Hi everyone,
Someone in the lab wishes to look at cultured cells in paraffin embedded cell
blocks. These cells grow mostly in non-adherent clumps or cell bodies, which
need to be preserved. Sections will be stained by IHC.
I've been looking into the matter, and it seems there is not really a
, September 05, 2013 12:45 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cell Block Preparation
I am getting complaints in regard to insufficient cell blocks. We currently
spin, pour off the supernatant, retrieve the sediment and process in lens paper.
Does anyone have a more current
[mailto:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Ann Specian
Sent: Thursday, September 05, 2013 12:45 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cell Block Preparation
I am getting complaints in regard to insufficient cell blocks. We currently
spin, pour off
'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Cell Block Preparation
This is how we do it now. In the old days, we used agar and to my mind, it is
still the best way when you have scant material.
- Spin in a conical tube and pour off
- Melt an agar slant (we get TSA slant from micro
Of
Rathborne, Toni
Sent: Friday, September 06, 2013 7:18 AM
To: 'Tom McNemar'; 'Ann Specian'; histonet@lists.utsouthwestern.edu
Subject: RE: [Histonet] Cell Block Preparation
I wonder if this method could be used with the product Histogel. Has
anyone tried it?
-Original Message-
From: histonet-boun
This is a pretty good method for scant specimens. I have even used it for CSFs
that have malignancy with success.
http://www.jove.com/video/1316/cell-block-preparation-from-cytology-specimen-with-predominance
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This e-mail message, including all attachments, is for the
12:45 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cell Block Preparation
I am getting complaints in regard to insufficient cell blocks. We
currently spin, pour off the supernatant, retrieve the sediment and
process in lens paper.
Does anyone have a more current technique which
, September 05, 2013 12:45 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cell Block Preparation
I am getting complaints in regard to insufficient cell blocks. We
currently spin, pour off the supernatant, retrieve the sediment and
process in lens paper.
Does anyone have a more
[mailto:thisis...@aol.com]
Sent: Thursday, September 05, 2013 12:45 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Cell Block Preparation
I am getting complaints in regard to insufficient cell blocks. We
currently spin, pour off the supernatant, retrieve the sediment and
process in lens
I am getting complaints in regard to insufficient cell blocks. We currently
spin, pour off the supernatant, retrieve the sediment and process in lens paper.
Does anyone have a more current technique which renders better cellularity?
Also, do you know which renders a better cell block: a
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