25-2-2010 16:42
Aan: histonet@lists.utsouthwestern.edu; histonet.nos...@vneubert.com
Onderwerp: Re: [Histonet] [ SOLVED ][ Histonet ] Strange circles in IHC slides
To me it seems that the sections after being picked from the water bath were
not completely drained and the dewaxing process
Hello,
I'm glad that you already solved the problem your way.
I didn't read your first post, but we had exactly the same problem (and
we're also using cover plates). This artifact is very likely caused by tiny
air bubbles which are trapped under the cover plate. The crucial step is
when you drip
Hello Histonet,
it has been a while (~10 months) since I posted a problem about uneven
immuno-staining with specimen showing unstained circles after manual staining
with HRP-polymere/DAB method; complete mail see below, response mails see
Histonet archive (via website).
Links to pictures I
but the detergent will mix with the water
and will better remove the paraffin.René J.
--- On Thu, 2/25/10, histonet.nos...@vneubert.com
histonet.nos...@vneubert.com wrote:
From: histonet.nos...@vneubert.com histonet.nos...@vneubert.com
Subject:[Histonet] [ SOLVED ][ Histonet ] Strange
