:histonet-boun...@lists.utsouthwestern.edu] On Behalf Of Patten,
Nicole (NIH/NIAAA) [F]
Sent: Thursday, June 04, 2009 9:55 AM
To: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] Cresyl Violet Counterstain of LFB
Hi Histonet... Quick question!!
I'm doing Luxol Fast Blue s
-0400
From: "Patten, Nicole (NIH/NIAAA) [F]"
Subject: [Histonet] Cresyl Violet Counterstain of LFB
To: "'histonet@lists.utsouthwestern.edu'"
Message-ID:
Content-Type: text/plain; charset="us-ascii"
Hi Histonet... Quick question!!
I'm d
oning and usually they are due
to the time it takes to freeze the tissue and the freezing temp. also.
René J.
--- On Thu, 6/4/09, Patten, Nicole (NIH/NIAAA) [F]
wrote:
From: Patten, Nicole (NIH/NIAAA) [F]
Subject: [Histonet] Cresyl Violet Counterstain of LFB
To: "'histonet@lists.uts
Hi Histonet... Quick question!!
I'm doing Luxol Fast Blue staining on frozen human brain sections (1% LFB in
95% EtOH with Hydroquinone/Sodium Sulfite Differentiator) and trying to
counterstain with Cresyl Violet. Following Cresyl Violet incubation (0.1% for
5') my protocol calls for me to dehy
