Re: [Histonet] BRAF testing

972-1596 (Office) > (860) 545-2204 (Fax) > richard.car...@hhchealth.org > > -Original Message- > From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] > Sent: Friday, March 27, 2020 8:07 AM > To: Histo List > Subject: [Histonet] BRAF testing > > T

Re: [Histonet] BRAF testing

Hospital 80 Seymour Street Hartford, CT 06102 (860) 972-1596 (Office) (860) 545-2204 (Fax) richard.car...@hhchealth.org -Original Message- From: Charles Riley via Histonet [mailto:histonet@lists.utsouthwestern.edu] Sent: Friday, March 27, 2020 8:07 AM To: Histo List Subject: [Histonet] BRA

[Histonet] BRAF testing

Can anyone send me a block that can be used to validate BRAF V600E antibody. I have tried several cases that should work but our pathologists don't think it is working correctly. I just need a small core sample that is known to be positive. Any help would greatly be appreciated -- Charles Rile

[Histonet] BRAF KRAS NRAS by PCR

Does anyone do these tests using PCR on FFPE blocks? If so what platform do you use/recommend? -- Charles Riley BS HT, HTL(ASCP)CM Histopathology Coordinator/ Mohs ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthweste

[Histonet] Braf protocol on Bond-III

1:100 with ER-2 for 30 mins. Clone is the V600E from Spring BioScience Looks great for uis and we are performing well on EQA surveys. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* __

[Histonet] braf (v6001e)

Does anyone use this antibody on the Leica Bond systems? I have used several different vendors to validate this antibody but the Docs say all of them are over staining or not working correctly (I've gone down to 1:5000 on the dilution on all of them). If anyone has any suggestions on protocols o

Re: [Histonet] BRAF

Hi, Thank you Greg. Samira From: Greg Dobbin [mailto:greg.dob...@gmail.com] Sent: Tuesday, July 24, 2018 2:17 PM To: Alminawi, Samira; histonet@lists.utsouthwestern.edu Subject: RE: BRAF Here in Charlottetown we are using V600E (VE-1) clone (Monoclonal) from Spring Diagnostics. We run it on the

Re: [Histonet] BRAF

Yes, it works. Very well actually. And we have done well on both a cIQc and a CAP survey. Greg On Tue, Jul 24, 2018 at 3:20 PM Alminawi, Samira < samira.almin...@sunnybrook.ca> wrote: > Hi Greg, > > How is the quality of the staining? Is it working for melanoma? > > Samira > > > > *From:* Greg Do

Re: [Histonet] BRAF

Hi Greg, How is the quality of the staining? Is it working for melanoma? Samira From: Greg Dobbin [mailto:greg.dob...@gmail.com] Sent: Tuesday, July 24, 2018 2:17 PM To: Alminawi, Samira; histonet@lists.utsouthwestern.edu Subject: RE: BRAF Here in Charlottetown we are using V600E (VE-1) clone (Mo

Re: [Histonet] BRAF

Here in Charlottetown we are using V600E (VE-1) clone (Monoclonal) from Spring Diagnostics. We run it on the Bond platforms at 1:100 with ER-2 (high pH) for 30 mins. Greg -- *Greg Dobbin* 1205 Pleasant Grove Rd RR#2 York, PE C0A 1P0 *Everything in moderation...even moderation itself**!* __

Re: [Histonet] BRAF

I am interested in the same question. If responding please remember to use the reply all option so everyone can hear the answers. On Tue, Jul 24, 2018 at 9:13 AM, Alminawi, Samira via Histonet < histonet@lists.utsouthwestern.edu> wrote: > Hi, > Anybody using BRAF antibody for melanoma, what is th

[Histonet] BRAF

Hi, Anybody using BRAF antibody for melanoma, what is the recommended clone and protocol? samira This e-mail is intended only for the named recipient(s) and may contain confidential, personal and/or health information (information which may be subject to legal restrictions on use, retention a

Re: [Histonet] BRAF V600E

Sent: Tuesday, December 13, 2016 2:50 PM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BRAF V600E Good Afternoon Histonet! It's been a few years since I posted this question initially; thought I'd give it a try again. Is anyone successfully utilizing Roche's BRAF V60

[Histonet] BRAF V600E

Good Afternoon Histonet! It's been a few years since I posted this question initially; thought I'd give it a try again. Is anyone successfully utilizing Roche's BRAF V600e (VE1) (or Spring Bioscience's) antibody on a Leica Bond Max? If you are, I'd really appreciate hearing how you did it. T

[Histonet] BRAF by immunohistochemistry

Does anyone know of a place we can send a case to for BRAF immunohistochemistry? Hazel Horn Supervisor of Histology/Autopsy/Transcription Anatomic Pathology Arkansas Children's Hospital 1 Children's Way | Slot 820| Little Rock, AR 72202 501.364.4240 direct | 501.364.1241 fax hor...@archildrens.org

[Histonet] BRAF (V600E) on BondMax

Hey Histonetters, Has anyone successfully worked up Spring Bioscience's BRAF (v600e) on the Leica Bond? We've experimented with different pretreatment options and serially diluted the heck out this antibody (up to 1:6,400) and we're still getting background and unexpected staining. Our patho

[Histonet] BRAF V600E

Is anyone running NewEast BRAF V600E (cat 26039) on the Dako Link48 with Envision FLEX? I am wondering what your dilution and retrieval are. I am having trouble with this antibody. Kathryn Stoll Department of Pathology Medical College of Wisconsin 9200 W. Wisconsin Ave Room 1176 Ph: 414-805-1

[Histonet] BRAF V600 D & V600 K

Hello, Histoland, I would appreciate any help you can offer on these antibodies. Does anyone have experience with the BRAF V600 D and V600 K? Are you running in colorectal specimen or in melanocytic (skin) tumors? Many Thanks, Susan Foreman, HT (ASCP) KDL Pathology 315 Erin Drive

RE: [Histonet] BRAF IHC

OH, Wrong Pat! :) -Original Message- From: Susan Foreman [mailto:sfore...@labpath.com] Sent: Wednesday, July 10, 2013 11:05 AM To: Bell, Pat; histonet@lists.utsouthwestern.edu Subject: RE: [Histonet] BRAF IHC I was attempting to respond to this older post: Thanks Pat Zeitlow pzeitlow

RE: [Histonet] BRAF IHC

I was attempting to respond to this older post: Thanks Pat Zeitlow pzeitlow <@t> bbpllab.com Fri May 3 13:52:49 CDT 2013 Previous message: [Histonet] BRAF IHC Next message: [Histonet] BrDU Messages sorted by: [ date ] [ thread ] [ subject ] [ author ] Also using Spring Bios

RE: [Histonet] BRAF IHC

25 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BRAF IHC Pat, I was wondering how your BRAF IHC staining is going? We have the same antibody working using Ventana Benchmark Ultra. We do use amp kit. With you post, did anyone respond that was using other clones? V600K

[Histonet] BRAF IHC

Pat, I was wondering how your BRAF IHC staining is going? We have the same antibody working using Ventana Benchmark Ultra. We do use amp kit. With you post, did anyone respond that was using other clones? V600K or D? Many Thanks, Susan Foreman (865)584-1933

RE: [Histonet] BRAF

...@lists.utsouthwestern.edu] On Behalf Of Mary Ann Deathridge Sent: Wednesday, May 29, 2013 11:48 AM To: histonet@lists.utsouthwestern.edu Subject: [Histonet] BRAF Hi Hisonetters Looking for information on BRAF-(V600E) IHC. Any information (vendor, procedure..etc) greatly appreciated. Thanks

[Histonet] BRAF

Hi Hisonetters Looking for information on BRAF-(V600E) IHC. Any information (vendor, procedure..etc) greatly appreciated. Thanks. ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histone

[Histonet] BRAF IHC

Is anyone doing BRAF IHC staining that is willing to share protocol details? TIA, Pat ___ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet

[Histonet] BRAF Mutation test

Hello Histonetters, We are looking to start BRAF mutation tests for IHC on paraffin sections. I would be grateful if you could let me know any preferred antibody clones for this test. Thank you in advance! Young Kwun Senior Hospital Scientist Immunohistochemistry Dept. of Ana