To dry them overnight before fixation is not a bit too risky? I mean,
wouldn´t the tissue degradate? I normaly fixed the section as soon as I
get it into the slide.
Just a thought. I am no expert in cryostate, just an occasional user.
Julio
"Lewis, Patrick" escribió:
Hi Everyone,
I am sti
Hi Everyone,
I am still having issues with my IHCs with Acetone fixation.
If I fix in 100% Acetone, I get IHC staining, but my tissues are 50-90%
destroyed.
If I fix in 4% paraformaldehyde, or 10% NBF or (95% Etoh and/or Methanol with
Acetone) I lose the epitopes I either get no staining or
Have questions about using acetone at the grossing counter. Does
anyone have problems with it being carried over into the formalin on
the processor? We rinse, open cassette to let it dry completely and
even change the cassette. I've had issues with my processor and want
to make sure
