Re: [Histonet] Bone Histology Protocol

2014-03-01 Thread Jack Ratliff
Trevor, May I ask first if there is a particular reason why you are wanting to decalcify? Have you ever considered resin/plastic embedding of non-decalcified bone? Also, what type/species of bone are you wanting to process? My name is Jack Ratliff and I Chair the Hard Tissue Committee for the

RE: [Histonet] Bone Histology Protocol

2014-03-01 Thread Wait, Trevor Jordan
Subject: Re: [Histonet] Bone Histology Protocol Trevor, May I ask first if there is a particular reason why you are wanting to decalcify? Have you ever considered resin/plastic embedding of non-decalcified bone? Also, what type/species of bone are you wanting to process? My name is Jack Ratliff

RE: [Histonet] Bone Histology Protocol

2014-03-01 Thread Wait, Trevor Jordan
To: Wait, Trevor Jordan Cc: histonet@lists.utsouthwestern.edu; Jack Ratliff Subject: Re: [Histonet] Bone Histology Protocol Trevor, May I ask first if there is a particular reason why you are wanting to decalcify? Have you ever considered resin/plastic embedding of non-decalcified bone? Also, what

[Histonet] Bone Histology Protocol

2014-02-28 Thread Wait, Trevor Jordan
Hello colleagues! I'm currently trying to construct a complete Bone Processing Protocol that includes fixation (10%NBF), decalcification (EDTA), Dehydration, Clearing of the decalcificant (Xylene), infiltration, and Embedding in Paraffin. I would like to look at some procedures just to get a