this issue?
René J.
From: Dorothy Glass techman...@yahoo.com
To: Histonet@lists.utsouthwestern.edu
Sent: Monday, May 9, 2011 8:50 PM
Subject: Re: [Histonet] (no subject)
What is an IHC stain
techman...@yahoo.com
To: Histonet@lists.utsouthwestern.edu
Sent: Monday, May 9, 2011 8:50 PM
Subject: Re: [Histonet] (no subject)
What is an IHC stain for gastric reflux. Pathologists are wanting to start
using
the procedure. Can anyone help?
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What is an IHC stain for gastric reflux. Pathologists are wanting to start
using
the procedure. Can anyone help?
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First of all make sure your designated outlet has its own power
surge within it. Talk with your building electrician. They should be able to
accommodate you.
If you still feel you need extra protection you maybe able to just install a
power surge protector.
THANK YOU,
PATTI RUBEN-NELSON
us immediately. Thank you.
-Original Message-
From: histonet-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of SHANE NELSON
Sent: Thursday, April 21, 2011 12:37 PM
To: Histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)
Hi
Hi from Southern Cali,
We neutralize our formalin with a product called Neutralex. It comes in powder
form and after 30 mins we can dump it down the drain. We also keep
records for QC purposes. Product is purchased from a company called American
MasterTech. Hope this gives you a starting place.
We are currently in need of some ergonomic chairs for our embedding and
microtomy stations. Does anyone have any
informatio to share in reguard to chairs?
__
The contents of this message may contain private, protected and/or
Yes - make sure they're long enough in the SEAT! Mine roll around the
lab like little 5-wheeled rockets, but cut off the circulation to my
thighs (is that something I can say on television?). And no arms on the
chairs!
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Can anyone tell me how and who they use to do radiation level checks in their
laboratories? I was hoping to find something similar to the formalin
monitoring but I am not having much luck.
Dawn Gullifer BS, HT (ASCP)
Laboratory Manager
OSU Histology Lab, LLC
614-293-0358 office
614-293-0345
-boun...@lists.utsouthwestern.edu
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of
Gullifer, Dawn
Sent: Tuesday, March 29, 2011 12:24 PM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] (no subject)
Can anyone tell me how and who they use to do radiation level checks
St. Anthony Hospital currently has an excellent opportunity for an experienced
Histologic Technician. This position requires Certification as an HT or HLT –
or – experience acceptable to the Laboratory Director. Two years of previous
histology experience required with IHC and/or grossing
Dear,
Can you please unsubscribe me from the Histonet list?
It was a pleasure to read all the good feedback.
Regards
An Eerdekens
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Dear also
The only person who can unsubscribe you is yourself. Go to the HistoNet
website and take care yourself of your request.
René J.
--- On Fri, 3/18/11, An Eerdekens an.eerdek...@med.kuleuven.be wrote:
From: An Eerdekens an.eerdek...@med.kuleuven.be
Subject: [Histonet] (no subject
Are there any Histo Techs in the Stockton, CA area looking for a full time
position?
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